Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.28410
Description:RIKEN cDNA 2810473M14 gene
Best-Of-UniGene (BOU) SequenceAK013404
Genomic Coordiantes Displayed:Bases 2826866 to 2831027 of contig GA_x5J8B7W7RRJ
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.28410.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW323236 cDNA clone IMAGE:2646690 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI159342 cDNA clone IMAGE:5059878 tumor, gross tissue 5' read
  AA797875 cDNA clone IMAGE:1245475 mammary gland 5' read
  AA035907 cDNA clone IMAGE:468757 embryo 5' read
  BI466544 cDNA clone (no-name) pancreas 5' read
  BG081819 cDNA clone H3069D05 5' read
  AA035908 cDNA clone IMAGE:468759 embryo 5' read
  AL364401 cDNA clone B9C20C39 embryo 5' read
  AA176012 cDNA clone IMAGE:620067 spleen 5' read
  BI466316 cDNA clone (no-name) pancreas 3' read Polyadenylation signal
  BI081559 cDNA clone IMAGE:5011549 tumor, biopsy sample 5' read
  BG068794 cDNA clone H3069D05 3' read Polyadenylation signal
  AU023822 cDNA clone J0439E04 unfertilized egg 3' read Polyadenylation signal

SENSE ESTs

  AW320079 cDNA clone IMAGE:2395330 kidney 3' read
  AA987171 cDNA clone IMAGE:1432023 kidney 3' read Polyadenylation signal
  AA560864 cDNA clone IMAGE:977466 embryo 5' read
  AA543967 cDNA clone IMAGE:934285 embryo 5' read
  AI120085 cDNA clone IMAGE:1399202 mammary gland 5' read
  AA272048 cDNA clone IMAGE:737029 embryo 5' read
  W36146 cDNA clone p169.18 brain
  W36148 cDNA clone p169.24 brain