New Genotyping
& haplotyping technologies
de novo sequencing > scanning > selected sequencing > diagnostic methods
- 1-base Fluorescent, isotopic or Mass-spec* primer extension (Pastinen97)
- 30-base extension Pyrosequencing (Ronaghi99)*
- 700-base extension, capillary arrays dideoxy* (Tabor95, Nickerson97, Heiner98)
- Sequencing by hybridization on arrays (Hacia98, Gentalen99)*
- Chemical & enzymatic cleavage: (Cotton98)
Femtoliter scale reactions (105 molecules)
- 20-base restriction/ligation MPSS (Gross 99)
- 30-base fluorescent in situ amplification sequencing (Mitra 1999)
Single molecule methods (not production)
- Fluorescent exonuclease (Davis91)
- Patch clamp current during ss-DNA nanopore transit (Kasianowicz96)
- Electron, STM, optical microscopy (Lagutina96, Lin99)