Expression Data Set 39 details

Information Item Value
Dataset Name Yeast A kinase TPK1 mutant data
Dataset Number 39
Short Description TPK1 expression profile from study of TPK1 (alternate name for YJL164C), TPK2 (alternate name for YPL203W),
and TPK3 (YRL166C) mutants
Source URL
Reference Robertson LS; Causton HC; Young RA; Fink GR,The yeast A kinases
differentially regulate iron update and respiratory functions, PNAS USA 2000,
Strains base strain 10560-2B (MATa ura3-52 his3::hisG leu2::hisG(Sigma1278b)), mutant
LRY520 (add tpk1::URA3)
Conditions Yeast cultures grown in YPD Medium were harvested during early to
mod exponential phase, extracted RNA hybrized to high-density oligonucleotide arrays
Date Added to ExpressDB Aug 31 2000 4:18:35:233PM
Number of Measures on ExpressDB 9 (here to download dataset and view measure details)
Long Description Abstract - Yeast has three A kinase catalytic
subunits, which have greater than 75% identity and are encoded by the
TPK genes (TPK1, TPK2, and TPK3) [Toda, T., Cameron, S., Sass, P.,
Zoller, M. & Wigler, M. (1987) Cell 50, 277-287]. Although they are
redundant for viability, the three A kinases are not redundant for
pseudohyphal growth [Robertson, L. S. & Fink, G. R. (1998)
Proc. Natl. Acad. Sci. USA 95, 13783-13787; Pan, X. & Heitman,
J. (1999) Mol. Cell. Biol. 19, 4874-4887]; Tpk2, but not Tpk1 or
Tpk3, is required for pseudohyphal growth. Genome-wide transcriptional
profiling has revealed unique signatures for each of the three A
kinases leading to the identification of additional functional
diversity among these proteins. Tpk2 negatively regulates genes
involved in iron uptake and positively regulates genes involved in
trehalose degradation and water homeostasis. Tpk1 is required for the
derepression of branched chain amino acid biosynthesis genes that seem
to have a second role in the maintenance of iron levels and DNA
stability within mitochondria. The fact that TPK2 mutants grow better
than wild types on nonfermentable carbon sources and on media
deficient in iron supports the unique role of Tpk2 in respiratory
growth and carbon source use.

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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University