Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.608
Description:RIKEN cDNA 2700089E24 gene
Best-Of-UniGene (BOU) SequenceAK005205
Genomic Coordiantes Displayed:Bases 23899 to 31774 of contig GA_x5J8B7W3HWT
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.608.jpeg
Best sense EST/protein match:AK005205 matched dbj|BAB23883.1| (AK005205) evidence:NAS~hypothetical protein~putative [Mus (E = 1e-78)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA041690 cDNA clone IMAGE:474893 embryo 5' read Polyadenylation signal
  AA041704 cDNA clone IMAGE:474941 embryo 5' read
  AA048533 cDNA clone IMAGE:477570 embryo 5' read Polyadenylation signal
  W33425 cDNA clone IMAGE:352059 embryo 5' read
  AI131982 cDNA clone IMAGE:1400042 mammary gland 5' read
  AI593907 cDNA clone IMAGE:1121995 muscle 5' read
  AA637587 cDNA clone IMAGE:1121995 muscle 5' read
  AI608557 cDNA clone IMAGE:1121995 muscle 3' read

SENSE ESTs

  BE372907 cDNA clone IMAGE:3583265 tumor, biopsy sample 5' read
  AV286292 cDNA clone 5031421C18 ovary and uterus
  BE135371 cDNA clone IMAGE:1532817 uterus 5' read
  AA726791 cDNA clone IMAGE:1194106 muscle 5' read
  AA271517 cDNA clone IMAGE:737973 liver 5' read
  AI509063 cDNA clone IMAGE:737973 liver 5' read
  AA981555 cDNA clone IMAGE:1347401 mammary gland 5' read
  AA793048 cDNA clone IMAGE:1068880 mammary gland 5' read
  BG087540 cDNA clone H3141A02 5' read
  AA607344 cDNA clone IMAGE:1052246 colon 5' read
  BI454862 cDNA clone IMAGE:5251750 tumor, gross tissue 5' read
  AI159030 cDNA clone IMAGE:1333048 mammary gland 5' read
  BG920570 cDNA clone IMAGE:4953862 infiltrating ductal carcinoma 5' read
  BE304050 cDNA clone IMAGE:3499664 infiltrating ductal carcinoma 5' read
  BB617465 cDNA clone 5031431L04 ovary and uterus