Expression Data Set 5 details

Information Item Value
Dataset Name Der_diaux: diauxic shift data
Dataset Number 5
Short Description expression levels during metabolic shift from fermentation to respiration
Source URL
Reference Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale, Joseph L. DeRisi, Vishwanath R. Iyer, and Patrick O. Brown, Science 1997 October 24; 278: 680-686
Strains DBY7286 (MATa, ura3, GAL2)
Conditions 7 timepoints in diauxic shift (plus 7 identical t=0 datasets)
MATa ura3, GAL2 YPD (EtOH)
t=0,9.5,12,13,15,17,18.5 hr
19,18.7,17.6,14,7.5,0.2,0 g/l Glucose
5.0E6, 1.3E7, 2.2E7, 4.9E7, 1.0E8, 1.9E8, 2.0E8 cells/ml
OD600= 0.14, 0.46, 0.8, 1.8, 3.7, 6.9, 7.3
Date Added to ExpressDB Feb 5 1999 12:12:36:573PM
Number of Measures on ExpressDB 63 (here to download dataset and view measure details)
Long Description Article abstract - DNA microarrays containing virtually
every gene of Saccharomyces cerevisiae were used to carry out a
comprehensive investigation of the temporal program of gene expression
accompanying the metabolic shift from fermentation to respiration. The
expression profiles observed for genes with known metabolic functions
pointed to features of the metabolic reprogramming that occur during
the diauxic shift, and the expression patterns of many previously
uncharacterized genes provided clues to their possible functions. The
same DNA microarrays were also used to identify genes whose expression
was affected by deletion of the transcriptional co-repressor TUP1 or
overexpression of the transcriptional activator YAP1. These results
demonstrate the feasibility and utility of this approach to genomewide
exploration of gene expression patterns.

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