Applicability of LP & FBA
Stoichiometry is well-known
Limited thermodynamic information is required
- reversibility vs. irreversibility
Experimental knowledge can be incorporated in to the problem formulation
Linear optimization allows the identification of the reaction pathways used to fulfil the goals of the cell if it is operating in an optimal manner.
The relative value of the metabolites can be determined
Flux distribution for the production of a commercial metabolite can be identified. Genetic Engineering candidates