Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.32126
Description:RIKEN cDNA 3010001K23 gene
Best-Of-UniGene (BOU) SequenceBC005542
Genomic Coordiantes Displayed:Bases 179847 to 207595 of contig GA_x5J8B7W5YG7
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.32126.jpeg
Best sense EST/protein match:BC005542 matched gb|AAH05542.1|AAH05542 (BC005542) RIKEN cDNA 3010001K23 gene [Mus musculus] (E = 0.0)
Best antisense EST/protein match:BG087544 matched ref|NP_001338.1| (NM_001347) diacylglycerol kinase, theta (110kD); diacylglycerol (E = 6e-59)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BG087544 cDNA clone H3141A06 5' read Protein similarity
  AA030105 cDNA clone IMAGE:464344 embryo 5' read Protein similarity
  BI791231 cDNA clone (no-name) 5' read Protein similarity
  BI789604 cDNA clone (no-name) 5' read Protein similarity
  BI156993 cDNA clone IMAGE:5063581 tumor, gross tissue 5' read Protein similarity
  BI789580 cDNA clone (no-name) 5' read Protein similarity
  AA798626 cDNA clone IMAGE:1245672 mammary gland 5' read Protein similarity
  BI559174 cDNA clone IMAGE:5294246 tumor, gross tissue 5' read
  AA172606 cDNA clone IMAGE:619505 spleen 5' read
  AV288472 cDNA clone 5033411E23 ovary and uterus
  BB507416 cDNA clone D730012N23 mammary gland
  AI604074 cDNA clone IMAGE:619505 spleen 5' read
  BG296846 cDNA clone IMAGE:4506489 retina 5' read
  AW554815 cDNA clone L0245G12 3' read
  AW554695 cDNA clone L0244B10 3' read
  BG074923 cDNA clone H3141A06 3' read
  AI449196 cDNA clone IMAGE:619505 spleen 3' read
  BI790293 cDNA clone (no-name) 3' read
  BE334510 cDNA clone IMAGE:3257469 tumor, gross tissue 5' read
  AI448991 cDNA clone IMAGE:615565 embryo 3' read Polyadenylation signal


  BG294157 cDNA clone IMAGE:4503102 retina 5' read
  AV287464 cDNA clone 5031436G08 ovary and uterus
  AV286230 cDNA clone 5031420H04 ovary and uterus
  AA244696 cDNA clone IMAGE:679549 liver 5' read
  AA270197 cDNA clone IMAGE:748670 liver 5' read
  AI785339 cDNA clone IMAGE:1922424 kidney 3' read
  AA796506 cDNA clone IMAGE:1078178 colon 5' read
  AI647950 cDNA clone IMAGE:1971266 kidney 3' read
  AA184272 cDNA clone IMAGE:633558 embryonal carcinoma 5' read
  BI248015 cDNA clone IMAGE:5124492 liver 5' read
  BI103475 cDNA clone IMAGE:5044203 kidney 5' read
  BF584812 cDNA clone IMAGE:4218162 colon 5' read
  BG967613 cDNA clone IMAGE:4987682 colon 5' read
  BI082473 cDNA clone IMAGE:5009378 tumor, biopsy sample 5' read
  BE368951 cDNA clone IMAGE:3590497 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AA792136 cDNA clone IMAGE:1024722 muscle 5' read
  AI526594 cDNA clone IMAGE:1922424 kidney 5' read
  AI465819 cDNA clone IMAGE:1247022 mammary gland 5' read
  AA050724 cDNA clone IMAGE:476448 embryo 5' read
  AA050746 cDNA clone IMAGE:476472 embryo 5' read
  AL365009 cDNA clone S9322B46 embryo 5' read
  AA839130 cDNA clone IMAGE:1247022 mammary gland 5' read