Data generated by MultiplexSMAssayPerformance execution at 20-May-2013 10:15:23 Total number of protein polonies assumed on array: 5e+08 Numbers of protein (and probe) species evaluated: 500 998 1991 3972 7924 15811 31548 62946 125594 250594 500000 Numbers of protein polonies per protein: 1e+06 501002 251130 125881 63099 31624 15849 7943 3981 1995 1000 Kd values evaluated for specific protein-probe interactions: 1e-08 1e-09 1e-10 *** PCCF calculations for the following parameters *** protein (and probe) species: 500 number protein polonies each protein: 1e+06 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500; n_protein_polonies: 1e+06; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 7.69231e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.10526e-10; P_minus_free: 6.66667e-11; P_tot_free: 2.77193e-10; PR_complex_conc: 6.74793e-11; PX_complex_conc: 5.53277e-11; XR_complex_conc: 5.53277e-11; f_PR_unnorm: 3.79571e-11; f_PX_unnorm: 3.11218e-11; f_P_unnorm: 2.30921e-10; f_XR_unnorm: 3.11218e-11; f_R_unnorm: 1.73026e-10; f_PR: 0.0752896; f_PX: 0.0617315; f_P: 0.458042; f_XR: 0.0617315; f_R: 0.343205; f_P_all: 0.595063; n_PR: 126524; n_PX: 103739; n_P: 769737; n_XR: 103739; n_R: 576754; n_R_all: 807018; rho_R: 3.6293e+08; n_PR_rand: 1952; n_PR_tot: 128476; lambda_R: 4.30409e+08; n_PR_completely_random: 2650.25; PCCF: 48.4767; *** PCCF simulation results *** PCCF: 48.4767 simulation:coefficient_of_variation: 0.00239412 simulation:estimated_pdf: 4.06359e-05 0.000200866 0.000707721 0.00177993 0.00321113 0.00422651 0.00429114 0.00393513 0.00420121 0.00555911 0.00765756 0.00966319 0.0108609 0.0114609 0.0128809 0.0169429 0.0243635 0.0342208 0.045268 0.0574579 0.0720546 0.0906955 0.114115 0.14223 0.176054 0.218824 0.274537 0.34541 0.429721 0.522481 0.619987 0.726302 0.855099 1.02084 1.22286 1.43944 1.64411 1.83047 2.01564 2.21822 2.43743 2.65265 2.83938 2.98594 3.09733 3.18747 3.26517 3.32381 3.34937 3.33621 3.28697 3.20944 3.12025 3.0329 2.93831 2.81486 2.65614 2.47489 2.28281 2.08154 1.87323 1.66617 1.46781 1.2826 1.1145 0.962828 0.820734 0.68585 0.565116 0.464302 0.38182 0.314172 0.258981 0.2133 0.173445 0.137127 0.105013 0.0791774 0.059752 0.0442851 0.0305401 0.0187577 0.0106097 0.00658998 0.00507615 0.00397915 0.00253029 0.00119402 0.000412235 0.000136577 0.000180772 0.000511765 0.00113433 0.00179564 0.00202395 0.00162433 0.000928206 0.000377664 0.00010941 2.2568e-05 simulation:estimated_pdf_x: 47.921 47.9324 47.9439 47.9553 47.9667 47.9782 47.9896 48.0011 48.0125 48.024 48.0354 48.0469 48.0583 48.0698 48.0812 48.0926 48.1041 48.1155 48.127 48.1384 48.1499 48.1613 48.1728 48.1842 48.1956 48.2071 48.2185 48.23 48.2414 48.2529 48.2643 48.2758 48.2872 48.2987 48.3101 48.3215 48.333 48.3444 48.3559 48.3673 48.3788 48.3902 48.4017 48.4131 48.4245 48.436 48.4474 48.4589 48.4703 48.4818 48.4932 48.5047 48.5161 48.5276 48.539 48.5504 48.5619 48.5733 48.5848 48.5962 48.6077 48.6191 48.6306 48.642 48.6534 48.6649 48.6763 48.6878 48.6992 48.7107 48.7221 48.7336 48.745 48.7564 48.7679 48.7793 48.7908 48.8022 48.8137 48.8251 48.8366 48.848 48.8595 48.8709 48.8823 48.8938 48.9052 48.9167 48.9281 48.9396 48.951 48.9625 48.9739 48.9853 48.9968 49.0082 49.0197 49.0311 49.0426 49.054 simulation:max: 48.9951 simulation:mean: 48.4778 simulation:min: 47.9799 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 48.2059 48.2902 48.3302 48.358 48.3808 48.3992 48.416 48.4315 48.4476 48.4623 48.4769 48.492 48.5066 48.5233 48.5399 48.5566 48.5765 48.598 48.6262 48.6696 48.7486 simulation:std: 0.116062 simulation:var_n_PR: 122982 simulation:var_n_PR_rand: 1955.8 *** PCCF calculations for the following parameters *** protein (and probe) species: 998 number protein polonies each protein: 501002 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 998; n_protein_polonies: 501002; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 5.56174e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.17855e-10; P_minus_free: 3.34001e-11; P_tot_free: 1.51255e-10; PR_complex_conc: 1.89249e-11; PX_complex_conc: 3.02207e-11; XR_complex_conc: 3.02207e-11; f_PR_unnorm: 1.06453e-11; f_PX_unnorm: 1.69992e-11; f_P_unnorm: 1.22656e-10; f_XR_unnorm: 1.69992e-11; f_R_unnorm: 8.43651e-11; f_PR: 0.0422994; f_PX: 0.0675468; f_P: 0.487379; f_XR: 0.0675468; f_R: 0.335228; f_P_all: 0.597225; n_PR: 35484.2; n_PX: 56663.9; n_P: 408854; n_XR: 56663.9; n_R: 281217; n_R_all: 373365; rho_R: 1.80203e+08; n_PR_rand: 516.541; n_PR_tot: 36000.7; lambda_R: 1.99128e+08; n_PR_completely_random: 614.296; PCCF: 58.6049; *** PCCF simulation results *** PCCF: 58.6049 simulation:coefficient_of_variation: 0.00484028 simulation:estimated_pdf: 9.38347e-06 3.98273e-05 0.000131491 0.000344709 0.000745201 0.00142675 0.00258639 0.00452137 0.00740839 0.0110656 0.0150298 0.0188481 0.0223147 0.0257288 0.0301573 0.0371278 0.0477228 0.0617438 0.0779303 0.095436 0.115184 0.139253 0.168766 0.202925 0.240374 0.281116 0.326719 0.378636 0.436075 0.495826 0.555454 0.616424 0.682416 0.753776 0.82488 0.88834 0.941808 0.989583 1.03859 1.09416 1.15795 1.22624 1.29058 1.34319 1.38246 1.41111 1.42973 1.43447 1.42037 1.38494 1.32992 1.26216 1.19094 1.12177 1.05412 0.986169 0.919116 0.854865 0.792714 0.730842 0.669355 0.609621 0.551317 0.491833 0.429267 0.365845 0.307185 0.257506 0.216482 0.181059 0.14921 0.121465 0.0994056 0.083103 0.0703367 0.0585183 0.046709 0.0355294 0.0259136 0.0185044 0.0134893 0.0103551 0.00809545 0.00605918 0.00433138 0.00317869 0.00251998 0.00206635 0.0016438 0.00124113 0.000931723 0.000801851 0.000835337 0.000881668 0.000788606 0.000554709 0.000299725 0.00012358 3.88492e-05 9.30012e-06 simulation:estimated_pdf_x: 57.4643 57.4892 57.5141 57.5389 57.5638 57.5887 57.6136 57.6384 57.6633 57.6882 57.7131 57.7379 57.7628 57.7877 57.8126 57.8374 57.8623 57.8872 57.9121 57.9369 57.9618 57.9867 58.0115 58.0364 58.0613 58.0862 58.111 58.1359 58.1608 58.1857 58.2105 58.2354 58.2603 58.2852 58.31 58.3349 58.3598 58.3847 58.4095 58.4344 58.4593 58.4841 58.509 58.5339 58.5588 58.5836 58.6085 58.6334 58.6583 58.6831 58.708 58.7329 58.7578 58.7826 58.8075 58.8324 58.8573 58.8821 58.907 58.9319 58.9567 58.9816 59.0065 59.0314 59.0562 59.0811 59.106 59.1309 59.1557 59.1806 59.2055 59.2304 59.2552 59.2801 59.305 59.3298 59.3547 59.3796 59.4045 59.4293 59.4542 59.4791 59.504 59.5288 59.5537 59.5786 59.6035 59.6283 59.6532 59.6781 59.703 59.7278 59.7527 59.7776 59.8024 59.8273 59.8522 59.8771 59.9019 59.9268 simulation:max: 59.7839 simulation:mean: 58.6047 simulation:min: 57.6072 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 57.9362 58.1352 58.237 58.3085 58.3618 58.4125 58.4581 58.5 58.536 58.5738 58.6083 58.641 58.6774 58.7114 58.7511 58.796 58.8437 58.9023 