Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.31008
Description:ESTs, Weakly similar to T12482 hypothetical protein DKFZp564P0662.1 [H.sapiens]
Best-Of-UniGene (BOU) SequenceBG245109
Genomic Coordiantes Displayed:Bases 303652 to 314260 of contig GA_x5J8B7W3U46
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.31008.jpeg
Best sense EST/protein match:AA269826 matched ref|XP_058487.1| (XM_058487) similar to cytoplasmic protein (H. sapiens) [Homo (E = e-101)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BE369061 cDNA clone IMAGE:3590598 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI902098 cDNA clone (no-name) 5' read Protein similarity
  BI903308 cDNA clone (no-name) 5' read Protein similarity
  BI903085 cDNA clone (no-name) 3' read
  BI901803 cDNA clone (no-name) 3' read Protein similarity

SENSE ESTs

  BG294901 cDNA clone IMAGE:4504177 retina 5' read
  AL118243 cDNA clone 528_11M10 dissected endoderm 5' read Polyadenylation signal
  AV287481 cDNA clone 5031436K13 ovary and uterus
  AI505611 cDNA clone IMAGE:1122679 muscle 3' read
  BF731267 cDNA clone IMAGE:3976821 marginal zone b-cell tumor 5' read Polyadenylation signal
  BG294872 cDNA clone IMAGE:4504176 retina 5' read
  AA260394 cDNA clone IMAGE:747159 liver 5' read
  AA681438 cDNA clone IMAGE:1122679 muscle 5' read Protein similarity
  AA537426 cDNA clone IMAGE:949710 mammary gland 5' read Protein similarity
  BI738162 cDNA clone IMAGE:5369466 retina 5' read Protein similarity
  BI731311 cDNA clone IMAGE:5360887 retina 5' read Protein similarity
  AI463854 cDNA clone IMAGE:736086 embryo 5' read Protein similarity
  BI465467 cDNA clone (no-name) pancreas 5' read Protein similarity
  AA269826 cDNA clone IMAGE:736086 embryo 5' read Protein similarity
  BI220660 cDNA clone IMAGE:5102077 liver 5' read Protein similarity
  BG915911 cDNA clone IMAGE:4937727 tumor, gross tissue 5' read Protein similarity