Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.30534
Description:RIKEN cDNA 4930584N22 gene
Best-Of-UniGene (BOU) SequenceBC019149
Genomic Coordiantes Displayed:Bases 1984444 to 2106277 of contig GA_x5J8B7W3BSW
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.30534.jpeg
Best sense EST/protein match:BC019149 matched gb|AAH19149.1|AAH19149 (BC019149) Unknown (protein for MGC:29168) [Mus musculus] (E = 0.0)
Best antisense EST/protein match:BE291721 matched dbj|BAB31871.1| (AK019829) data source:SPTR, source key:Q9H846, (E = e-102)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF142819 cDNA clone IMAGE:4021435 tumor, metastatic to mammary 5' read Protein similarity
  BI155607 cDNA clone IMAGE:5033668 tumor, gross tissue 5' read Protein similarity
  BG923471 cDNA clone IMAGE:4935073 infiltrating ductal carcinoma 5' read Protein similarity
  BI662161 cDNA clone IMAGE:5350914 tumor, gross tissue 5' read Protein similarity
  AA510123 cDNA clone IMAGE:891076 mammary gland 5' read Protein similarity
  BF780573 cDNA clone IMAGE:4221531 kidney 5' read Protein similarity
  BI688942 cDNA clone IMAGE:5353443 infiltrating ductal carcinoma 5' read Protein similarity
  AW320493 cDNA clone IMAGE:2609615 infiltrating ductal carcinoma 5' read Protein similarity
  AA967576 cDNA clone IMAGE:1616639 hypothalamus 5' read Protein similarity
  AI390214 cDNA clone IMAGE:660182 embryo 5' read Protein similarity
  BG917923 cDNA clone IMAGE:4949939 infiltrating ductal carcinoma 5' read Protein similarity
  AA220600 cDNA clone IMAGE:660182 embryo 5' read Protein similarity
  BF451990 cDNA clone IMAGE:3675591 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF119208 cDNA clone IMAGE:3986762 tumor, gross tissue 5' read
  BE291721 cDNA clone IMAGE:3498751 infiltrating ductal carcinoma 5' read Protein similarity
  W65828 cDNA clone IMAGE:387302 embryo 5' read
  AI430629 cDNA clone IMAGE:387302 embryo 5' read
  AA209731 cDNA clone IMAGE:676237 liver 5' read Protein similarity
  AW413606 cDNA clone IMAGE:2631690 infiltrating ductal carcinoma 3' read
  AA537610 cDNA clone IMAGE:945176 embryo 5' read
  BI697218 cDNA clone IMAGE:5374588 tumor, biopsy sample 5' read
  BB511238 cDNA clone D730032P22 mammary gland
  AI464326 cDNA clone IMAGE:660182 embryo 3' read

SENSE ESTs

  AA221815 cDNA clone IMAGE:697188 pool 5' read
  BI649886 cDNA clone IMAGE:5337199 tumor, gross tissue 5' read Protein similarity
  BI731852 cDNA clone IMAGE:5359650 retina 5' read Protein similarity
  BI149130 cDNA clone IMAGE:5053345 liver 5' read Protein similarity
  BG918700 cDNA clone IMAGE:4947564 infiltrating ductal carcinoma 5' read Protein similarity