Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.25634
Description:RIKEN cDNA 2410018M14 gene
Best-Of-UniGene (BOU) SequenceAK010552
Genomic Coordiantes Displayed:Bases 621600 to 673400 of contig GA_x5J8B7W531T
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.25634.jpeg
Best sense EST/protein match:AK010552 matched dbj|BAB27021.1| (AK010552) data source:SPTR, source key:Q9VVL6, (E = 8e-85)
Best antisense EST/protein match:BG808301 matched gb|AAH20051.1|AAH20051 (BC020051) Unknown (protein for MGC:28179) [Mus musculus] (E = e-117)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI747801 cDNA clone IMAGE:2064889 kidney 5' read
  AA934143 cDNA clone IMAGE:1166339 colon 5' read Polyadenylation signal
  BF579140 cDNA clone IMAGE:4215955 colon 5' read Protein similarity
  BI154207 cDNA clone IMAGE:5032827 tumor, gross tissue 5' read
  BG100877 cDNA clone IMAGE:3658053 spermatocytes, pooled from multiple mice 5' read Polyadenylation signal
  AW544970 cDNA clone C0188B04 3' read Polyadenylation signal
  BG064256 cDNA clone H3017E01 3' read Polyadenylation signal
  AW544040 cDNA clone C0177B05 3' read Polyadenylation signal
  BF227902 cDNA clone IMAGE:3671189 tumor, gross tissue 3' read Polyadenylation signal
  AW547699 cDNA clone L0025F06 3' read Polyadenylation signal
  AW209246 cDNA clone IMAGE:2598869 infiltrating ductal carcinoma 3' read Polyadenylation signal
  AW547779 cDNA clone L0026F06 3' read Polyadenylation signal
  AU017648 cDNA clone J0742A04 embryo 3' read Polyadenylation signal
  BF320807 cDNA clone IMAGE:3673063 infiltrating ductal carcinoma 3' read Polyadenylation signal
  BF319629 cDNA clone IMAGE:3664084 round spermatids, pooled from multiple mice 3' read Polyadenylation signal
  BI655709 cDNA clone IMAGE:5325577 tumor, gross tissue 5' read
  AI415717 cDNA clone IMAGE:354327 embryo 3' read Polyadenylation signal
  BI660503 cDNA clone IMAGE:5349043 tumor, gross tissue 5' read
  AI551792 cDNA clone IMAGE:1065859 muscle 3' read
  AW413961 cDNA clone IMAGE:2650466 tumor, gross tissue 3' read
  AW741356 cDNA clone IMAGE:3154053 tumor, gross tissue 3' read
  AV286028 cDNA clone 5031417J19 ovary and uterus
  BI988630 cDNA clone (no-name) neural retina
  W98143 cDNA clone IMAGE:424156 embryo 5' read
  BF584811 cDNA clone IMAGE:4218496 colon 5' read
  AI427249 cDNA clone IMAGE:354327 embryo 5' read
  AA274605 cDNA clone IMAGE:746848 embryo 5' read
  AW414327 cDNA clone IMAGE:2650466 tumor, gross tissue 5' read
  AA270501 cDNA clone IMAGE:736639 embryo 5' read
  BF533270 cDNA clone IMAGE:4210470 liver 5' read
  BE377266 cDNA clone IMAGE:3593205 tumor, biopsy sample 5' read
  BG077608 cDNA clone H3017E01 5' read
  BG916200 cDNA clone IMAGE:4937283 tumor, gross tissue 5' read Protein similarity
  BI650204 cDNA clone IMAGE:5337168 tumor, gross tissue 5' read Protein similarity
  BF101576 cDNA clone IMAGE:3980879 tumor, biopsy sample 5' read Protein similarity
  BI733717 cDNA clone IMAGE:5359998 retina 5' read Protein similarity
  BF301315 cDNA clone IMAGE:4164951 salivary gland 5' read Protein similarity
  BI991056 cDNA clone (no-name) neural retina
  AI877248 cDNA clone IMAGE:1434552 uterus 5' read
  BF233289 cDNA clone IMAGE:4159092 liver 5' read Protein similarity
  BB609647 cDNA clone 1110032A17 5' read Protein similarity
  BF117423 cDNA clone IMAGE:3671189 tumor, gross tissue 5' read Protein similarity
  AI428989 cDNA clone IMAGE:1065859 muscle 5' read
  BE375678 cDNA clone IMAGE:3592458 tumor, biopsy sample 5' read Protein similarity
  AA619545 cDNA clone IMAGE:1065859 muscle 5' read
  BF147176 cDNA clone IMAGE:3657390 spermatocytes, pooled from multiple mice 5' read Protein similarity
  AW762120 cDNA clone IMAGE:3154053 tumor, gross tissue 5' read
  BI689538 cDNA clone IMAGE:5355194 infiltrating ductal carcinoma 5' read
  W42327 cDNA clone IMAGE:354327 embryo 5' read
  AW211561 cDNA clone IMAGE:2649049 tumor, gross tissue 5' read
  BG808301 cDNA clone (no-name) neural retina
  BF320789 cDNA clone IMAGE:3673062 infiltrating ductal carcinoma 5' read Protein similarity
  AW209466 cDNA clone IMAGE:2598869 infiltrating ductal carcinoma 5' read Protein similarity
  BF160533 cDNA clone IMAGE:3987696 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read

SENSE ESTs

  BI697176 cDNA clone IMAGE:5374337 tumor, biopsy sample 5' read
  W53478 cDNA clone IMAGE:371343 embryo 5' read
  AA050332 cDNA clone IMAGE:476837 embryo 5' read
  W58827 cDNA clone IMAGE:371624 embryo 5' read
  BF385233 cDNA clone IMAGE:4196940 liver 5' read
  BG975132 cDNA clone IMAGE:4979067 tumor, gross tissue 5' read
  AI747798 cDNA clone IMAGE:2064904 kidney 5' read Protein similarity
  AW044776 cDNA clone IMAGE:2182748 kidney 5' read
  BI082722 cDNA clone IMAGE:5009533 tumor, biopsy sample 5' read
  AA537604 cDNA clone IMAGE:945149 embryo 5' read
  AA035885 cDNA clone IMAGE:468771 embryo 5' read
  BI330117 cDNA clone IMAGE:5136341 liver 5' read
  BI686693 cDNA clone IMAGE:5352943 infiltrating ductal carcinoma 5' read
  AW240340 cDNA clone IMAGE:2655920 tumor, biopsy sample 5' read
  AA470173 cDNA clone IMAGE:849003 pool 5' read
  BM053724 cDNA clone (no-name) 5' read
  AA003139 cDNA clone IMAGE:426187 embryo 5' read
  AA036405 cDNA clone IMAGE:467856 embryo 5' read
  BM053408 cDNA clone (no-name) 3' read
  AI118654 cDNA clone IMAGE:1397765 mammary gland 5' read
  BM022573 cDNA clone (no-name) 3' read
  BI665404 cDNA clone IMAGE:5321938 infiltrating ductal carcinoma 5' read
  AW259608 cDNA clone IMAGE:2655920 tumor, biopsy sample 3' read
  AI790550 cDNA clone IMAGE:2064889 kidney 3' read Polyadenylation signal
  AW764059 cDNA clone IMAGE:3155658 tumor, gross tissue 3' read Polyadenylation signal