Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.25231
Description:Mus musculus mRNA for 53BP1 protein
Best-Of-UniGene (BOU) SequenceAJ414734
Genomic Coordiantes Displayed:Bases 4747009 to 4850870 of contig GA_x5J8B7W62CR
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.25231.jpeg
Best sense EST/protein match:AJ414734 matched emb|CAC94013.1| (AJ414734) 53BP1 protein [Mus musculus] (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA289551 cDNA clone IMAGE:749161 spleen 5' read Protein similarity
  AA200268 cDNA clone IMAGE:638663 spleen 5' read Protein similarity
  AA190165 cDNA clone IMAGE:637684 spleen 5' read Protein similarity
  AA105135 cDNA clone IMAGE:571916 kidney 5' read Protein similarity
  AA103159 cDNA clone IMAGE:554101 embryo 5' read Protein similarity
  BE447272 cDNA clone IMAGE:3330315 spleen 3' read Protein similarityPolyadenylation signal
  AA240868 cDNA clone IMAGE:671573 embryo 5' read Polyadenylation signal
  BF662675 cDNA clone IMAGE:3823223 spleen 3' read Protein similarity

SENSE ESTs

  BF537557 cDNA clone IMAGE:4192491 salivary gland 5' read Protein similarityPolyadenylation signal
  BG064877 cDNA clone H3024H02 3' read Protein similarityPolyadenylation signal
  C86611 cDNA clone J0229H09 embryo 3' read Protein similarityPolyadenylation signal
  BI965556 cDNA clone (no-name) 3' read Protein similarityPolyadenylation signal
  AI448801 cDNA clone IMAGE:614064 embryo 3' read Protein similarityPolyadenylation signal
  AI507644 cDNA clone IMAGE:920246 pool 3' read Protein similarityPolyadenylation signal
  AU016002 cDNA clone J0719D12 embryo 3' read Protein similarityPolyadenylation signal
  W58843 cDNA clone IMAGE:371728 embryo 5' read Protein similarity
  BI964424 cDNA clone (no-name) 3' read Protein similarityPolyadenylation signal
  BI964392 cDNA clone (no-name) 3' read Protein similarityPolyadenylation signal
  BI105249 cDNA clone IMAGE:5038377 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  AA637256 cDNA clone IMAGE:1179612 mammary gland 5' read Protein similarity
  BE197584 cDNA clone IMAGE:3256986 tumor, gross tissue 3' read Protein similarityPolyadenylation signal
  AA968010 cDNA clone IMAGE:1617171 hypothalamus 5' read Protein similarity
  AW536294 cDNA clone G0102G04 3' read Protein similarityPolyadenylation signal
  BG062636 cDNA clone L0955F05 5' read Protein similarity
  BI965873 cDNA clone (no-name) 5' read Protein similarity
  BI964639 cDNA clone (no-name) 5' read Protein similarity
  BG800330 cDNA clone (no-name) neural retina
  BI964596 cDNA clone (no-name) 5' read Protein similarity
  BI738155 cDNA clone IMAGE:5369464 retina 5' read Protein similarity
  BG802420 cDNA clone (no-name) neural retina
  AA538224 cDNA clone IMAGE:920246 pool 5' read Protein similarity
  AI615366 cDNA clone IMAGE:920246 pool 5' read Protein similarity
  AI152748 cDNA clone IMAGE:1478488 uterus 5' read Protein similarity
  BI655428 cDNA clone IMAGE:5329171 tumor, gross tissue 5' read Protein similarity
  BI989772 cDNA clone (no-name) neural retina
  BG078205 cDNA clone H3024H02 5' read Protein similarity
  BI990001 cDNA clone (no-name) neural retina
  BE226967 cDNA clone IMAGE:3256986 tumor, gross tissue 5' read Protein similarity
  BI658797 cDNA clone IMAGE:5347546 tumor, gross tissue 5' read Protein similarity
  BF100198 cDNA clone IMAGE:3979746 tumor, biopsy sample 5' read Protein similarity
  AW761791 cDNA clone IMAGE:2803486 thymus 5' read Protein similarity
  AA155080 cDNA clone IMAGE:614064 embryo 5' read Protein similarity