58.9728 59.0643 59.2606 simulation:std: 0.283663 simulation:var_n_PR: 35395.1 simulation:var_n_PR_rand: 517.953 *** PCCF calculations for the following parameters *** protein (and probe) species: 1991 number protein polonies each protein: 251130 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 1991; n_protein_polonies: 251130; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.58295e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 6.27648e-11; P_minus_free: 1.6742e-11; P_tot_free: 7.95068e-11; PR_complex_conc: 5.05187e-12; PX_complex_conc: 1.58934e-11; XR_complex_conc: 1.58934e-11; f_PR_unnorm: 2.84168e-12; f_PX_unnorm: 8.94002e-12; f_P_unnorm: 6.35573e-11; f_XR_unnorm: 8.94002e-12; f_R_unnorm: 4.15967e-11; f_PR: 0.0225752; f_PX: 0.0710226; f_P: 0.504921; f_XR: 0.0710226; f_R: 0.330459; f_P_all: 0.598519; n_PR: 9472.25; n_PX: 29800.1; n_P: 211858; n_XR: 29800.1; n_R: 138656; n_R_all: 177928; rho_R: 8.98431e+07; n_PR_rand: 133.688; n_PR_tot: 9605.94; lambda_R: 9.4895e+07; n_PR_completely_random: 146.74; PCCF: 65.4624; *** PCCF simulation results *** PCCF: 65.4624 simulation:coefficient_of_variation: 0.00973555 simulation:estimated_pdf: 1.1924e-05 5.22378e-05 0.000179808 0.000483989 0.00104821 0.00189819 0.00304302 0.00456223 0.00654306 0.00885912 0.0111515 0.0131281 0.0148895 0.0169406 0.0198726 0.0241087 0.0299474 0.0376831 0.0474698 0.0591304 0.0722039 0.0862284 0.101097 0.117213 0.135402 0.156519 0.180829 0.207547 0.235173 0.262517 0.289251 0.315449 0.340967 0.366016 0.391992 0.420766 0.452611 0.485592 0.517184 0.545884 0.571062 0.591704 0.606098 0.613779 0.617381 0.620522 0.623215 0.621229 0.61055 0.5912 0.566441 0.539647 0.512253 0.483798 0.453248 0.42036 0.386223 0.352755 0.321597 0.293191 0.266825 0.241643 0.21747 0.194439 0.172242 0.15043 0.129224 0.109323 0.0911074 0.0745138 0.0595826 0.0468151 0.0369358 0.0301755 0.0257827 0.0224521 0.0192526 0.0160266 0.0130511 0.0105267 0.00843198 0.00665 0.00507758 0.00366957 0.00246996 0.00156415 0.000985204 0.000670366 0.000530974 0.000506561 0.000546068 0.000591944 0.00059592 0.000534744 0.000412428 0.000263416 0.000135212 5.4661e-05 1.72053e-05 4.16559e-06 simulation:estimated_pdf_x: 63.0208 63.0753 63.1298 63.1844 63.2389 63.2934 63.3479 63.4025 63.457 63.5115 63.566 63.6206 63.6751 63.7296 63.7841 63.8387 63.8932 63.9477 64.0022 64.0568 64.1113 64.1658 64.2203 64.2749 64.3294 64.3839 64.4384 64.4929 64.5475 64.602 64.6565 64.711 64.7656 64.8201 64.8746 64.9291 64.9837 65.0382 65.0927 65.1472 65.2018 65.2563 65.3108 65.3653 65.4199 65.4744 65.5289 65.5834 65.638 65.6925 65.747 65.8015 65.8561 65.9106 65.9651 66.0196 66.0742 66.1287 66.1832 66.2377 66.2923 66.3468 66.4013 66.4558 66.5104 66.5649 66.6194 66.6739 66.7285 66.783 66.8375 66.892 66.9466 67.0011 67.0556 67.1101 67.1646 67.2192 67.2737 67.3282 67.3827 67.4373 67.4918 67.5463 67.6008 67.6554 67.7099 67.7644 67.8189 67.8735 67.928 67.9825 68.037 68.0916 68.1461 68.2006 68.2551 68.3097 68.3642 68.4187 simulation:max: 68.0995 simulation:mean: 65.4602 simulation:min: 63.3387 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 63.9865 64.4099 64.6317 64.7895 64.9239 65.0346 65.1311 65.2201 65.3075 65.38 65.4645 65.5462 65.6203 65.7037 65.7915 65.8881 65.9933 66.1172 66.2803 66.5093 66.9275 simulation:std: 0.637291 simulation:var_n_PR: 9502.88 simulation:var_n_PR_rand: 135.031 *** PCCF calculations for the following parameters *** protein (and probe) species: 3972 number protein polonies each protein: 125881 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 3972; n_protein_polonies: 125881; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 2.09556e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 3.24781e-11; P_minus_free: 8.39208e-12; P_tot_free: 4.08702e-11; PR_complex_conc: 1.31034e-12; PX_complex_conc: 8.17197e-12; XR_complex_conc: 8.17197e-12; f_PR_unnorm: 7.37066e-13; f_PX_unnorm: 4.59673e-12; f_P_unnorm: 3.24305e-11; f_XR_unnorm: 4.59673e-12; f_R_unnorm: 2.06601e-11; f_PR: 0.0116955; f_PX: 0.0729395; f_P: 0.514598; f_XR: 0.0729395; f_R: 0.327828; f_P_all: 0.599233; n_PR: 2456.88; n_PX: 15322.4; n_P: 108102; n_XR: 15322.4; n_R: 68866.9; n_R_all: 86646.2; rho_R: 4.4901e+07; n_PR_rand: 34.1242; n_PR_tot: 2491.01; lambda_R: 4.62113e+07; n_PR_completely_random: 35.8191; PCCF: 69.5441; *** PCCF simulation results *** PCCF: 69.5441 simulation:coefficient_of_variation: 0.0192604 simulation:estimated_pdf: 1.99903e-06 8.80523e-06 2.88468e-05 7.03191e-05 0.000127599 0.000172422 0.000173575 0.000130226 7.28442e-05 3.03963e-05 9.51999e-06 2.68018e-06 3.51278e-06 1.4899e-05 5.48285e-05 0.000160493 0.000380236 0.000739652 0.00120605 0.0017084 0.00223879 0.00292025 0.00390763 0.00520016 0.00660015 0.00792237 0.00924761 0.0108866 0.0130484 0.0156245 0.0183991 0.0214376 0.025185 0.0302057 0.0368294 0.0449947 0.0544488 0.0651095 0.0770963 0.0902847 0.104028 0.117636 0.131135 0.14516 0.160071 0.175392 0.190077 0.203255 0.215039 0.226759 0.239897 0.254506 0.268726 0.280201 0.287894 0.292318 0.294148 0.293424 0.290338 0.285822 0.280644 0.274568 0.266827 0.256701 0.243523 0.227181 0.208956 0.191145 0.175395 0.161459 0.147617 0.132352 0.115701 0.0990592 0.0838386 0.0706161 0.0594266 0.0502546 0.0429089 0.0368068 0.0312568 0.0259496 0.021078 0.0170402 0.0140998 0.0121485 0.0107112 0.00923958 0.00750268 0.00567676 0.00408678 0.0029062 0.0020821 0.00145923 0.000935107 0.00051195 0.000228322 8.0426e-05 2.20419e-05 4.56599e-06 simulation:estimated_pdf_x: 62.7252 62.8457 62.9663 63.0868 63.2074 63.3279 63.4485 63.569 63.6896 63.8101 63.9307 64.0512 64.1718 64.2924 64.4129 64.5335 64.654 64.7746 64.8951 65.0157 65.1362 65.2568 65.3773 65.4979 65.6184 65.739 65.8596 65.9801 66.1007 66.2212 66.3418 66.4623 66.5829 66.7034 66.824 66.9445 67.0651 67.1857 67.3062 67.4268 67.5473 67.6679 67.7884 67.909 68.0295 68.1501 68.2706 68.3912 68.5117 68.6323 68.7529 68.8734 68.994 69.1145 69.2351 69.3556 69.4762 69.5967 69.7173 69.8378 69.9584 70.0789 70.1995 70.3201 70.4406 70.5612 70.6817 70.8023 70.9228 71.0434 71.1639 71.2845 71.405 71.5256 71.6462 71.7667 71.8873 72.0078 72.1284 72.2489 72.3695 72.49 72.6106 72.7311 72.8517 72.9722 73.0928 73.2134 73.3339 73.4545 73.575 73.6956 73.8161 73.9367 74.0572 74.1778 74.2983 74.4189 74.5394 74.66 simulation:max: 73.981 simulation:mean: 69.5304 simulation:min: 63.3911 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 66.3183 67.3365 67.8066 68.1297 68.3903 68.6265 68.8475 69.0299 69.2005 69.3792 69.545 69.7072 69.8873 70.0643 70.2509 70.4353 70.6488 70.9081 71.2257 71.6986 72.664 simulation:std: 1.33918 simulation:var_n_PR: 2390.57 simulation:var_n_PR_rand: 34.5249 *** PCCF calculations for the following parameters *** protein (and probe) species: 