| Information Item | Value |
|---|---|
| Dataset Name | Rosetta: Multiple MAPK Pathways |
| Dataset Number | 38 |
| Short Description | intensities, ratios, and P values for 56 series involving both wild type and MAPK pathway mutations |
| Source URL | http://www.rii.com/tech/pubs/s287873.htm |
| Reference | Christopher J. Roberts, Bryce Nelson, Matthew J. Marton, et al(2000) Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles, Science 287: 873-880 |
| Strains | All yeast strains were based on the S288c genetic background and were derived from BY4741 (MATa ura3delta0 leu2delta0 his3delta1 met15delta0) or BY4742 (MATalpha ura3delta0 leu2delta0 his3delta1 lys2delta0) |
| Conditions | Detailed protocols for cultures grown in YPD or SC medium are provided in footnote 10 of this paper |
| Date Added to ExpressDB | May 4 2000 11:12:50:920AM |
| Number of Measures on ExpressDB | 168 (here to download dataset and view measure details) |
| Long Description | Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple MAPK pathways. Analysis of the profiles specified by two different MAPKs, Fus3p and Kss1p, revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways. Full reference is: Christopher J. Roberts, Bryce Nelson, Matthew J. Marton, Roland Stoughton, Michael R. Meyer, Holly A. Bennett, Yudong He, Hongyue Dai, Wynn L. Walker, Timothy R. Hughes, Mike Tyers, Charles Boone, and Stephen H. Friend (2000) Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles, Science 287: 873-880 Notes on Greek letters in Measure Names: The EXCEL spreadsheet the authors provided for this data series included many cases of Delta and alpha in its column headings. When the information was dumped into a tab delimited file to be loaded into this database, EXCEL represented each Delta with a 'D' and each alpha with an 'a'. Every 'D' given in strain genotype symbols in these names is really an uppercase Delta, for example, 'bni1D' is actually 'bni1Delta'. Many of the 'a' characters in the measure names are actually alpha characters. In every case where'aF' is given, it is actually alphaF. Other uses of 'a', including 'a-Factor' and 'MATa' really are the letter 'a' and not a replacement for 'alpha' |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University