Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.28586
Description:upregulated during skeletal muscle growth 3
Best-Of-UniGene (BOU) SequenceNM_031400
Genomic Coordiantes Displayed:Bases 534514 to 536362 of contig GA_x5J8B7W5JQ8
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.28586.jpeg
Best sense EST/protein match:NM_031400 matched ref|NP_113577.1| (NM_031400) upregulated during skeletal muscle growth 3 [Mus (E = 2e-25)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA574731 cDNA clone IMAGE:991801 embryo 5' read
  AA870269 cDNA clone IMAGE:1094175 5' read
  AA561107 cDNA clone IMAGE:977533 embryo 5' read
  AW211885 cDNA clone IMAGE:2649460 tumor, gross tissue 5' read
  AA058012 cDNA clone IMAGE:480248 embryo 5' read
  AA529433 cDNA clone IMAGE:905879 embryo 5' read
  AA451232 cDNA clone IMAGE:850388 mammary gland 5' read
  BE635238 cDNA clone IMAGE:3411474 spleen 5' read
  BF321989 cDNA clone IMAGE:3411474 spleen 3' read
  AV222265 cDNA clone 3732415G01 embryonic body below diaphragm region
  AA183612 cDNA clone IMAGE:622263 spleen 5' read
  BF162334 cDNA clone IMAGE:3990727 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI079094 cDNA clone IMAGE:5005171 tumor, biopsy sample 5' read
  BI149776 cDNA clone IMAGE:5012168 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AI153769 cDNA clone IMAGE:1449186 mammary gland 5' read
  AA003327 cDNA clone IMAGE:427361 embryo 5' read
  W34544 cDNA clone IMAGE:352653 embryo 5' read
  AA832974 cDNA clone IMAGE:1433225 uterus 5' read
  BF119222 cDNA clone IMAGE:3986766 tumor, gross tissue 5' read
  AW558861 cDNA clone L0301A05 3' read Polyadenylation signal
  AW741692 cDNA clone IMAGE:3154534 tumor, gross tissue 3' read Polyadenylation signal
  BB511188 cDNA clone D730032M20 mammary gland
  AA117256 cDNA clone IMAGE:539152 embryo 5' read
  AU015697 cDNA clone J0715C05 embryo 3' read Polyadenylation signal
  AW554644 cDNA clone L0243E07 3' read Polyadenylation signal
  BI790408 cDNA clone (no-name) 3' read Polyadenylation signal
  AW555215 cDNA clone L0251E01 3' read Polyadenylation signal
  AW555220 cDNA clone L0251E07 3' read Polyadenylation signal
  AW557794 cDNA clone L0286E06 3' read Polyadenylation signal
  BF322953 cDNA clone IMAGE:3813299 brain 3' read Polyadenylation signal
  BG294736 cDNA clone IMAGE:4504011 retina 5' read Polyadenylation signal
  AW212410 cDNA clone IMAGE:2649460 tumor, gross tissue 3' read Polyadenylation signal
  AA606792 cDNA clone IMAGE:1005533 embryo 5' read
  BF181436 cDNA clone IMAGE:4036780 tumor, gross tissue 5' read Polyadenylation signal
  BI694589 cDNA clone IMAGE:5375328 tumor, biopsy sample 5' read
  BI499145 cDNA clone (no-name) pancreas 5' read Polyadenylation signal

SENSE ESTs