7924 number protein polonies each protein: 63099 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 7924; n_protein_polonies: 63099; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.14626e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.65481e-11; P_minus_free: 4.20663e-12; P_tot_free: 2.07547e-11; PR_complex_conc: 3.3466e-13; PX_complex_conc: 4.15042e-12; XR_complex_conc: 4.15042e-12; f_PR_unnorm: 1.88246e-13; f_PX_unnorm: 2.33461e-12; f_P_unnorm: 1.6407e-11; f_XR_unnorm: 2.33461e-12; f_R_unnorm: 1.03059e-11; f_PR: 0.00596275; f_PX: 0.0739495; f_P: 0.519696; f_XR: 0.0739495; f_R: 0.326442; f_P_all: 0.599609; n_PR: 627.482; n_PX: 7781.98; n_P: 54689.5; n_XR: 7781.98; n_R: 34352.7; n_R_all: 42762.1; rho_R: 2.24718e+07; n_PR_rand: 8.64422; n_PR_tot: 636.127; lambda_R: 2.28065e+07; n_PR_completely_random: 8.86107; PCCF: 71.7889; *** PCCF simulation results *** PCCF: 71.7889 simulation:coefficient_of_variation: 0.0389467 simulation:estimated_pdf: 1.08588e-06 5.60649e-06 2.10795e-05 5.88909e-05 0.000125305 0.000208612 0.00027894 0.00030828 0.000299759 0.000299678 0.000378072 0.000589552 0.000951726 0.00146785 0.00215911 0.00304357 0.00411415 0.00540107 0.00701711 0.00906112 0.0114938 0.0141616 0.0169296 0.0198251 0.0230489 0.0268139 0.0313044 0.0368437 0.0437359 0.0516768 0.059633 0.0667682 0.07336 0.0803495 0.0881966 0.0966037 0.105068 0.113246 0.120828 0.12731 0.132184 0.135437 0.137634 0.139444 0.141115 0.142133 0.141415 0.138259 0.133145 0.127312 0.121621 0.116091 0.110337 0.103918 0.0964364 0.087919 0.0791398 0.0711123 0.0641859 0.0578824 0.0516055 0.0453247 0.0394759 0.0343437 0.0297691 0.0254488 0.0213116 0.0175356 0.0143304 0.011776 0.00980374 0.00824697 0.0069186 0.00569198 0.00453506 0.00349242 0.00264417 0.0020438 0.00165768 0.00137619 0.00110714 0.000842292 0.00062081 0.000461764 0.000347786 0.000252369 0.000168087 0.000108904 8.68462e-05 9.12439e-05 9.95577e-05 0.000101408 0.000101069 9.90598e-05 8.65371e-05 6.10816e-05 3.30974e-05 1.34908e-05 4.10263e-06 9.3078e-07 simulation:estimated_pdf_x: 60.1501 60.409 60.668 60.9269 61.1858 61.4448 61.7037 61.9626 62.2216 62.4805 62.7394 62.9984 63.2573 63.5162 63.7752 64.0341 64.2931 64.552 64.8109 65.0699 65.3288 65.5877 65.8467 66.1056 66.3645 66.6235 66.8824 67.1413 67.4003 67.6592 67.9181 68.1771 68.436 68.695 68.9539 69.2128 69.4718 69.7307 69.9896 70.2486 70.5075 70.7664 71.0254 71.2843 71.5432 71.8022 72.0611 72.32 72.579 72.8379 73.0969 73.3558 73.6147 73.8737 74.1326 74.3915 74.6505 74.9094 75.1683 75.4273 75.6862 75.9451 76.2041 76.463 76.7219 76.9809 77.2398 77.4988 77.7577 78.0166 78.2756 78.5345 78.7934 79.0524 79.3113 79.5702 79.8292 80.0881 80.347 80.606 80.8649 81.1238 81.3828 81.6417 81.9007 82.1596 82.4185 82.6775 82.9364 83.1953 83.4543 83.7132 83.9721 84.2311 84.49 84.7489 85.0079 85.2668 85.5257 85.7847 simulation:max: 84.3558 simulation:mean: 71.7095 simulation:min: 61.4744 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 65.3433 67.1857 68.1392 68.8306 69.3736 69.8232 70.2223 70.5921 70.964 71.3244 71.6919 72.0265 72.3557 72.7391 73.1503 73.5729 74.0424 74.5746 75.322 76.3815 78.4222 simulation:std: 2.79285 simulation:var_n_PR: 620.305 simulation:var_n_PR_rand: 8.61151 *** PCCF calculations for the following parameters *** protein (and probe) species: 15811 number protein polonies each protein: 31624 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 15811; n_protein_polonies: 31624; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 6.02011e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 8.36243e-12; P_minus_free: 2.10824e-12; P_tot_free: 1.04707e-11; PR_complex_conc: 8.47563e-14; PX_complex_conc: 2.094e-12; XR_complex_conc: 2.094e-12; f_PR_unnorm: 4.76754e-14; f_PX_unnorm: 1.17788e-12; f_P_unnorm: 8.26152e-12; f_XR_unnorm: 1.17788e-12; f_R_unnorm: 5.15205e-12; f_PR: 0.00301419; f_PX: 0.074469; f_P: 0.522319; f_XR: 0.074469; f_R: 0.325729; f_P_all: 0.599802; n_PR: 158.92; n_PX: 3926.31; n_P: 27538.8; n_XR: 3926.31; n_R: 17173.7; n_R_all: 21259; rho_R: 1.12534e+07; n_PR_rand: 2.1803; n_PR_tot: 161.101; lambda_R: 1.13381e+07; n_PR_completely_random: 2.20782; PCCF: 72.9682; *** PCCF simulation results *** PCCF: 72.9682 simulation:coefficient_of_variation: 0.0784102 simulation:estimated_pdf: 6.74287e-07 3.88741e-06 1.51462e-05 4.02831e-05 7.3823e-05 9.41218e-05 8.48949e-05 5.87952e-05 4.67969e-05 7.04918e-05 0.000129369 0.000210967 0.000317035 0.000475708 0.000721321 0.0010716 0.00151544 0.00201748 0.00256718 0.00320769 0.00398797 0.00493363 0.00609525 0.00755763 0.00929939 0.0111067 0.0128058 0.0145368 0.0166198 0.0192238 0.0222948 0.025701 0.0293473 0.0331649 0.0370703 0.0410129 0.0450516 0.0491808 0.0530539 0.0561324 0.0582611 0.0599096 0.0616903 0.0637225 0.0656195 0.0670425 0.0680238 0.0686733 0.0688285 0.0681894 0.0666688 0.0644523 0.0618072 0.0589441 0.0559904 0.0529889 0.049917 0.0467533 0.0435218 0.0402461 0.0368728 0.0332965 0.0295047 0.0256998 0.0222239 0.0193241 0.0169996 0.0150711 0.0133444 0.0117002 0.0100913 0.00853234 0.00708664 0.00583536 0.00482243 0.00402286 0.00336247 0.00277559 0.00224395 0.00177851 0.00138537 0.00106025 0.00079895 0.000597346 0.000446101 0.000332352 0.000247361 0.000188449 0.000156243 0.000148812 0.000157043 0.000165688 0.00015992 0.000133995 9.45773e-05 5.5211e-05 2.62943e-05 1.01197e-05 3.11962e-06 7.68188e-07 simulation:estimated_pdf_x: 49.1755 49.6798 50.1841 50.6884 51.1926 51.6969 52.2012 52.7055 53.2098 53.714 54.2183 54.7226 55.2269 55.7311 56.2354 56.7397 57.244 57.7483 58.2525 58.7568 59.2611 59.7654 60.2696 60.7739 61.2782 61.7825 62.2868 62.791 63.2953 63.7996 64.3039 64.8082 65.3124 65.8167 66.321 66.8253 67.3295 67.8338 68.3381 68.8424 69.3467 69.8509 70.3552 70.8595 71.3638 71.8681 72.3723 72.8766 73.3809 73.8852 74.3894 74.8937 75.398 75.9023 76.4066 76.9108 77.4151 77.9194 78.4237 78.928 79.4322 79.9365 80.4408 80.9451 81.4493 81.9536 82.4579 82.9622 83.4665 83.9707 84.475 84.9793 85.4836 85.9878 86.4921 86.9964 87.5007 88.005 88.5092 89.0135 89.5178 90.0221 90.5264 91.0306 91.5349 92.0392 92.5435 93.0477 93.552 94.0563 94.5606 95.0649 95.5691 96.0734 96.5777 97.082 97.5863 98.0905 98.5948 99.0991 simulation:max: 96.0074 simulation:mean: 72.95 simulation:min: 51.5946 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 60.0973 63.6477 65.7087 67.0055 68.1499 68.9596 69.8129 70.6824 71.4384 72.1509 72.9139 73.5947 74.3263 75.0959 75.9134 76.7916 77.775 78.9052 80.2922 82.5737 86.6972 simulation:std: 5.72003 simulation:var_n_PR: 159.43 simulation:var_n_PR_rand: 2.13415 *** PCCF calculations for the following parameters *** protein (and probe) species: 31548 number protein polonies each protein: 15849 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 