Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.41938
Description:DNA segment, Chr 12, ERATO Doi 482, expressed
Best-Of-UniGene (BOU) SequenceBB128804
Genomic Coordiantes Displayed:Bases 21861 to 27227 of contig GA_x5J8B7W37T2
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.41938.jpeg
Best sense EST/protein match:AA210539 matched ref|NP_077298.1| (NM_024322) hypothetical protein MGC11266 [Homo sapiens] (E = 4e-14)
Best antisense EST/protein match:AA880100 matched sp|P21932|CYA3_RAT ADENYLATE CYCLASE TYPE III (ADENYLATE CYCLASE, OLFACTIVE TYPE) (ATP (E = 8e-79)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AA880100 cDNA clone IMAGE:1230885 mammary gland 5' read Protein similarity
  AA114591 cDNA clone IMAGE:536784 embryo 5' read Protein similarity
  BG085448 cDNA clone H3113D06 5' read Protein similarity
  AA033133 cDNA clone IMAGE:465502 embryo 5' read
  BG694969 cDNA clone IMAGE:4317551 pituitary gland 5' read
  AA119630 cDNA clone IMAGE:554761 embryo 5' read
  AW546390 cDNA clone L0007F10 3' read Polyadenylation signal
  AW546381 cDNA clone L0007E06 3' read Polyadenylation signal
  BG072654 cDNA clone H3113D06 3' read Polyadenylation signal
  AI644555 cDNA clone IMAGE:554761 embryo 3' read Polyadenylation signal


  AA176041 cDNA clone IMAGE:619753 spleen 5' read
  AA289693 cDNA clone IMAGE:749350 spleen 5' read
  BG067679 cDNA clone H3057A05 3' read Polyadenylation signal
  BI715089 cDNA clone (no-name) 3' read Polyadenylation signal
  BI793358 cDNA clone (no-name) 3' read Polyadenylation signal
  C87113 cDNA clone J0239G04 embryo 3' read Polyadenylation signal
  AI019698 cDNA clone IMAGE:1365096 mammary gland 5' read
  AA210539 cDNA clone IMAGE:560206 embryo 5' read