Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.826
Description:phosphatidylinositol glycan, class F
Best-Of-UniGene (BOU) SequenceNM_008838
Genomic Coordiantes Displayed:Bases 6116200 to 6205800 of contig GA_x5J8B7W7FUK
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.826.jpeg
Best sense EST/protein match:BG081585 matched ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus] (E = e-100)
Best antisense EST/protein match:BI658912 matched ref|NP_036381.1| (NM_012249) ras-like protein [Homo sapiens] (E = e-106)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI557909 cDNA clone IMAGE:5290276 tumor, gross tissue 5' read Protein similarity
  BF179043 cDNA clone IMAGE:4037392 tumor, gross tissue 5' read
  BI159288 cDNA clone IMAGE:5062643 tumor, gross tissue 5' read
  BE135186 cDNA clone IMAGE:1532918 uterus 5' read Polyadenylation signal
  AA174394 cDNA clone IMAGE:616695 spleen 5' read Polyadenylation signal
  BI499800 cDNA clone (no-name) pancreas 3' read Polyadenylation signal
  BF180900 cDNA clone IMAGE:4037912 tumor, gross tissue 5' read Polyadenylation signal
  BI792520 cDNA clone (no-name) 5' read Polyadenylation signal
  BI081723 cDNA clone IMAGE:5011273 tumor, biopsy sample 5' read
  BE309564 cDNA clone IMAGE:3489521 tumor, gross tissue 5' read
  BI793375 cDNA clone (no-name) 3' read Polyadenylation signal
  BG918996 cDNA clone IMAGE:4948168 infiltrating ductal carcinoma 5' read
  BI500043 cDNA clone (no-name) pancreas 3' read Polyadenylation signal
  BF228174 cDNA clone IMAGE:3671568 tumor, gross tissue 3' read Polyadenylation signal
  BI439953 cDNA clone (no-name) 3' read Polyadenylation signal
  BI348705 cDNA clone (no-name) 3' read Polyadenylation signal
  BF451313 cDNA clone IMAGE:3674249 infiltrating ductal carcinoma 3' read Polyadenylation signal
  AI785002 cDNA clone IMAGE:1924605 liver 3' read Polyadenylation signal
  BF226355 cDNA clone IMAGE:3672457 infiltrating ductal carcinoma 3' read Polyadenylation signal
  BE309678 cDNA clone IMAGE:3490197 tumor, gross tissue 5' read
  BE686699 cDNA clone IMAGE:3415661 spleen 3' read Polyadenylation signal
  BE849156 cDNA clone IMAGE:3415661 spleen 5' read
  BF162696 cDNA clone IMAGE:3988597 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI440340 cDNA clone (no-name) 5' read
  AA636375 cDNA clone IMAGE:1120960 muscle 5' read
  BE307267 cDNA clone IMAGE:3496544 tumor, gross tissue 5' read
  BI657842 cDNA clone IMAGE:5329019 tumor, gross tissue 5' read
  BI735901 cDNA clone IMAGE:5367624 retina 5' read
  AA174617 cDNA clone IMAGE:620900 spleen 5' read
  BE570144 cDNA clone IMAGE:3709437 infiltrating ductal carcinoma 5' read
  BF320071 cDNA clone IMAGE:3672457 infiltrating ductal carcinoma 5' read
  BF321640 cDNA clone IMAGE:3673761 infiltrating ductal carcinoma 5' read
  BG173782 cDNA clone IMAGE:4457072 tumor, biopsy sample 5' read
  BI649264 cDNA clone IMAGE:5317797 tumor, gross tissue 5' read
  AA271510 cDNA clone IMAGE:762542 embryo 5' read
  BI158774 cDNA clone IMAGE:5061784 tumor, gross tissue 5' read
  BG922952 cDNA clone IMAGE:4952546 infiltrating ductal carcinoma 5' read
  BE199452 cDNA clone IMAGE:3256696 tumor, gross tissue 3' read
  AW212231 cDNA clone IMAGE:2649222 tumor, gross tissue 3' read
  BI693837 cDNA clone IMAGE:5370327 tumor, biopsy sample 5' read
  BI660223 cDNA clone IMAGE:5348495 tumor, gross tissue 5' read
  BI111716 cDNA clone IMAGE:5027956 tumor, gross tissue 5' read
  BF578406 cDNA clone IMAGE:4207199 colon 5' read
  BF167066 cDNA clone IMAGE:3994970 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF450642 cDNA clone IMAGE:3674249 infiltrating ductal carcinoma 5' read
  BF141174 cDNA clone IMAGE:4016562 tumor, metastatic to mammary 5' read
