Expression Data Set 4 details

Information Item Value
Dataset Name Hol_kin28_ts: temperature sensitive KIN28 mutant data
Dataset Number 4
Short Description expression levels as affected by temp sensitive mutation of KIN28
Source URL
Reference Holstege FC; Jennings EG; Wyrick JJ; Lee TI; Hengartner
CJ; Green MR; Golub TR; Lander ES; Young RA, Dissecting the regulatory
circuitry of a eukaryotic genome, Cell 1998 Nov 25;95(5):717-28
Strains GF2092 (S288C genetic background)
MATalpha, ura3, trp1, leu2, his3, kin28-ts3
Conditions YPD Medium,
strain grown to an OD of about 0.6 at permissive temp (30 or 24),
harvested after 45 minutes at 37 or 38
Date Added to ExpressDB Feb 5 1999 11:54:35:213AM
Number of Measures on ExpressDB 6 (here to download dataset and view measure details)
Long Description Data Set loaded by Wayne.
wt strain is GF1047 (MATalpha, ura3, trp1, leu2, lys2)
Medline Abstract - Genome-wide expression analysis was
used to identify genes whose expression depends on the functions of
key components of the transcription initiation machinery in
yeast. Components of the polymerase II holoenzyme, the general
transcription factor TFIID, and the SAGA chromatin modification
complex were found to have roles in expression of distinct sets of
genes. The results reveal an unanticipated level of regulation which
is superimposed on that due to gene-specific transcription factors, a
novel mechanism for coordinate regulation of specific sets of genes
when cells encounter limiting nutrients, and evidence that the
ultimate targets of signal transduction pathways can be identified
within the initiation apparatus.

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