Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.30189
Description:RIKEN cDNA 2610510N21 gene
Best-Of-UniGene (BOU) SequenceAK012102
Genomic Coordiantes Displayed:Bases 1288200 to 1293800 of contig GA_x5J8B7W62RU
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.30189.jpeg
Best sense EST/protein match:AK012102 matched ref|NP_478126.1| (NM_058219) homolog of yeast mRNA transport regulator 3 [Homo (E = 3e-99)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  W91403 cDNA clone IMAGE:421835 embryo 5' read
  W59332 cDNA clone IMAGE:373944 embryo 5' read
  AA967692 cDNA clone IMAGE:1616842 hypothalamus 5' read
  AI118824 cDNA clone IMAGE:1397952 mammary gland 5' read
  W62144 cDNA clone IMAGE:375322 embryo 5' read
  AA000312 cDNA clone IMAGE:425696 embryo 5' read

SENSE ESTs

  AI597028 cDNA clone IMAGE:891393 mammary gland 3' read Protein similarity
  AA041911 cDNA clone IMAGE:475434 embryo 5' read
  AI509942 cDNA clone IMAGE:475434 embryo 5' read Protein similarity
  AI427443 cDNA clone IMAGE:475434 embryo 3' read Protein similarity
  BG800231 cDNA clone (no-name) neural retina
  W89684 cDNA clone IMAGE:420497 embryo 5' read