  AA672661 cDNA clone IMAGE:1067326 mammary gland 5' read
  BI150334 cDNA clone IMAGE:5065695 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BG872773 cDNA clone IMAGE:4924916 salivary gland 5' read
  BE368222 cDNA clone IMAGE:3590842 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI554817 cDNA clone IMAGE:5289862 tumor, gross tissue 5' read
  BG242829 cDNA clone IMAGE:4483793 tumor, biopsy sample 5' read
  BF141928 cDNA clone IMAGE:4021180 tumor, metastatic to mammary 5' read
  BI654372 cDNA clone IMAGE:5324457 tumor, gross tissue 5' read
  BI499358 cDNA clone (no-name) pancreas 5' read
  BI319937 cDNA clone (no-name) pancreas 5' read
  BI112174 cDNA clone IMAGE:5029857 tumor, gross tissue 5' read
  BF166177 cDNA clone IMAGE:4018638 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI319981 cDNA clone (no-name) pancreas 5' read
  BF117726 cDNA clone IMAGE:3671568 tumor, gross tissue 5' read
  BG921214 cDNA clone IMAGE:4954375 infiltrating ductal carcinoma 5' read
  BI158216 cDNA clone IMAGE:5060660 tumor, gross tissue 5' read
  BI249198 cDNA clone IMAGE:5150820 tumor, gross tissue 5' read
  BF167348 cDNA clone IMAGE:3994352 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BG871529 cDNA clone IMAGE:4921432 salivary gland 5' read
  BI110992 cDNA clone IMAGE:5025622 tumor, gross tissue 5' read Protein similarity
  AW211612 cDNA clone IMAGE:2649222 tumor, gross tissue 5' read
  BI106442 cDNA clone IMAGE:5038009 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  AA895630 cDNA clone IMAGE:1278853 macrophage 5' read Protein similarity
  BG800467 cDNA clone (no-name) neural retina
  AA510616 cDNA clone IMAGE:863499 mammary gland 5' read
  BE381331 cDNA clone IMAGE:3600579 tumor, biopsy sample 5' read Protein similarity
  BI658912 cDNA clone IMAGE:5341791 tumor, gross tissue 5' read Protein similarity
  BF165256 cDNA clone IMAGE:4019383 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI319993 cDNA clone (no-name) pancreas 5' read Protein similarity
  BF178978 cDNA clone IMAGE:4039102 tumor, gross tissue 5' read Protein similarity
  AI574140 cDNA clone IMAGE:1924605 liver 5' read Protein similarity
  BI319682 cDNA clone (no-name) pancreas 5' read Protein similarity
  AW213108 cDNA clone IMAGE:2582017 infiltrating ductal carcinoma 5' read Protein similarity
  BE226723 cDNA clone IMAGE:3256696 tumor, gross tissue 5' read Protein similarity

SENSE ESTs

  BF321085 cDNA clone IMAGE:3673399 infiltrating ductal carcinoma 5' read Protein similarity
  BI988990 cDNA clone (no-name) neural retina
  BI988755 cDNA clone (no-name) neural retina
  AL022854 cDNA clone 528-3N22 dissected endoderm 5' read Protein similarity
  BG081585 cDNA clone H3066H01 5' read Protein similarity
  BI697749 cDNA clone IMAGE:5374355 tumor, biopsy sample 5' read Protein similarity
  BG295047 cDNA clone IMAGE:4503908 retina 5' read Protein similarity
  BF321084 cDNA clone IMAGE:3673399 infiltrating ductal carcinoma 3' read Protein similarityPolyadenylation signal
  AU023495 cDNA clone J0433F11 unfertilized egg 3' read Protein similarityPolyadenylation signal
  BG068556 cDNA clone H3066H01 3' read Protein similarityPolyadenylation signal
  AU024154 cDNA clone J0447E03 unfertilized egg 3' read Protein similarityPolyadenylation signal
  AU023232 cDNA clone J0428B03 unfertilized egg 3' read Protein similarityPolyadenylation signal
  AW229258 cDNA clone IMAGE:2598936 infiltrating ductal carcinoma 5' read
  BI684551 cDNA clone IMAGE:5342911 infiltrating ductal carcinoma 5' read
  AW212542 cDNA clone IMAGE:2598936 infiltrating ductal carcinoma 3' read Protein similarity
  AA606944 cDNA clone IMAGE:1005834 embryo 5' read
  AA537162 cDNA clone IMAGE:944965 embryo 5' read