31548; n_protein_polonies: 15849; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.09138e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 4.20858e-12; P_minus_free: 1.05659e-12; P_tot_free: 5.26517e-12; PR_complex_conc: 2.13778e-14; PX_complex_conc: 1.053e-12; XR_complex_conc: 1.053e-12; f_PR_unnorm: 1.2025e-14; f_PX_unnorm: 5.92313e-13; f_P_unnorm: 4.15032e-12; f_XR_unnorm: 5.92313e-13; f_R_unnorm: 2.57878e-12; f_PR: 0.00151721; f_PX: 0.0747327; f_P: 0.523651; f_XR: 0.0747327; f_R: 0.325367; f_P_all: 0.5999; n_PR: 40.0836; n_PX: 1974.39; n_P: 13834.5; n_XR: 1974.39; n_R: 8595.99; n_R_all: 10610.5; rho_R: 5.63754e+06; n_PR_rand: 0.548779; n_PR_tot: 40.6324; lambda_R: 5.65892e+06; n_PR_completely_random: 0.552257; PCCF: 73.5752; *** PCCF simulation results *** PCCF: 73.5752 simulation:coefficient_of_variation: 0.156444 simulation:estimated_pdf: 4.19808e-07 3.85992e-06 1.66083e-05 3.75843e-05 5.66222e-05 7.73175e-05 0.000101462 0.000125816 0.000172691 0.000252629 0.000334109 0.000401697 0.000493519 0.000658195 0.000911112 0.0012478 0.00167003 0.00217575 0.00275751 0.00342132 0.00415767 0.00493129 0.00573486 0.00661616 0.00765242 0.00891982 0.0104617 0.0122429 0.0141154 0.0158887 0.0175 0.0190834 0.020804 0.0226534 0.0244681 0.02613 0.027671 0.0291763 0.0306385 0.0319404 0.0329639 0.0336809 0.0341226 0.0343148 0.0342806 0.0340716 0.0337425 0.0332983 0.0326901 0.0318608 0.0307906 0.0295108 0.0280936 0.0266217 0.0251508 0.0236844 0.0221894 0.0206363 0.0190298 0.0174068 0.0158111 0.0142763 0.0128309 0.0115094 0.0103426 0.00933237 0.00843707 0.00759425 0.00676027 0.00593278 0.00514161 0.00441865 0.00377835 0.00321979 0.00274092 0.00234596 0.00203757 0.00180275 0.00161094 0.00142831 0.00123666 0.0010381 0.000846711 0.000675612 0.000531543 0.000416055 0.000328452 0.000266059 0.000223154 0.000191346 0.000162539 0.000131809 9.89206e-05 6.70412e-05 4.03007e-05 2.11991e-05 9.64239e-06 3.77543e-06 1.2515e-06 3.51209e-07 simulation:estimated_pdf_x: 31.3345 32.2677 33.201 34.1342 35.0674 36.0006 36.9339 37.8671 38.8003 39.7335 40.6668 41.6 42.5332 43.4664 44.3997 45.3329 46.2661 47.1993 48.1326 49.0658 49.999 50.9322 51.8655 52.7987 53.7319 54.6651 55.5984 56.5316 57.4648 58.398 59.3313 60.2645 61.1977 62.1309 63.0642 63.9974 64.9306 65.8638 66.7971 67.7303 68.6635 69.5968 70.53 71.4632 72.3964 73.3297 74.2629 75.1961 76.1293 77.0626 77.9958 78.929 79.8622 80.7955 81.7287 82.6619 83.5951 84.5284 85.4616 86.3948 87.328 88.2613 89.1945 90.1277 91.0609 91.9942 92.9274 93.8606 94.7938 95.7271 96.6603 97.5935 98.5267 99.46 100.393 101.326 102.26 103.193 104.126 105.059 105.993 106.926 107.859 108.792 109.725 110.659 111.592 112.525 113.458 114.392 115.325 116.258 117.191 118.124 119.058 119.991 120.924 121.857 122.791 123.724 simulation:max: 117.055 simulation:mean: 73.5765 simulation:min: 34.6427 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 48.6698 55.3862 59.0566 61.7099 63.7407 65.6189 67.3321 68.7643 70.2868 71.6987 73.1499 74.6338 76.1091 77.6312 79.2601 81.079 83.0363 85.3849 88.4783 93.3764 102.912 simulation:std: 11.5106 simulation:var_n_PR: 40.1331 simulation:var_n_PR_rand: 0.561712 *** PCCF calculations for the following parameters *** protein (and probe) species: 62946 number protein polonies each protein: 7943 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 62946; n_protein_polonies: 7943; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.56873e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.11374e-12; P_minus_free: 5.29554e-13; P_tot_free: 2.64329e-12; PR_complex_conc: 5.38123e-15; PX_complex_conc: 5.28651e-13; XR_complex_conc: 5.28651e-13; f_PR_unnorm: 3.02694e-15; f_PX_unnorm: 2.97366e-13; f_P_unnorm: 2.0826e-12; f_XR_unnorm: 2.97366e-13; f_R_unnorm: 1.29163e-12; f_PR: 0.000762072; f_PX: 0.0748657; f_P: 0.524322; f_XR: 0.0748657; f_R: 0.325184; f_P_all: 0.59995; n_PR: 10.0894; n_PX: 991.18; n_P: 6941.73; n_XR: 991.18; n_R: 4305.26; n_R_all: 5306.53; rho_R: 2.82477e+06; n_PR_rand: 0.137982; n_PR_tot: 10.2274; lambda_R: 2.83015e+06; n_PR_completely_random: 0.13842; PCCF: 73.8865; *** PCCF simulation results *** PCCF: 73.8865 simulation:coefficient_of_variation: 0.310712 simulation:estimated_pdf: 9.79558e-07 5.9028e-05 1.25378e-07 1.36232e-06 0.000230746 0.000611471 8.58328e-05 0.00016723 0.00135749 0.00180481 0.000641142 0.000953723 0.00299802 0.00360717 0.00230601 0.00286986 0.00521297 0.00581875 0.00498697 0.00607241 0.00852706 0.00922543 0.00860277 0.00930939 0.0110717 0.011903 0.0120739 0.0130865 0.0146312 0.0154138 0.0155416 0.0159764 0.0167302 0.0171332 0.0171008 0.0170565 0.0171031 0.0170284 0.0168096 0.0165905 0.0163493 0.0159261 0.0152917 0.0145707 0.0138617 0.0131675 0.0124669 0.0117616 0.0110508 0.0103235 0.00957836 0.00883199 0.00810099 0.0073948 0.00672427 0.00610404 0.00554247 0.00503307 0.0045602 0.00410887 0.00367037 0.00324299 0.00283166 0.00244665 0.00209953 0.00179781 0.00154166 0.00132509 0.00113938 0.000976751 0.000832704 0.000706157 0.000598031 0.000508938 0.000437904 0.000381899 0.000336734 0.000298041 0.000262469 0.00022825 0.000195214 0.000164216 0.000136411 0.000112578 9.2896e-05 7.69209e-05 6.37672e-05 5.24891e-05 4.23919e-05 3.31582e-05 2.48594e-05 1.77015e-05 1.18856e-05 7.48085e-06 4.38704e-06 2.38541e-06 1.1992e-06 5.5131e-07 2.30554e-07 8.77111e-08 simulation:estimated_pdf_x: 5.79065 7.61485 9.43904 11.2632 13.0874 14.9116 16.7358 18.56 20.3842 22.2084 24.0326 25.8568 27.681 29.5052 31.3294 33.1536 34.9778 36.802 38.6262 40.4504 42.2746 44.0988 45.923 47.7472 49.5714 51.3956 53.2198 55.044 56.8682 58.6924 60.5166 62.3408 64.165 65.9892 67.8134 69.6375 71.4617 73.2859 75.1101 76.9343 78.7585 80.5827 82.4069 84.2311 86.0553 87.8795 89.7037 91.5279 93.3521 95.1763 97.0005 98.8247 100.649 102.473 104.297 106.121 107.946 109.77 111.594 113.418 115.242 117.067 118.891 120.715 122.539 124.363 126.188 128.012 129.836 131.66 133.484 135.309 137.133 138.957 140.781 142.605 144.43 146.254 148.078 149.902 151.726 153.551 155.375 157.199 159.023 160.847 162.672 164.496 166.32 168.144 169.968 171.793 173.617 175.441 177.265 179.089 180.914 182.738 184.562 186.386 simulation:max: 169.368 simulation:mean: 73.8351 simulation:min: 7.28726 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 28.1539 36.5913 43.7293 50.3866 55.7246 57.8141 59.5309 64.6566 65.9916 71.0791 72.514 75.2839 79.1413 80.7368 85.809 87.9034 93.2803 97.7302 103.03 113.605 131.252 simulation:std: 22.9415 simulation:var_n_PR: 9.95351 simulation:var_n_PR_rand: 0.134978 *** PCCF calculations for the following parameters *** protein (and probe) species: 125594 number protein polonies each protein: 3981 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 