Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.27182
Description:RIKEN cDNA 1110031I02 gene
Best-Of-UniGene (BOU) SequenceBC017643
Genomic Coordiantes Displayed:Bases 5998000 to 6082000 of contig GA_x5J8B7W64TB
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.27182.jpeg
Best sense EST/protein match:BG922215 matched gb|AAH17643.1|AAH17643 (BC017643) Similar to hypothetical protein MGC4368 [Mus musculus] (E = 2e-93)
Best antisense EST/protein match:BI079969 matched gb|AAH18205.1|AAH18205 (BC018205) Unknown (protein for IMAGE:3464710) [Homo sapiens] (E = e-102)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BM070199 cDNA clone (no-name) 5' read Polyadenylation signal
  BI988730 cDNA clone (no-name) neural retina Polyadenylation signal
  W91324 cDNA clone IMAGE:423825 embryo 5' read
  AI614863 cDNA clone IMAGE:851655 mammary gland 5' read
  BI554925 cDNA clone IMAGE:5289461 tumor, gross tissue 5' read Protein similarity
  BG920681 cDNA clone IMAGE:4954670 infiltrating ductal carcinoma 5' read Protein similarity
  BG297582 cDNA clone IMAGE:4507431 retina 5' read
  BG519336 cDNA clone IMAGE:3497855 infiltrating ductal carcinoma 5' read Protein similarity
  BE290741 cDNA clone IMAGE:3497855 infiltrating ductal carcinoma 5' read
  AA500754 cDNA clone IMAGE:851655 mammary gland 5' read Protein similarity
  BE381365 cDNA clone IMAGE:3600349 tumor, biopsy sample 5' read Protein similarity
  BI079969 cDNA clone IMAGE:5007884 tumor, biopsy sample 5' read Protein similarity
  BF164373 cDNA clone IMAGE:3992255 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BG086946 cDNA clone H3132F02 5' read Protein similarity
  BI991101 cDNA clone (no-name) neural retina
  BI739676 cDNA clone IMAGE:5369074 retina 5' read Protein similarity
  AA124250 cDNA clone IMAGE:579399 kidney 5' read
  AW552679 cDNA clone L0216B03 3' read Polyadenylation signal
  AI510534 cDNA clone IMAGE:579399 kidney 5' read
  AI608318 cDNA clone IMAGE:849751 mammary gland 5' read
  BF582632 cDNA clone IMAGE:4208405 colon 5' read
  AA458199 cDNA clone IMAGE:849751 mammary gland 5' read
  BG074241 cDNA clone H3132F02 3' read Polyadenylation signal
  AW553835 cDNA clone L0232D05 3' read Polyadenylation signal
  BI157388 cDNA clone IMAGE:5063972 tumor, gross tissue 5' read
  AW475785 cDNA clone IMAGE:2922942 forebrain 3' read Polyadenylation signal
  AI452322 cDNA clone IMAGE:579399 kidney 3' read Polyadenylation signal
  AI481315 cDNA clone IMAGE:849751 mammary gland 3' read Polyadenylation signal
  AV285141 cDNA clone 5031408D23 ovary and uterus
  AI481765 cDNA clone IMAGE:851655 mammary gland 3' read Polyadenylation signal
  C85079 cDNA clone J0202A11 embryo 3' read

SENSE ESTs

  BI648835 cDNA clone IMAGE:5317629 tumor, gross tissue 5' read
  BG078906 cDNA clone H3033F01 5' read Protein similarity
  BG966964 cDNA clone IMAGE:4988803 colon 5' read
  BE634713 cDNA clone IMAGE:3413085 spleen 3' read
  BE633334 cDNA clone IMAGE:3413085 spleen 5' read
  AI481785 cDNA clone IMAGE:851814 mammary gland 3' read Protein similarity
  AI593571 cDNA clone IMAGE:1195505 mammary gland 3' read
  AI614884 cDNA clone IMAGE:851814 mammary gland 5' read Protein similarity
  AA499635 cDNA clone IMAGE:851814 mammary gland 5' read Protein similarity
  AU043030 cDNA clone J1037F10 3' read
  BF608502 cDNA clone ICRFp522D0573 whole embryo 5' read
  AA241078 cDNA clone IMAGE:655912 kidney 5' read
  BG872010 cDNA clone IMAGE:4924061 salivary gland 5' read
  AA144264 cDNA clone IMAGE:597424 spleen 5' read
  BF168677 cDNA clone IMAGE:4017046 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI646340 cDNA clone IMAGE:5316958 tumor, gross tissue 5' read
  BG971505 cDNA clone IMAGE:4972349 kidney 5' read
  AA245552 cDNA clone IMAGE:699508 pool 5' read
  AA690764 cDNA clone IMAGE:1195505 mammary gland 5' read
  BB563711 cDNA clone 1210001J05 lung 5' read
  AI099041 cDNA clone IMAGE:1396910 mammary gland 5' read
  BI692050 cDNA clone IMAGE:5352025 infiltrating ductal carcinoma 5' read Polyadenylation signal
  BE633142 cDNA clone IMAGE:3412826 spleen 5' read
  BE852036 cDNA clone IMAGE:3416580 spleen 3' read Polyadenylation signal
  BE285201 cDNA clone IMAGE:3491335 tumor, gross tissue 5' read
  BG872322 cDNA clone IMAGE:4922375 salivary gland 5' read
  AW907449 cDNA clone IMAGE:2812476 tumor, gross tissue 3' read Polyadenylation signal
  BE199313 cDNA clone IMAGE:3256383 tumor, gross tissue 3' read Polyadenylation signal
  BE850166 cDNA clone IMAGE:3416580 spleen 5' read
  AW324478 cDNA clone IMAGE:2616440 infiltrating ductal carcinoma 3' read Polyadenylation signal
  AW212904 cDNA clone IMAGE:2647762 tumor, biopsy sample 3' read Polyadenylation signal
  BF136092 cDNA clone IMAGE:4011416 tumor, metastatic to mammary 5' read
  AW542339 cDNA clone C0156D08 3' read Polyadenylation signal
  BG064018 cDNA clone H3014E09 3' read Polyadenylation signal
  AI159418 cDNA clone IMAGE:1448005 mammary gland 5' read
  AI182650 cDNA clone IMAGE:1399322 mammary gland 5' read
  AW412143 cDNA clone IMAGE:2812476 tumor, gross tissue 5' read
  W91510 cDNA clone IMAGE:423948 embryo 5' read
  BF135611 cDNA clone IMAGE:4009122 tumor, metastatic to mammary 5' read
  BI455689 cDNA clone IMAGE:5253333 tumor, gross tissue 5' read
  AI466575 cDNA clone IMAGE:438351 embryo 5' read
  AA008154 cDNA clone IMAGE:438351 embryo 5' read
  BF140748 cDNA clone IMAGE:4015968 tumor, metastatic to mammary 5' read