Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.4501
Description:prostaglandin E receptor 1 (subtype EP1), 42kD
Best-Of-UniGene (BOU) SequenceNM_013641
Genomic Coordiantes Displayed:Bases 1246315 to 1251166 of contig GA_x5J8B7W3E7T
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.4501.jpeg
Best sense EST/protein match:NM_013641 matched ref|NP_038669.1| (NM_013641) prostaglandin E receptor EP1 subtype [Mus musculus] (E = e-139)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG694486 cDNA clone IMAGE:4316456 pituitary gland 5' read
  AL361480 cDNA clone A9C29D62 embryo 5' read
  AA039098 cDNA clone IMAGE:474714 embryo 5' read
  BG966551 cDNA clone IMAGE:4987458 colon 5' read
  BG803448 cDNA clone (no-name) neural retina
  BI651118 cDNA clone IMAGE:5338244 tumor, gross tissue 5' read Protein similarity
  AA510635 cDNA clone IMAGE:863536 mammary gland 5' read
  BF123430 cDNA clone IMAGE:4023751 tumor, gross tissue 5' read Protein similarity
  BG261811 cDNA clone IMAGE:4481448 retina 5' read Protein similarity
  BF534158 cDNA clone IMAGE:4187074 salivary gland 5' read Protein similarity
  AA590749 cDNA clone IMAGE:990448 embryo 5' read Protein similarity

SENSE ESTs

  BB603028 cDNA clone D730005P21 mammary gland Protein similarity
  BM054533 cDNA clone (no-name) 5' read
  BI966390 cDNA clone (no-name) 3' read