Expression Data Set 13 details

Information Item Value
Dataset Name Hol_taf145_ts: temperature sensitive TAF145 mutant data
Dataset Number 13
Short Description expression levels as affected by temp sensitive mutation of TAF145
Source URL
Reference Holstege FC; Jennings EG; Wyrick JJ; Lee TI; Hengartner
CJ; Green MR; Golub TR; Lander ES; Young RA, Dissecting the regulatory
circuitry of a eukaryotic genome, Cell 1998 Nov 25;95(5):717-28
Strains YSW93 (CY246 genetic background)
MATa, ade2-101, his3Delta200, leu2Delta1, lys2-801, ura3Delta99, GAL2, GAL3, taf145::LEU2 [AMP, CEN, HIS3, taf145-ts2)]
Conditions YPD Medium,
strain grown to an OD of about 0.6 at permissive temp (30 or 24),
harvested after 45 minutes at 37 or 38
Date Added to ExpressDB Feb 9 1999 1:51:06:600PM
Number of Measures on ExpressDB 10 (here to download dataset and view measure details)
Long Description Data Set loaded by Wayne.
wt strain is YSW87 (MATa, ade2-101, his3Delta200, leu2Delta1, lys2-801, ura3D99, GAL2, GAL3, taf145::LEU2 [pSW104(AMP, CEN, TRP1, TAF145)])
Medline Abstract - Genome-wide expression analysis was
used to identify genes whose expression depends on the functions of
key components of the transcription initiation machinery in
yeast. Components of the polymerase II holoenzyme, the general
transcription factor TFIID, and the SAGA chromatin modification
complex were found to have roles in expression of distinct sets of
genes. The results reveal an unanticipated level of regulation which
is superimposed on that due to gene-specific transcription factors, a
novel mechanism for coordinate regulation of specific sets of genes
when cells encounter limiting nutrients, and evidence that the
ultimate targets of signal transduction pathways can be identified
within the initiation apparatus.

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