Steady-state population-average RNA quantitation methodology

Microarrays1

~1000 bp hybridization

experiment

control

• R/G ratios

• R, G values

• quality indicators

ORF

1 DeRisi, et.al., Science 278:680-686 (1997) 4 Brenner et al,

2 Lockhart, et.al., Nat Biotech 14:1675-1680 (1996)

3 Velculescu, et.al, Serial Analysis of Gene Expression, Science 270:484-487 (1995)

MPSS4

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