Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.45552
Description:RIKEN cDNA 1700030E05 gene
Best-Of-UniGene (BOU) SequenceBC017608
Genomic Coordiantes Displayed:Bases 926059 to 935996 of contig GA_x5J8B7W3TPM
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.45552.jpeg
Best sense EST/protein match:BC017608 matched ref|NP_076279.1| (NM_023790) RIKEN cDNA 1700030E05 [Mus musculus] (E = 0.0)
Best antisense EST/protein match:BG805910 matched ref|NP_033132.1| (NM_009106) rhotekin [Mus musculus] (E = 1e-37)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BE334443 cDNA clone IMAGE:3257380 tumor, gross tissue 5' read
  BI650685 cDNA clone IMAGE:5337656 tumor, gross tissue 5' read
  BI789872 cDNA clone (no-name) 5' read
  BE225923 cDNA clone (no-name) pancreatic bud 3' read
  BG805910 cDNA clone (no-name) neural retina
  BE533888 cDNA clone IMAGE:3597498 infiltrating ductal carcinoma 5' read
  BI790070 cDNA clone (no-name) 3' read Polyadenylation signal
  AW910156 cDNA clone IMAGE:3157509 infiltrating ductal carcinoma 3' read Polyadenylation signal
  BE691005 cDNA clone IMAGE:3411008 spleen 3' read Polyadenylation signal
  AW321818 cDNA clone IMAGE:2609590 infiltrating ductal carcinoma 3' read Polyadenylation signal


  AA034678 cDNA clone IMAGE:466059 embryo 5' read
  BG299251 cDNA clone IMAGE:4507671 retina 5' read
  BB850847 cDNA clone F930109J05 inner ear 5' read
  AA184563 cDNA clone IMAGE:633327 embryonal carcinoma 5' read
  BB850857 cDNA clone F930109K05 inner ear 5' read
  AA169997 cDNA clone IMAGE:614822 embryo 5' read
  BB849285 cDNA clone F930008H23 inner ear 5' read