Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.2491
Description:hydroxylacyl-Coenzyme A dehydrogenase
Best-Of-UniGene (BOU) SequenceNM_008212
Genomic Coordiantes Displayed:Bases 8779800 to 8837200 of contig GA_x5J8B7W6U34
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.2491.jpeg
Best sense EST/protein match:AA254925 matched gb|AAK54642.1| (AF375597) medium and short chain L-3-hydroxyacyl-Coenzyme A (E = e-101)
Best antisense EST/protein match:AA674342 matched ref|XP_072328.1| (XM_072328) hypothetical protein XP_072328 [Homo sapiens] (E = 2e-18)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA674342 cDNA clone IMAGE:1092834 muscle 5' read Protein similarity
  AA538506 cDNA clone IMAGE:920436 pool 5' read Protein similarity
  BI158800 cDNA clone IMAGE:5062030 tumor, gross tissue 5' read
  BF539116 cDNA clone IMAGE:4190563 salivary gland 5' read
  AA544882 cDNA clone IMAGE:948879 mammary gland 5' read
  AA537201 cDNA clone IMAGE:949575 mammary gland 5' read
  BI986739 cDNA clone (no-name) neural retina
  BG869316 cDNA clone IMAGE:4920367 salivary gland 5' read
  BI152101 cDNA clone IMAGE:5067085 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI102107 cDNA clone IMAGE:5043301 kidney 5' read
  AA222494 cDNA clone IMAGE:661038 embryo 5' read
  W61719 cDNA clone IMAGE:372535 embryo 5' read Polyadenylation signal
  AI182631 cDNA clone IMAGE:1399298 mammary gland 5' read
  AA240796 cDNA clone IMAGE:670945 embryo 5' read
  BM052513 cDNA clone (no-name) 5' read
  AA763538 cDNA clone IMAGE:1067359 mammary gland 5' read
  BE912621 cDNA clone IMAGE:3963807 tumor, biopsy sample 5' read
  AA457984 cDNA clone IMAGE:849476 mammary gland 5' read
  BE912024 cDNA clone IMAGE:3962975 tumor, biopsy sample 5' read Polyadenylation signal

SENSE ESTs

  AU051540 cDNA clone MNCb-2159 brain 5' read Protein similarity
  BB574372 cDNA clone 5033403I06 ovary and uterus Protein similarity
  AU051214 cDNA clone MNCb-1681 brain 5' read Protein similarity
  BG803449 cDNA clone (no-name) neural retina
  BG806230 cDNA clone (no-name) neural retina
  BI330440 cDNA clone IMAGE:5136060 liver 5' read Protein similarity
  AA272846 cDNA clone IMAGE:747429 liver 5' read Protein similarity
  AA254925 cDNA clone IMAGE:720083 liver 5' read Protein similarity
  BG922147 cDNA clone IMAGE:4951077 infiltrating ductal carcinoma 5' read Protein similarity
  AW012074 cDNA clone IMAGE:2182295 kidney 5' read Protein similarity
  AI956702 cDNA clone IMAGE:2136405 kidney 5' read Protein similarity
  AI876084 cDNA clone IMAGE:1923837 liver 5' read Protein similarity
  BI683638 cDNA clone IMAGE:5342320 infiltrating ductal carcinoma 5' read Protein similarity
  BG142636 cDNA clone (no-name) total pancreas 5' read Protein similarity
  BG923995 cDNA clone IMAGE:4951100 infiltrating ductal carcinoma 5' read Protein similarity
  AA982959 cDNA clone IMAGE:1347610 mammary gland 5' read Protein similarity
  BG086728 cDNA clone H3129H03 5' read Protein similarity
  BF384514 cDNA clone IMAGE:4196106 liver 5' read Protein similarity
  BI082447 cDNA clone IMAGE:5009513 tumor, biopsy sample 5' read Protein similarity
  BG870734 cDNA clone IMAGE:4923465 salivary gland 5' read Protein similarity
  AA208922 cDNA clone IMAGE:676661 liver 5' read Protein similarity
  AA616856 cDNA clone IMAGE:904303 colon 5' read Protein similarity
  AA277681 cDNA clone IMAGE:737498 liver 5' read Protein similarity
  AA498151 cDNA clone IMAGE:889570 pool 5' read Protein similarity
  W67044 cDNA clone IMAGE:389014 embryo 5' read Protein similarity
  AA461903 cDNA clone IMAGE:851572 mammary gland 5' read Protein similarity
  BI694109 cDNA clone IMAGE:5370148 tumor, biopsy sample 5' read Protein similarity
