Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.3616
Description:polymerase, gamma
Best-Of-UniGene (BOU) SequenceNM_017462
Genomic Coordiantes Displayed:Bases 13189000 to 13231000 of contig GA_x5J8B7W89HK
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.3616.jpeg
Best sense EST/protein match:AW907751 matched sp|P54099|DPG1_MOUSE DNA polymerase gamma subunit 1 (Mitochondrial DNA polymerase (E = e-106)
Best antisense EST/protein match:BI789458 matched dbj|BAA91770.1| (AK001581) unnamed protein product [Homo sapiens] (E = 9e-72)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA049048 cDNA clone IMAGE:479398 embryo 5' read Protein similarity
  W49207 cDNA clone IMAGE:355937 embryo 5' read Protein similarity
  AA238671 cDNA clone IMAGE:697673 pool 5' read Protein similarity
  AI429670 cDNA clone IMAGE:420278 embryo 3' read Protein similarity
  BI790207 cDNA clone (no-name) 3' read Protein similarityPolyadenylation signal
  AI430422 cDNA clone IMAGE:420278 embryo 5' read
  BI789458 cDNA clone (no-name) 5' read
  W89816 cDNA clone IMAGE:420278 embryo 5' read

SENSE ESTs

  AI159398 cDNA clone IMAGE:1447983 mammary gland 5' read
  BB565537 cDNA clone 2310052K21 tongue 5' read
  AW107099 cDNA clone IMAGE:2192616 kidney 5' read Protein similarity
  AU067367 cDNA clone MNCb-3360 brain 5' read Protein similarity
  BI158008 cDNA clone IMAGE:5061097 tumor, gross tissue 5' read
  AA245611 cDNA clone IMAGE:699330 pool 5' read
  BF581433 cDNA clone IMAGE:4223961 colon 5' read Protein similarity
  AI594249 cDNA clone IMAGE:1004924 embryo 5' read
  AA607085 cDNA clone IMAGE:1004924 embryo 5' read
  AI503064 cDNA clone IMAGE:1004924 embryo 3' read
  BE914627 cDNA clone IMAGE:3965073 tumor, biopsy sample 5' read Protein similarity