Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.30083
Description:RIKEN cDNA 2510015F01 gene
Best-Of-UniGene (BOU) SequenceBC011230
Genomic Coordiantes Displayed:Bases 2908979 to 2925768 of contig GA_x5J8B7W38LP
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.30083.jpeg
Best sense EST/protein match:BC011230 matched ref|NP_075059.1| (NM_022908) hypothetical protein FLJ12442 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:BG803514 matched dbj|BAA13377.1| (D87433) KIAA0246 protein [Homo sapiens] (E = e-100)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BM054653 cDNA clone (no-name) 5' read Polyadenylation signal
  BG083993 cDNA clone H3095B05 5' read
  BI966479 cDNA clone (no-name) 3' read Polyadenylation signal
  BF225878 cDNA clone IMAGE:3663004 tumor, metastatic to mammary 3' read Polyadenylation signal
  BF227794 cDNA clone IMAGE:3670939 tumor, gross tissue 3' read Polyadenylation signal
  BG071140 cDNA clone H3095B05 3' read Polyadenylation signal
  AU040095 cDNA clone J0800A01 3' read Protein similarityPolyadenylation signal
  AA409101 cDNA clone C0037A04 embryo 3' read Protein similarityPolyadenylation signal
  BE374555 cDNA clone IMAGE:3583736 tumor, biopsy sample 5' read
  AI641915 cDNA clone IMAGE:1097600 bowel 5' read Protein similarity
  AI664336 cDNA clone IMAGE:1497852 uterus 5' read Protein similarity
  BI144649 cDNA clone IMAGE:5051312 liver 5' read Protein similarity
  AA396958 cDNA clone IMAGE:693056 liver 5' read Protein similarity
  AA111650 cDNA clone IMAGE:555616 embryo 5' read Protein similarity
  AA869250 cDNA clone IMAGE:1097600 bowel 5' read