  BG802451 cDNA clone (no-name) neural retina
  BI964663 cDNA clone (no-name) 5' read Protein similarity
  BF660165 cDNA clone IMAGE:3812399 liver 5' read Protein similarity
  BM053608 cDNA clone (no-name) 5' read
  BI440931 cDNA clone (no-name) 5' read Protein similarity
  BM022523 cDNA clone (no-name) 5' read Protein similarity
  AA832994 cDNA clone IMAGE:1433273 uterus 5' read Protein similarity
  W34588 cDNA clone IMAGE:352735 embryo 5' read Protein similarity
  W62688 cDNA clone IMAGE:372661 embryo 5' read Protein similarity
  AA563224 cDNA clone IMAGE:977129 embryo 5' read Protein similarity
  AA003140 cDNA clone IMAGE:426189 embryo 5' read Protein similarity
  AA110249 cDNA clone IMAGE:572283 kidney 5' read Protein similarity
  BI108542 cDNA clone IMAGE:5025260 tumor, gross tissue 5' read Protein similarity
  AA793973 cDNA clone IMAGE:1123061 muscle 5' read Protein similarity
  BF531611 cDNA clone IMAGE:4205683 colon 5' read Protein similarity
  BF140621 cDNA clone IMAGE:4014975 tumor, metastatic to mammary 5' read Protein similarity
  AA929169 cDNA clone IMAGE:1106561 colon 5' read Protein similarity
  W41951 cDNA clone IMAGE:353322 embryo 5' read Protein similarity
  AA691208 cDNA clone IMAGE:1164992 colon 5' read Protein similarity
  AA572011 cDNA clone IMAGE:875831 mammary gland 5' read
  AA546560 cDNA clone IMAGE:945296 embryo 5' read
  W47979 cDNA clone IMAGE:355538 embryo 5' read
  W53871 cDNA clone IMAGE:368571 embryo 5' read
  AI037326 cDNA clone IMAGE:1381114 mammary gland 5' read
  AA474740 cDNA clone IMAGE:804383 embryo 5' read
  AA546687 cDNA clone IMAGE:945369 embryo 5' read
  AA711857 cDNA clone IMAGE:1195694 mammary gland 5' read
  W97395 cDNA clone IMAGE:421936 embryo 5' read
  AW552111 cDNA clone L0208D11 3' read Polyadenylation signal
  BM053309 cDNA clone (no-name) 3' read Polyadenylation signal
  AA472354 cDNA clone IMAGE:874581 mammary gland 5' read
  AA472332 cDNA clone IMAGE:874557 mammary gland 5' read
  AW108401 cDNA clone IMAGE:2236013 kidney 3' read
  AI987752 cDNA clone IMAGE:2182295 kidney 3' read
  C77493 cDNA clone J0032E04 embryo 3' read Polyadenylation signal
  BG074015 cDNA clone H3129H03 3' read Polyadenylation signal
  AA980274 cDNA clone IMAGE:1348442 mammary gland 5' read
  BI440528 cDNA clone (no-name) 3' read Polyadenylation signal
  C79243 cDNA clone J0063B01 embryo 3' read
  BI964444 cDNA clone (no-name) 3' read Polyadenylation signal
  AA177876 cDNA clone IMAGE:619837 spleen 5' read
  AI048025 cDNA clone IMAGE:1021850 embryo 5' read
  BG142035 cDNA clone (no-name) total pancreas 3' read Polyadenylation signal
  AA451465 cDNA clone IMAGE:850237 mammary gland 5' read
  AI786416 cDNA clone IMAGE:1923837 liver 3' read Polyadenylation signal
  AA638521 cDNA clone IMAGE:1053704 colon 5' read
  AI790189 cDNA clone IMAGE:1973066 kidney 3' read
  W62926 cDNA clone IMAGE:385419 embryo 5' read
  AA033389 cDNA clone IMAGE:466204 embryo 5' read Polyadenylation signal
  BM022226 cDNA clone (no-name) 3' read Polyadenylation signal
  AA270603 cDNA clone IMAGE:736304 embryo 5' read
  BF136828 cDNA clone IMAGE:4011579 tumor, metastatic to mammary 5' read
  BI653081 cDNA clone IMAGE:5341222 tumor, gross tissue 5' read Polyadenylation signal
  AA122758 cDNA clone IMAGE:596183 spleen 5' read
  AI324327 cDNA clone IMAGE:596183 spleen 5' read
  AA016582 cDNA clone IMAGE:440190 embryo 5' read
  AI528627 cDNA clone IMAGE:596183 spleen 3' read Polyadenylation signal
  BB513794 cDNA clone D730047P18 mammary gland
  BB514432 cDNA clone D730050P18 mammary gland
  BB505775 cDNA clone D730004I23 mammary gland
  AA408505 cDNA clone C0029B03 embryo 3' read
  AV288010 cDNA clone 5033402O03 ovary and uterus