Expression Data Set 65 details

Information Item Value
Dataset Name Genomic Expression Programs in Response to Environmental Changes
Dataset Number 65
Short Description Expression Time Series following treatment with a variety of stresses, including temperature shifts, osmolarity, esposure to peroxide, menadione, diamide, DTT, amino acid starvation, and nitrogen depletion.
Source URL
Reference Gasch, AP; Spellman, PT; Kao, CM; Carmel-Harel, O; Eisen, MB; Storz, G; Botstein, D; Brown, PO; Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes; Mol Biol Cell 11: 4241-4257 (2000)
Strains Strain genotypes are given in Long Description. The strain names are
DBY7286, DBY8768, DBY9434, DBY9435, DBY9439, DBY9440, and DBY9441
Conditions Grown on YPD at 30 C and shaken at 250-300 rpm.
Date Added to ExpressDB Sep 9 2002 12:46:12:156PM
Number of Measures on ExpressDB 173 (here to download dataset and view measure details)
Long Description Abstract from reference: We explored genomic expression
patterns in the yeast Saccharomyces cerevisiae responding to diverse
environmental transitions. DNA microarrays were used to measure
changes in transcript levels over time for almost every yeast gene, as
cells responded to temperature shocks, hydrogen peroxide, the
superoxide-generating drug menadione, the sulfhydryl-oxidizing agent
diamide, the disulfide-reducing agent dithiothreitol, hyper- and
hypo-osmotic shock, amino acid starvation, nitrogen source depletion,
and progression into stationary phase. A large set of genes (~ 900)
showed a similar drastic response to almost all of these environmental
changes. Additional features of the genomic responses were specialized
for specific conditions. Promoter analysis and subsequent
characterization of the responses of mutant strains implicated the
transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating
specific features of the transcriptional response, while the
identification of novel sequence elements provided clues to novel
regulators. Physiological themes in the genomic responses to specific
environmental stresses provided insights into the effects of those
stresses on the cell.

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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University