Information Item | Value |
---|---|
Dataset Name | Yeast A kinase TPK1 mutant data |
Dataset Number | 39 |
Short Description | TPK1 expression profile from study of TPK1 (alternate name for YJL164C), TPK2 (alternate name for YPL203W), and TPK3 (YRL166C) mutants |
Source URL | http://www.wi.mit.edu/young/PKA/ |
Reference | Robertson LS; Causton HC; Young RA; Fink GR,The yeast A kinases differentially regulate iron update and respiratory functions, PNAS USA 2000, 97(11):5984-5988 |
Strains | base strain 10560-2B (MATa ura3-52 his3::hisG leu2::hisG(Sigma1278b)), mutant LRY520 (add tpk1::URA3) |
Conditions | Yeast cultures grown in YPD Medium were harvested during early to mod exponential phase, extracted RNA hybrized to high-density oligonucleotide arrays |
Date Added to ExpressDB | Aug 31 2000 4:18:35:233PM |
Number of Measures on ExpressDB | 9 (here to download dataset and view measure details) |
Long Description | Abstract - Yeast has three A kinase catalytic subunits, which have greater than 75% identity and are encoded by the TPK genes (TPK1, TPK2, and TPK3) [Toda, T., Cameron, S., Sass, P., Zoller, M. & Wigler, M. (1987) Cell 50, 277-287]. Although they are redundant for viability, the three A kinases are not redundant for pseudohyphal growth [Robertson, L. S. & Fink, G. R. (1998) Proc. Natl. Acad. Sci. USA 95, 13783-13787; Pan, X. & Heitman, J. (1999) Mol. Cell. Biol. 19, 4874-4887]; Tpk2, but not Tpk1 or Tpk3, is required for pseudohyphal growth. Genome-wide transcriptional profiling has revealed unique signatures for each of the three A kinases leading to the identification of additional functional diversity among these proteins. Tpk2 negatively regulates genes involved in iron uptake and positively regulates genes involved in trehalose degradation and water homeostasis. Tpk1 is required for the derepression of branched chain amino acid biosynthesis genes that seem to have a second role in the maintenance of iron levels and DNA stability within mitochondria. The fact that TPK2 mutants grow better than wild types on nonfermentable carbon sources and on media deficient in iron supports the unique role of Tpk2 in respiratory growth and carbon source use. |
Please contact Wayne Rindone for more information, or with any questions, comments, or concerns.
Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University