125594; n_protein_polonies: 3981; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 7.91177e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.0605e-12; P_minus_free: 2.65405e-13; P_tot_free: 1.3259e-12; PR_complex_conc: 1.35313e-15; PX_complex_conc: 2.65178e-13; XR_complex_conc: 2.65178e-13; f_PR_unnorm: 7.61134e-16; f_PX_unnorm: 1.49163e-13; f_P_unnorm: 1.0444e-12; f_XR_unnorm: 1.49163e-13; f_R_unnorm: 6.47137e-13; f_PR: 0.000382359; f_PX: 0.0749326; f_P: 0.52466; f_XR: 0.0749326; f_R: 0.325092; f_P_all: 0.599975; n_PR: 2.53706; n_PX: 497.199; n_P: 3481.26; n_XR: 497.199; n_R: 2157.08; n_R_all: 2656.81; rho_R: 1.41561e+06; n_PR_rand: 0.034679; n_PR_tot: 2.57174; lambda_R: 1.41697e+06; n_PR_completely_random: 0.0347343; PCCF: 74.0404; *** PCCF simulation results *** PCCF: 74.0404 simulation:coefficient_of_variation: 0.619705 simulation:estimated_pdf: 0.00276427 6.16886e-25 6.03452e-13 1.73215e-06 0.000961834 0.00846787 0.0153234 0.0107789 0.00464924 0.00344622 0.00608398 0.00950933 0.0111716 0.0105759 0.00897523 0.00773983 0.00733381 0.00743229 0.00752423 0.00732992 0.00685938 0.00626507 0.00568788 0.00519001 0.00476825 0.00439168 0.00403338 0.00368346 0.0033441 0.00302145 0.00272006 0.00244127 0.00218416 0.00194702 0.00172903 0.00152994 0.001349 0.00118579 0.00103966 0.000909593 0.00079426 0.000692189 0.000601866 0.000521978 0.000451571 0.000389544 0.000334923 0.000286962 0.000245036 0.000208573 0.000177022 0.000149853 0.000126599 0.000106848 9.01319e-05 7.59814e-05 6.40106e-05 5.38879e-05 4.53277e-05 3.80797e-05 3.19413e-05 2.6779e-05 2.24378e-05 1.87924e-05 1.57343e-05 1.3178e-05 1.1051e-05 9.28604e-06 7.82964e-06 6.64073e-06 5.67547e-06 4.89052e-06 4.24982e-06 3.72541e-06 3.29369e-06 2.93509e-06 2.6357e-06 2.37938e-06 2.15463e-06 1.9519e-06 1.76291e-06 1.58375e-06 1.41158e-06 1.24623e-06 1.08849e-06 9.37242e-07 7.94588e-07 6.62799e-07 5.42758e-07 4.36029e-07 3.43831e-07 2.65574e-07 2.00786e-07 1.48116e-07 1.07144e-07 7.57032e-08 5.22122e-08 3.48607e-08 2.28999e-08 1.46505e-08 simulation:estimated_pdf_x: -0.447812 4.26209 8.97199 13.6819 18.3918 23.1017 27.8116 32.5215 37.2314 41.9413 46.6512 51.3611 56.071 60.7809 65.4908 70.2007 74.9106 79.6205 84.3304 89.0403 93.7502 98.4601 103.17 107.88 112.59 117.3 122.01 126.72 131.429 136.139 140.849 145.559 150.269 154.979 159.689 164.399 169.109 173.819 178.528 183.238 187.948 192.658 197.368 202.078 206.788 211.498 216.208 220.918 225.627 230.337 235.047 239.757 244.467 249.177 253.887 258.597 263.307 268.017 272.726 277.436 282.146 286.856 291.566 296.276 300.986 305.696 310.406 315.116 319.825 324.535 329.245 333.955 338.665 343.375 348.085 352.795 357.505 362.215 366.924 371.634 376.344 381.054 385.764 390.474 395.184 399.894 404.604 409.314 414.024 418.733 423.443 428.153 432.863 437.573 442.283 446.993 451.703 456.413 461.123 465.832 simulation:max: 370.845 simulation:mean: 75.0105 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 28.0963 28.6826 29.1584 29.9729 56.1161 56.9871 57.7458 58.4814 59.6557 84.0475 85.6209 86.8209 88.2644 110.963 114.577 117.557 142.235 154.202 203.481 simulation:std: 46.4844 simulation:var_n_PR: 2.56793 simulation:var_n_PR_rand: 0.0346063 *** PCCF calculations for the following parameters *** protein (and probe) species: 250594 number protein polonies each protein: 1995 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 250594; n_protein_polonies: 1995; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.97782e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 5.31786e-13; P_minus_free: 1.33017e-13; P_tot_free: 6.64803e-13; PR_complex_conc: 3.40067e-16; PX_complex_conc: 1.3296e-13; XR_complex_conc: 1.3296e-13; f_PR_unnorm: 1.91288e-16; f_PX_unnorm: 7.47901e-14; f_P_unnorm: 5.23596e-13; f_XR_unnorm: 7.47901e-14; f_R_unnorm: 3.24283e-13; f_PR: 0.000191738; f_PX: 0.0749662; f_P: 0.524829; f_XR: 0.0749662; f_R: 0.325046; f_P_all: 0.599987; n_PR: 0.637543; n_PX: 249.268; n_P: 1745.09; n_XR: 249.268; n_R: 1080.8; n_R_all: 1330.71; rho_R: 709371; n_PR_rand: 0.0087113; n_PR_tot: 0.646254; lambda_R: 709711; n_PR_completely_random: 0.00871827; PCCF: 74.1264; *** PCCF simulation results *** PCCF: 74.1264 simulation:coefficient_of_variation: 1.24155 simulation:estimated_pdf: 0.0639 0.00307048 0.00232214 0.0022233 0.00208323 0.00192157 0.00176025 0.00160889 0.00147072 0.00134564 0.00123414 0.00113424 0.00104469 0.000964302 0.000891984 0.000826761 0.000767783 0.000714307 0.000665693 0.000621383 0.000580896 0.000543813 0.000509769 0.000478446 0.000449567 0.000422888 0.000398195 0.000375297 0.000354027 0.000334238 0.000315797 0.000298586 0.000282501 0.000267446 0.000253338 0.0002401 0.000227663 0.000215966 0.000204952 0.00019457 0.000184774 0.000175522 0.000166775 0.000158498 0.00015066 0.000143229 0.000136181 0.000129489 0.00012313 0.000117085 0.000111332 0.000105855 0.000100637 9.5662e-05 9.09163e-05 8.63864e-05 8.20602e-05 7.79263e-05 7.3974e-05 7.01936e-05 6.65757e-05 6.31118e-05 5.9794e-05 5.66147e-05 5.3567e-05 5.06445e-05 4.78409e-05 4.51506e-05 4.25684e-05 4.00892e-05 3.77085e-05 3.54218e-05 3.32252e-05 3.1115e-05 2.90875e-05 2.71396e-05 2.52683e-05 2.34708e-05 2.17446e-05 2.00874e-05 1.84971e-05 1.69718e-05 1.55099e-05 1.41099e-05 1.27709e-05 1.14917e-05 1.02718e-05 9.11084e-06 8.00883e-06 6.96608e-06 5.98336e-06 5.06189e-06 4.20344e-06 3.35953e-06 2.39501e-06 1.83419e-06 1.33419e-06 8.99046e-07 3.55867e-07 1.79502e-07 simulation:estimated_pdf_x: -1.06958 12.3646 25.7987 39.2329 52.6671 66.1012 79.5354 92.9696 106.404 119.838 133.272 146.706 160.14 173.575 187.009 200.443 213.877 227.311 240.745 254.18 267.614 281.048 294.482 307.916 321.35 334.785 348.219 361.653 375.087 388.521 401.955 415.389 428.824 442.258 455.692 469.126 482.56 495.994 509.429 522.863 536.297 549.731 563.165 576.599 590.034 603.468 616.902 630.336 643.77 657.204 670.639 684.073 697.507 710.941 724.375 737.809 751.244 764.678 778.112 791.546 804.98 818.414 831.849 845.283 858.717 872.151 885.585 899.019 912.454 925.888 939.322 952.756 966.19 979.624 993.059 1006.49 1019.93 1033.36 1046.8 1060.23 1073.66 1087.1 1100.53 1113.97 1127.4 1140.83 1154.27 1167.7 1181.14 1194.57 1208.01 1221.44 1234.87 1248.31 1261.74 1275.18 1288.61 1302.04 1315.48 1328.91 simulation:max: 682.804 simulation:mean: 73.2087 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 108.889 111.671 113.402 114.922 116.445 118.233 121.498 224.973 234.936 349.662 simulation:std: 90.8925 simulation:var_n_PR: 0.620249 simulation:var_n_PR_rand: 0.00764747 *** PCCF calculations for the following parameters *** protein (and probe) species: 500000 number protein polonies each protein: 1000 K_d of targeting probes (M): 1e-08 