3D structure & chemical genetics
Tabor & Richardson PNAS 1995 92:6339-43 A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides. (Pub)
F to Y (one atom) gives up to a 8000-fold specificity effect, hence dye-terminators feasible (and uniform).
Louvion et al. Gene 1993 131:129-34. Fusion of GAL4-VP16 to a steroid-binding domain provides a tool for gratuitous induction of galactose-responsive genes in yeast. (Pub)
Shakespeare et al. PNAS 2000 97:9373-8. Structure-based design of an osteoclast-selective, nonpeptide src homology 2 inhibitor with in vivo antiresorptive activity. (Pub)