Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.89561
Description:RIKEN cDNA 4930504E06 gene
Best-Of-UniGene (BOU) SequenceAK015696
Genomic Coordiantes Displayed:Bases 1014947 to 1023302 of contig GA_x5J8B7W4YB8
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.89561.jpeg
Best sense EST/protein match:AK015696 matched dbj|BAB29934.1| (AK015696) evidence:NAS~hypothetical protein~putative [Mus (E = 3e-57)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW012798 cDNA clone IMAGE:2159095 kidney 5' read
  BG091384 cDNA clone IMAGE:4000424 spleen 5' read
  BI987442 cDNA clone (no-name) neural retina
  BG695016 cDNA clone IMAGE:4317420 pituitary gland 5' read
  BI990030 cDNA clone (no-name) neural retina
  AA647641 cDNA clone IMAGE:1067512 mammary gland 5' read
  AA710788 cDNA clone IMAGE:1178682 mammary gland 5' read

SENSE ESTs

  AW260808 cDNA clone IMAGE:2331781 kidney 5' read
  AI956243 cDNA clone IMAGE:2136094 kidney 5' read
  AI226879 cDNA clone IMAGE:1907672 kidney 5' read
  AI317657 cDNA clone IMAGE:1920656 kidney 5' read
  BB580876 cDNA clone 8130401M08 embryonic body between diaphragm and neck
  BF167362 cDNA clone IMAGE:3994260 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF788776 cDNA clone IMAGE:4235563 kidney 5' read
  BG804207 cDNA clone (no-name) neural retina
  BI647811 cDNA clone IMAGE:5320143 tumor, gross tissue 5' read
  BI740034 cDNA clone IMAGE:5365432 retina 5' read
  BE285563 cDNA clone IMAGE:3490074 tumor, gross tissue 5' read
  AA691691 cDNA clone IMAGE:1180787 muscle 5' read
  AA265410 cDNA clone IMAGE:716676 pool 5' read
  BI556568 cDNA clone IMAGE:5292346 tumor, gross tissue 5' read
  AA277405 cDNA clone IMAGE:762237 pool 5' read
  AW320251 cDNA clone IMAGE:2609301 infiltrating ductal carcinoma 5' read
  BI104461 cDNA clone IMAGE:5034974 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI149112 cDNA clone IMAGE:5052622 liver 5' read
  BF533338 cDNA clone IMAGE:4211256 liver 5' read
  BI654158 cDNA clone IMAGE:5324722 tumor, gross tissue 5' read