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500000; n_protein_polonies: 1000; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-08; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.99681e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.66596e-13; P_minus_free: 6.66667e-14; P_tot_free: 3.33262e-13; PR_complex_conc: 8.54439e-17; PX_complex_conc: 6.66523e-14; XR_complex_conc: 6.66523e-14; f_PR_unnorm: 4.80622e-17; f_PX_unnorm: 3.74919e-14; f_P_unnorm: 2.6246e-13; f_XR_unnorm: 3.74919e-14; f_R_unnorm: 1.62513e-13; f_PR: 9.61234e-05; f_PX: 0.0749831; f_P: 0.524915; f_XR: 0.0749831; f_R: 0.325023; f_P_all: 0.599994; n_PR: 0.160207; n_PX: 124.973; n_P: 874.867; n_XR: 124.973; n_R: 541.711; n_R_all: 666.844; rho_R: 355565; n_PR_rand: 0.00218905; n_PR_tot: 0.162396; lambda_R: 355650; n_PR_completely_random: 0.00218992; PCCF: 74.1561; *** PCCF simulation results *** PCCF: 74.1561 simulation:coefficient_of_variation: 2.4732 simulation:estimated_pdf: 0.137863 0.000531132 0.000235137 0.000215466 0.000208431 0.00020045 0.00019134 0.000181796 0.000172052 0.000162987 0.000154336 0.000146173 0.00013852 0.000131369 0.000124698 0.000118477 0.000112674 0.000107258 0.000102199 9.74668e-05 9.30352e-05 8.88798e-05 8.49781e-05 8.13097e-05 7.7856e-05 7.46002e-05 7.15269e-05 6.86223e-05 6.58738e-05 6.32698e-05 6.07998e-05 5.84543e-05 5.62246e-05 5.41027e-05 5.20813e-05 5.01536e-05 4.83135e-05 4.65554e-05 4.48741e-05 4.32647e-05 4.17228e-05 4.02443e-05 3.88254e-05 3.74625e-05 3.61524e-05 3.48921e-05 3.36788e-05 3.25097e-05 3.13824e-05 3.02947e-05 2.92443e-05 2.82294e-05 2.72479e-05 2.62981e-05 2.53784e-05 2.44871e-05 2.36229e-05 2.27843e-05 2.197e-05 2.11789e-05 2.04096e-05 1.96612e-05 1.89325e-05 1.82227e-05 1.75306e-05 1.68555e-05 1.61965e-05 1.55528e-05 1.49235e-05 1.4308e-05 1.37056e-05 1.31156e-05 1.25374e-05 1.19703e-05 1.14137e-05 1.08671e-05 1.033e-05 9.80189e-06 9.28222e-06 8.77058e-06 8.26654e-06 7.7697e-06 7.2797e-06 6.79622e-06 6.31899e-06 5.84777e-06 5.3824e-06 4.92281e-06 4.46902e-06 4.02119e-06 3.57966e-06 3.145e-06 2.71812e-06 2.30039e-06 1.89385e-06 1.50152e-06 1.12794e-06 7.80028e-07 4.47929e-07 1.30086e-07 simulation:estimated_pdf_x: -1.07944 28.4042 57.8879 87.3716 116.855 146.339 175.823 205.306 234.79 264.274 293.757 323.241 352.725 382.208 411.692 441.176 470.659 500.143 529.627 559.11 588.594 618.078 647.561 677.045 706.529 736.012 765.496 794.98 824.463 853.947 883.431 912.914 942.398 971.882 1001.37 1030.85 1060.33 1089.82 1119.3 1148.78 1178.27 1207.75 1237.23 1266.72 1296.2 1325.69 1355.17 1384.65 1414.14 1443.62 1473.1 1502.59 1532.07 1561.55 1591.04 1620.52 1650.01 1679.49 1708.97 1738.46 1767.94 1797.42 1826.91 1856.39 1885.88 1915.36 1944.84 1974.33 2003.81 2033.29 2062.78 2092.26 2121.74 2151.23 2180.71 2210.2 2239.68 2269.16 2298.65 2328.13 2357.61 2387.1 2416.58 2446.07 2475.55 2505.03 2534.52 2564 2593.48 2622.97 2652.45 2681.93 2711.42 2740.9 2770.39 2799.87 2829.35 2858.84 2888.32 2917.8 simulation:max: 1486.15 simulation:mean: 75.0614 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 406.855 448.65 469.504 869.629 simulation:std: 185.641 simulation:var_n_PR: 0.161363 simulation:var_n_PR_rand: 0.0029913 *** PCCF calculations for the following parameters *** protein (and probe) species: 500 number protein polonies each protein: 1e+06 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500; n_protein_polonies: 1e+06; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.17647e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 7.27273e-11; P_minus_free: 6.66667e-11; P_tot_free: 1.39394e-10; PR_complex_conc: 2.32783e-10; PX_complex_conc: 2.7823e-11; XR_complex_conc: 2.7823e-11; f_PR_unnorm: 1.3094e-10; f_PX_unnorm: 1.56505e-11; f_P_unnorm: 1.53409e-10; f_XR_unnorm: 1.56505e-11; f_R_unnorm: 1.98864e-10; f_PR: 0.254493; f_PX: 0.0304179; f_P: 0.298163; f_XR: 0.0304179; f_R: 0.386508; f_P_all: 0.583074; n_PR: 436468; n_PX: 52168.2; n_P: 511364; n_XR: 52168.2; n_R: 662879; n_R_all: 1.15152e+06; rho_R: 3.81358e+08; n_PR_rand: 1323.3; n_PR_tot: 437791; lambda_R: 6.14141e+08; n_PR_completely_random: 3781.59; PCCF: 115.769; *** PCCF simulation results *** PCCF: 115.769 simulation:coefficient_of_variation: 0.00108259 simulation:estimated_pdf: 3.15557e-05 0.00015017 0.00054224 0.00149985 0.00321209 0.00544987 0.00765718 0.00968334 0.012396 0.0173441 0.0260536 0.0395752 0.0580384 0.0804155 0.105696 0.133826 0.165022 0.199674 0.240434 0.292719 0.360977 0.444089 0.535408 0.628814 0.724393 0.825616 0.932189 1.04047 1.15248 1.2792 1.43024 1.60202 1.78155 1.96007 2.13738 2.31378 2.48384 2.6409 2.7856 2.9246 3.05654 3.16251 3.21596 3.20599 3.14913 3.07457 3.00118 2.93184 2.85944 2.77003 2.64931 2.49523 2.32201 2.15027 1.99428 1.85587 1.72472 1.58426 1.42408 1.25005 1.08013 0.929422 0.800671 0.687358 0.582568 0.484512 0.395886 0.320185 0.259146 0.21181 0.174916 0.144923 0.119856 0.099031 0.0818227 0.0673167 0.0543369 0.04185 0.0297321 0.0189041 0.0106652 0.00575568 0.00379579 0.00351869 0.00355367 0.00312234 0.00220843 0.00122963 0.000548772 0.000270543 0.000360377 0.000774413 0.00137876 0.00184205 0.00183259 0.00135655 0.000747146 0.000306181 9.33584e-05 2.11802e-05 simulation:estimated_pdf_x: 115.275 115.286 115.297 115.309 115.32 115.332 115.343 115.354 115.366 115.377 115.388 115.4 115.411 115.423 115.434 115.445 115.457 115.468 115.479 115.491 115.502 115.514 115.525 115.536 115.548 115.559 115.57 115.582 115.593 115.605 115.616 115.627 115.639 115.65 115.661 115.673 115.684 115.696 115.707 115.718 115.73 115.741 115.752 115.764 115.775 115.787 115.798 115.809 115.821 115.832 115.843 115.855 115.866 115.878 115.889 115.9 115.912 115.923 115.935 115.946 115.957 115.969 115.98 115.991 116.003 116.014 116.026 116.037 116.048 116.06 116.071 116.082 116.094 116.105 116.117 116.128 116.139 116.151 116.162 116.173 116.185 116.196 116.208 116.219 116.23 116.242 116.253 116.264 116.276 116.287 116.299 116.31 116.321 116.333 116.344 116.355 116.367 116.378 116.39 116.401 simulation:max: 116.338 simulation:mean: 115.77 simulation:min: 115.337 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 115.478 115.561 115.608 115.641 115.665 115.687 115.705 115.723 115.739 115.754 115.768 115.785 115.801 115.819 115.835 115.854 115.876 115.902 115.93 115.975 116.062 simulation:std: 0.125331 simulation:var_n_PR: 434294 simulation:var_n_PR_rand: 1326.46 *** PCCF calculations for the following parameters *** protein (and probe) species: 998 number protein polonies each protein: 501002 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 998; n_protein_polonies: 501002; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.11136e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 5.71919e-11; P_minus_free: 3.34001e-11; P_tot_free: 