Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.28223
Description:inhibitor of DNA binding 4
Best-Of-UniGene (BOU) SequenceNM_031166
Genomic Coordiantes Displayed:Bases 1642741 to 1649458 of contig GA_x5J8B7W8864
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.28223.jpeg
Best sense EST/protein match:NM_031166 matched ref|NP_112443.1| (NM_031166) inhibitor of DNA binding 4 [Mus musculus] (E = 8e-62)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA008963 cDNA clone IMAGE:441175 embryo 5' read Polyadenylation signal
  W48392 cDNA clone IMAGE:355618 embryo 5' read Polyadenylation signal
  W48116 cDNA clone IMAGE:355929 embryo 5' read
  W64286 cDNA clone IMAGE:386419 embryo 5' read
  AI894046 cDNA clone IMAGE:441175 embryo 5' read
  AA718865 cDNA clone IMAGE:1178241 mammary gland 5' read
  AA222769 cDNA clone IMAGE:660546 embryo 5' read
  W77144 cDNA clone IMAGE:400085 embryo 5' read
  AI323287 cDNA clone IMAGE:440922 embryo 3' read
  AI323288 cDNA clone IMAGE:441175 embryo 3' read
  AA108736 cDNA clone IMAGE:571232 kidney 5' read
  AI587755 cDNA clone IMAGE:571232 kidney 5' read

SENSE ESTs

  AI838339 cDNA clone UI-M-AO0-aby-h-06-0-UI 3' read Polyadenylation signal
  BE281730 cDNA clone IMAGE:3492156 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF168526 cDNA clone IMAGE:4016983 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AI415677 cDNA clone IMAGE:353674 embryo 3' read Polyadenylation signal
  BG060830 cDNA clone L0908F06 3' read Polyadenylation signal
  BG061606 cDNA clone L0908F06 5' read
  BE859691 cDNA clone UIM-AO0-aby-h-06-0-UI- 5' read
  BG062463 cDNA clone L0953A06 5' read
  BG061892 cDNA clone L0953A12 3' read
  AI430921 cDNA clone IMAGE:478282 embryo 3' read
  AA049676 cDNA clone IMAGE:478282 embryo 5' read
  AI510060 cDNA clone IMAGE:478282 embryo 5' read
  AI666821 cDNA clone IMAGE:694693 lung 3' read Protein similarity
  AA218438 cDNA clone IMAGE:694693 lung 5' read