Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.4449
Description:endonuclease G
Best-Of-UniGene (BOU) SequenceNM_007931
Genomic Coordiantes Displayed:Bases 9323910 to 9333609 of contig GA_x5J8B7W4QPD
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.4449.jpeg
Best sense EST/protein match:AI266810 matched ref|NP_031957.1| (NM_007931) endonuclease G [Mus musculus] (E = e-133)
Best antisense EST/protein match:BI648582 matched pir||T14751 hypothetical protein DKFZp566D143.1 - human (fragment) (E = 2e-98)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI151333 cDNA clone IMAGE:5067891 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI695521 cDNA clone IMAGE:5372481 tumor, biopsy sample 5' read Protein similarity
  BI648582 cDNA clone IMAGE:5318011 tumor, gross tissue 5' read Protein similarity
  BI646686 cDNA clone IMAGE:5320105 tumor, gross tissue 5' read Protein similarity
  BI736384 cDNA clone IMAGE:5366448 retina 5' read Protein similarity
  BI440870 cDNA clone (no-name) 5' read Protein similarity
  BG915254 cDNA clone IMAGE:4936911 tumor, gross tissue 5' read Protein similarity
  AI316100 cDNA clone IMAGE:1974422 pituitary 5' read Protein similarity
  BI217338 cDNA clone IMAGE:5096791 liver 5' read Protein similarity
  BG972751 cDNA clone IMAGE:4972911 kidney 5' read Protein similarity
  BG077826 cDNA clone H3021D08 5' read Protein similarity
  BG175931 cDNA clone IMAGE:4460916 tumor, biopsy sample 5' read Protein similarity
  BE374203 cDNA clone IMAGE:3586044 tumor, biopsy sample 5' read Protein similarity
  BI078604 cDNA clone IMAGE:5004767 tumor, biopsy sample 5' read Protein similarity
  BI247352 cDNA clone IMAGE:5125736 liver 5' read Protein similarity
  BI103723 cDNA clone IMAGE:5042443 kidney 5' read Protein similarity
  AA792457 cDNA clone IMAGE:1153322 muscle 5' read Protein similarity
  BI987530 cDNA clone (no-name) neural retina
  AA032916 cDNA clone IMAGE:465744 embryo 5' read
  BF318778 cDNA clone IMAGE:3657159 spermatocytes, pooled from multiple mice 5' read Protein similarity
  AA555705 cDNA clone IMAGE:908652 embryo 5' read
  AA242692 cDNA clone IMAGE:671895 embryo 5' read Protein similarity
  AA544219 cDNA clone IMAGE:933714 embryo 5' read Protein similarity
  AA410141 cDNA clone C0015C02 embryo 5' read Protein similarity
  AA049147 cDNA clone IMAGE:479449 embryo 5' read Protein similarity
  AW542021 cDNA clone C0153G05 3' read Protein similarityPolyadenylation signal
  AU040962 cDNA clone J0821F11 3' read Protein similarityPolyadenylation signal
  AW545262 cDNA clone C0191E09 3' read Protein similarityPolyadenylation signal
  AU040975 cDNA clone J0821H07 3' read Protein similarityPolyadenylation signal
  AW240238 cDNA clone IMAGE:2655847 tumor, biopsy sample 5' read Polyadenylation signal
  AW259565 cDNA clone IMAGE:2655847 tumor, biopsy sample 3' read Polyadenylation signal
  BG064577 cDNA clone H3021D08 3' read Protein similarityPolyadenylation signal
  C77242 cDNA clone J0027H08 embryo 3' read Protein similarityPolyadenylation signal
  AW541225 cDNA clone C0142E08 3' read Protein similarityPolyadenylation signal
  BE569812 cDNA clone IMAGE:3709943 infiltrating ductal carcinoma 5' read

SENSE ESTs

  AA562131 cDNA clone IMAGE:962628 embryo 5' read Protein similarityPolyadenylation signal
  AL033338 cDNA clone (no-name) embryonic region
  AI266810 cDNA clone IMAGE:1891207 liver 3' read Protein similarityPolyadenylation signal
  BI246468 cDNA clone IMAGE:5124193 liver 5' read Protein similarity
  AI226510 cDNA clone IMAGE:1891207 liver 5' read Protein similarity
  BG807166 cDNA clone (no-name) neural retina
  BI328925 cDNA clone IMAGE:5133326 liver 5' read Protein similarity
  BI112231 cDNA clone IMAGE:5029633 tumor, gross tissue 5' read Protein similarity
  BI222152 cDNA clone IMAGE:5102260 liver 5' read Protein similarity