9.0592e-11; PR_complex_conc: 9.17085e-11; PX_complex_conc: 1.81002e-11; XR_complex_conc: 1.81002e-11; f_PR_unnorm: 5.15861e-11; f_PX_unnorm: 1.01814e-11; f_P_unnorm: 8.85332e-11; f_XR_unnorm: 1.01814e-11; f_R_unnorm: 9.57394e-11; f_PR: 0.201334; f_PX: 0.0397367; f_P: 0.345534; f_XR: 0.0397367; f_R: 0.373659; f_P_all: 0.586604; n_PR: 171954; n_PX: 33938; n_P: 295111; n_XR: 33938; n_R: 319131; n_R_all: 525023; rho_R: 1.88304e+08; n_PR_rand: 381.527; n_PR_tot: 172335; lambda_R: 2.80012e+08; n_PR_completely_random: 863.818; PCCF: 199.504; *** PCCF simulation results *** PCCF: 199.504 simulation:coefficient_of_variation: 0.00177786 simulation:estimated_pdf: 7.36569e-06 2.97542e-05 9.32069e-05 0.000226767 0.000433671 0.000671534 0.000898642 0.00116225 0.00159044 0.00225805 0.00308784 0.00390073 0.00457572 0.00518273 0.00597291 0.00719318 0.00896255 0.0113898 0.0147301 0.019391 0.0257511 0.0340627 0.0445709 0.0574765 0.0724245 0.0882596 0.104243 0.121724 0.143734 0.172299 0.20669 0.244266 0.2825 0.320416 0.359283 0.40211 0.451503 0.50747 0.567154 0.626902 0.684708 0.740293 0.792806 0.840326 0.882606 0.921919 0.959947 0.995468 1.02618 1.05112 1.07085 1.0861 1.09636 1.09952 1.09283 1.07495 1.04749 1.01418 0.978311 0.940464 0.898214 0.847936 0.78794 0.720177 0.648879 0.578338 0.512557 0.454821 0.405838 0.36341 0.32505 0.289705 0.256619 0.224345 0.191999 0.160673 0.132778 0.109965 0.0919041 0.0769243 0.0636283 0.0517785 0.0418343 0.0338878 0.0273249 0.021389 0.0158924 0.0111962 0.00765046 0.00519273 0.00349678 0.0023151 0.00154347 0.00106574 0.000730444 0.000450237 0.000230145 9.36655e-05 2.97093e-05 7.36139e-06 simulation:estimated_pdf_x: 197.922 197.952 197.983 198.014 198.044 198.075 198.105 198.136 198.167 198.197 198.228 198.258 198.289 198.32 198.35 198.381 198.411 198.442 198.473 198.503 198.534 198.564 198.595 198.625 198.656 198.687 198.717 198.748 198.778 198.809 198.84 198.87 198.901 198.931 198.962 198.993 199.023 199.054 199.084 199.115 199.145 199.176 199.207 199.237 199.268 199.298 199.329 199.36 199.39 199.421 199.451 199.482 199.513 199.543 199.574 199.604 199.635 199.666 199.696 199.727 199.757 199.788 199.818 199.849 199.88 199.91 199.941 199.971 200.002 200.033 200.063 200.094 200.124 200.155 200.186 200.216 200.247 200.277 200.308 200.338 200.369 200.4 200.43 200.461 200.491 200.522 200.553 200.583 200.614 200.644 200.675 200.706 200.736 200.767 200.797 200.828 200.858 200.889 200.92 200.95 simulation:max: 200.77 simulation:mean: 199.504 simulation:min: 198.102 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 198.682 198.918 199.05 199.134 199.206 199.261 199.318 199.367 199.415 199.463 199.508 199.553 199.598 199.645 199.693 199.746 199.802 199.868 199.955 200.091 200.33 simulation:std: 0.35469 simulation:var_n_PR: 170309 simulation:var_n_PR_rand: 388.301 *** PCCF calculations for the following parameters *** protein (and probe) species: 1991 number protein polonies each protein: 251130 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 1991; n_protein_polonies: 251130; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.0009e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 4.01083e-11; P_minus_free: 1.6742e-11; P_tot_free: 5.68503e-11; PR_complex_conc: 3.22374e-11; PX_complex_conc: 1.13643e-11; XR_complex_conc: 1.13643e-11; f_PR_unnorm: 1.81335e-11; f_PX_unnorm: 6.39245e-12; f_P_unnorm: 5.0813e-11; f_XR_unnorm: 6.39245e-12; f_R_unnorm: 4.58448e-11; f_PR: 0.142139; f_PX: 0.0501068; f_P: 0.398295; f_XR: 0.0501068; f_R: 0.359352; f_P_all: 0.590541; n_PR: 60445.1; n_PX: 21308.1; n_P: 169377; n_XR: 21308.1; n_R: 152816; n_R_all: 234569; rho_R: 9.28663e+07; n_PR_rand: 109.039; n_PR_tot: 60554.1; lambda_R: 1.25104e+08; n_PR_completely_random: 193.453; PCCF: 313.018; *** PCCF simulation results *** PCCF: 313.018 simulation:coefficient_of_variation: 0.00321999 simulation:estimated_pdf: 2.63879e-06 1.28298e-05 4.43519e-05 0.000109015 0.000190525 0.000236806 0.000209741 0.00013523 7.78282e-05 8.87221e-05 0.000194266 0.000392023 0.000651194 0.000981292 0.00150192 0.0023354 0.00340674 0.00452655 0.0057169 0.00721039 0.00910594 0.0113363 0.0141538 0.0183813 0.0246529 0.0323541 0.0400292 0.0472466 0.0552552 0.0653084 0.0775543 0.0920397 0.10893 0.127252 0.145652 0.164668 0.186044 0.209757 0.233909 0.257444 0.2808 0.303803 0.324925 0.342963 0.357828 0.369289 0.376645 0.380313 0.382065 0.382963 0.382337 0.379185 0.37301 0.362896 0.34713 0.325099 0.299432 0.274677 0.253175 0.233881 0.215018 0.195727 0.17595 0.156321 0.137408 0.119038 0.100804 0.0828572 0.0661276 0.0519468 0.0411906 0.0336102 0.0279611 0.0230315 0.0185552 0.014853 0.0119706 0.00960406 0.00752697 0.00575576 0.00436895 0.00333849 0.00258191 0.00202069 0.00154736 0.00107484 0.000637176 0.000342705 0.000235085 0.000242908 0.000258477 0.000242955 0.00023348 0.000249456 0.000247828 0.000192436 0.000109091 4.4253e-05 1.28076e-05 2.63686e-06 simulation:estimated_pdf_x: 308.281 308.379 308.477 308.576 308.674 308.772 308.87 308.968 309.066 309.164 309.262 309.36 309.458 309.556 309.654 309.753 309.851 309.949 310.047 310.145 310.243 310.341 310.439 310.537 310.635 310.733 310.831 310.93 311.028 311.126 311.224 311.322 311.42 311.518 311.616 311.714 311.812 311.91 312.008 312.107 312.205 312.303 312.401 312.499 312.597 312.695 312.793 312.891 312.989 313.087 313.185 313.284 313.382 313.48 313.578 313.676 313.774 313.872 313.97 314.068 314.166 314.264 314.363 314.461 314.559 314.657 314.755 314.853 314.951 315.049 315.147 315.245 315.343 315.441 315.54 315.638 315.736 315.834 315.932 316.03 316.128 316.226 316.324 316.422 316.52 316.618 316.717 316.815 316.913 317.011 317.109 317.207 317.305 317.403 317.501 317.599 317.697 317.795 317.894 317.992 simulation:max: 317.487 simulation:mean: 313.045 simulation:min: 308.785 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 310.726 311.395 311.757 312.001 312.2 312.365 312.512 312.655 312.783 312.915 313.048 313.174 313.307 313.439 313.574 313.718 313.898 314.094 314.341 314.689 315.397 simulation:std: 1.008 simulation:var_n_PR: 59966.2 simulation:var_n_PR_rand: 106.262 *** PCCF calculations for the following parameters *** protein (and probe) species: 3972 number protein polonies each protein: 125881 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 3972; n_protein_polonies: 125881; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 8.35282e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.51319e-11; P_minus_free: 8.39208e-12; P_tot_free: 3.3524e-11; PR_complex_conc: 1.01253e-11; PX_complex_conc: 6.70312e-12; XR_complex_conc: 6.70312e-12; f_PR_unnorm: 5.6955e-12; f_PX_unnorm: 3.7705e-12; f_P_unnorm: 2.82983e-11; f_XR_unnorm: 3.7705e-12; f_R_unnorm: 