Expression Data Set 48 details

Information Item Value
Dataset Name Lopez and Baker study of gcr1 mutant
Dataset Number 48
Short Description Data showing which genes have significantly lower expression for gcr1 mutants when
grown on glucose
Source URL
Reference Lopez, MC; Baker, HV;
Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns.;
J Bacteriol. 182(17):4970-4978; (2000)
Strains NONE
Conditions NONE
Date Added to ExpressDB Jan 10 2001 5:03:32:806PM
Number of Measures on ExpressDB 48 (here to download dataset and view measure details)
Long Description Abstract from PubMed: The phenotype of an organism is
the manifestation of its expressed genome. The gcr1 mutant of yeast
grows at near wild-type rates on nonfermentable carbon sources but
exhibits a severe growth defect when grown in the presence of glucose,
even when nonfermentable carbon sources are available. Using DNA
microarrays, the genomic expression patterns of wild-type and gcr1
mutant yeast growing on various media, with and without glucose, were
compared. A total of 53 open reading frames (ORFs) were identified as
GCR1 dependent based on the criterion that their expression was
reduced twofold or greater in mutant versus wild-type cultures grown
in permissive medium consisting of YP supplemented with glycerol and
lactate. The GCR1-dependent genes, so defined, fell into three
classes: (i) glycolytic enzyme genes, (ii) ORFs carried by Ty
elements, and (iii) genes not previously known to be GCR1
dependent. In wild-type cultures, GCR1-dependent genes accounted for
27% of the total hybridization signal, whereas in mutant cultures,
they accounted for 6% of the total. Glucose addition to the growth
medium resulted in a reprogramming of gene expression in both
wild-type and mutant yeasts. In both strains, glycolytic enzyme gene
expression was induced by the addition of glucose, although the
expression of these genes was still impaired in the mutant compared to
the wild type. By contrast, glucose resulted in a strong induction of
Ty-borne genes in the mutant background but did not greatly affect
their already high expression in the wild-type background. Both
strains responded to glucose by repressing the expression of genes
involved in respiration and the metabolism of alternative carbon
sources. Thus, the severe growth inhibition observed in gcr1 mutants
in the presence of glucose is the result of normal signal transduction
pathways and glucose repression mechanisms operating without
sufficient glycolytic enzyme gene expression to support growth via
glycolysis alone.

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