Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.29014
Description:T-cell lymphoma invasion and metastasis 2
Best-Of-UniGene (BOU) SequenceNM_011878
Genomic Coordiantes Displayed:Bases 352215 to 578915 of contig GA_x5J8B7W5Y5H
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.29014.jpeg
Best sense EST/protein match:NM_011878 matched ref|NP_036008.1| (NM_011878) T-cell lymphoma invasion and metastasis 2; RAS-related C3 (E = 0.0)
Best antisense EST/protein match:BF607288 matched ref|XP_011456.1| (XM_011456) CGI-75 protein [Homo sapiens] (E = e-100)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI591589 cDNA clone IMAGE:1165492 colon 3' read Polyadenylation signal
  AW551319 cDNA clone L0076G12 3' read Protein similarityPolyadenylation signal
  BG073668 cDNA clone H3126F08 3' read Polyadenylation signal
  AA710054 cDNA clone IMAGE:1165492 colon 5' read
  BF134885 cDNA clone IMAGE:4007324 tumor, metastatic to mammary 5' read Protein similarity
  AA270309 cDNA clone IMAGE:735608 embryo 5' read Protein similarity
  BF607288 cDNA clone ICRFp522O0944 whole embryo 5' read Protein similarity
  AI197511 cDNA clone IMAGE:1449879 uterus 5' read Protein similarity
  BG086477 cDNA clone H3126F08 5' read Protein similarity
  BI651622 cDNA clone IMAGE:5338327 tumor, gross tissue 5' read Protein similarity
  BB611112 cDNA clone 2810005G14 5' read Protein similarity

SENSE ESTs

  BB849794 cDNA clone F930103D15 inner ear 5' read
  BG081645 cDNA clone H3067E02 5' read Protein similarity
  BG068616 cDNA clone H3067E02 3' read Protein similarityPolyadenylation signal
  AU023377 cDNA clone J0431A12 unfertilized egg 3' read Protein similarityPolyadenylation signal
  AU023379 cDNA clone J0431B09 unfertilized egg 3' read Polyadenylation signal
  AA537475 cDNA clone IMAGE:949774 mammary gland 5' read Protein similarity
  AI607602 cDNA clone IMAGE:949774 mammary gland 5' read Protein similarity
  AA154436 cDNA clone IMAGE:605120 embryonal carcinoma 5' read Protein similarity