2.20375e-11; f_PR: 0.0895908; f_PX: 0.0593104; f_P: 0.445136; f_XR: 0.0593104; f_R: 0.346652; f_P_all: 0.594037; n_PR: 18985; n_PX: 12568.3; n_P: 94327.7; n_XR: 12568.3; n_R: 73458.3; n_R_all: 105012; rho_R: 4.58808e+07; n_PR_rand: 30.1994; n_PR_tot: 19015.2; lambda_R: 5.60062e+07; n_PR_completely_random: 43.4112; PCCF: 438.024; *** PCCF simulation results *** PCCF: 438.024 simulation:coefficient_of_variation: 0.00609491 simulation:estimated_pdf: 9.84324e-07 4.34846e-06 1.42781e-05 3.48479e-05 6.32324e-05 8.53854e-05 8.62942e-05 6.74815e-05 4.81793e-05 4.74076e-05 6.85065e-05 0.000101801 0.000144748 0.00022272 0.000381438 0.0006529 0.00102968 0.00146365 0.00189859 0.00234368 0.00292434 0.00381436 0.00507425 0.00656226 0.00805554 0.00952484 0.0112537 0.0136186 0.0167733 0.0205698 0.0247149 0.0290081 0.0335382 0.0386453 0.0446312 0.0514856 0.0589342 0.0667854 0.0751839 0.0843419 0.0939128 0.10297 0.110907 0.118045 0.125004 0.131818 0.137886 0.142439 0.145012 0.14574 0.145268 0.144266 0.142895 0.140646 0.136913 0.131848 0.126323 0.120936 0.115521 0.109737 0.103599 0.0972621 0.0906468 0.0835072 0.0757644 0.0676966 0.0597405 0.0522009 0.0452162 0.0388514 0.0331102 0.027936 0.0232406 0.0189688 0.0151903 0.0120712 0.00967803 0.00787851 0.00647887 0.0053506 0.00440937 0.00357829 0.00282439 0.00218632 0.00170511 0.00135414 0.00107342 0.000844361 0.000677818 0.000559427 0.000453529 0.000346653 0.000251945 0.000179523 0.000123069 7.50731e-05 3.76588e-05 1.4748e-05 4.40564e-06 9.81897e-07 simulation:estimated_pdf_x: 425.573 425.819 426.064 426.309 426.554 426.8 427.045 427.29 427.535 427.781 428.026 428.271 428.516 428.762 429.007 429.252 429.497 429.743 429.988 430.233 430.478 430.724 430.969 431.214 431.459 431.704 431.95 432.195 432.44 432.685 432.931 433.176 433.421 433.666 433.912 434.157 434.402 434.647 434.893 435.138 435.383 435.628 435.874 436.119 436.364 436.609 436.855 437.1 437.345 437.59 437.835 438.081 438.326 438.571 438.816 439.062 439.307 439.552 439.797 440.043 440.288 440.533 440.778 441.024 441.269 441.514 441.759 442.005 442.25 442.495 442.74 442.986 443.231 443.476 443.721 443.966 444.212 444.457 444.702 444.947 445.193 445.438 445.683 445.928 446.174 446.419 446.664 446.909 447.155 447.4 447.645 447.89 448.136 448.381 448.626 448.871 449.117 449.362 449.607 449.852 simulation:max: 448.498 simulation:mean: 438.035 simulation:min: 426.924 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 431.742 433.667 434.64 435.305 435.774 436.236 436.619 436.975 437.306 437.656 437.987 438.358 438.694 439.045 439.463 439.862 440.336 440.854 441.462 442.409 444.246 simulation:std: 2.66978 simulation:var_n_PR: 18770.7 simulation:var_n_PR_rand: 30.0063 *** PCCF calculations for the following parameters *** protein (and probe) species: 7924 number protein polonies each protein: 63099 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 7924; n_protein_polonies: 63099; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 6.27983e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.4403e-11; P_minus_free: 4.20663e-12; P_tot_free: 1.86096e-11; PR_complex_conc: 2.9087e-12; PX_complex_conc: 3.72146e-12; XR_complex_conc: 3.72146e-12; f_PR_unnorm: 1.63614e-12; f_PX_unnorm: 2.09332e-12; f_P_unnorm: 1.52004e-11; f_XR_unnorm: 2.09332e-12; f_R_unnorm: 1.07081e-11; f_PR: 0.0515625; f_PX: 0.0659703; f_P: 0.479035; f_XR: 0.0659703; f_R: 0.337462; f_P_all: 0.596568; n_PR: 5453.77; n_PX: 6977.68; n_P: 50667.6; n_XR: 6977.68; n_R: 35693.3; n_R_all: 48124.8; rho_R: 2.27579e+07; n_PR_rand: 8.07794; n_PR_tot: 5461.85; lambda_R: 2.56665e+07; n_PR_completely_random: 9.97231; PCCF: 547.701; *** PCCF simulation results *** PCCF: 547.701 simulation:coefficient_of_variation: 0.0123101 simulation:estimated_pdf: 5.07002e-07 2.28344e-06 7.94814e-06 2.16585e-05 4.71587e-05 8.54915e-05 0.000137794 0.000213726 0.000333075 0.000509789 0.000731632 0.000964895 0.00118952 0.00143107 0.00175207 0.00220946 0.00282101 0.00355339 0.0043298 0.00507786 0.00580493 0.0066141 0.00763725 0.00897111 0.0106644 0.0127008 0.0149673 0.0172989 0.0196048 0.0219064 0.0242342 0.0265889 0.029059 0.0318851 0.0352858 0.0391962 0.0432185 0.0468785 0.0499372 0.0524084 0.0543131 0.0555808 0.0562564 0.0566274 0.0570012 0.0574284 0.0577219 0.0576426 0.0570123 0.0557781 0.0540629 0.0520761 0.0498712 0.0472298 0.0439168 0.0400865 0.0363085 0.03312 0.030629 0.028561 0.026534 0.0242415 0.021559 0.0186189 0.0157511 0.0132632 0.0112553 0.00962334 0.00820064 0.00689173 0.00570861 0.00470356 0.00388186 0.00318985 0.00257524 0.00203214 0.00158567 0.00124524 0.000986956 0.000774745 0.000591072 0.000437235 0.000315037 0.000222104 0.000158835 0.0001262 0.00011678 0.000115687 0.000109473 9.29011e-05 7.08099e-05 5.30246e-05 4.44468e-05 4.06466e-05 3.42731e-05 2.36184e-05 1.26713e-05 5.2121e-06 1.63879e-06 3.92428e-07 simulation:estimated_pdf_x: 521.027 521.617 522.206 522.795 523.385 523.974 524.563 525.153 525.742 526.331 526.921 527.51 528.099 528.689 529.278 529.867 530.457 531.046 531.635 532.225 532.814 533.403 533.993 534.582 535.171 535.761 536.35 536.939 537.529 538.118 538.708 539.297 539.886 540.476 541.065 541.654 542.244 542.833 543.422 544.012 544.601 545.19 545.78 546.369 546.958 547.548 548.137 548.726 549.316 549.905 550.494 551.084 551.673 552.262 552.852 553.441 554.03 554.62 555.209 555.798 556.388 556.977 557.566 558.156 558.745 559.334 559.924 560.513 561.103 561.692 562.281 562.871 563.46 564.049 564.639 565.228 565.817 566.407 566.996 567.585 568.175 568.764 569.353 569.943 570.532 571.121 571.711 572.3 572.889 573.479 574.068 574.657 575.247 575.836 576.425 577.015 577.604 578.193 578.783 579.372 simulation:max: 575.984 simulation:mean: 547.634 simulation:min: 524.387 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 531.66 536.526 538.967 540.719 542.105 543.127 544.124 545.048 545.869 546.804 547.658 548.502 549.389 550.227 551.167 552.179 553.202 554.663 556.349 558.634 563.266 simulation:std: 6.74141 simulation:var_n_PR: 5482.74 simulation:var_n_PR_rand: 7.80219 *** PCCF calculations for the following parameters *** protein (and probe) species: 15811 number protein polonies each protein: 31624 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 15811; n_protein_polonies: 31624; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 4.19974e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 7.77711e-12; P_minus_free: 2.10824e-12; P_tot_free: 9.88534e-12; PR_complex_conc: 7.87132e-13; PX_complex_conc: 1.97694e-12; XR_complex_conc: 1.97694e-12; f_PR_unnorm: 4.42762e-13; f_PX_unnorm: 1.11203e-12; f_P_unnorm: 7.93227e-12; f_XR_unnorm: 1.11203e-12; f_R_unnorm: 5.2618e-12; f_PR: 0.0279153; f_PX: 0.0701115; f_P: 0.500115; f_XR: 0.0701115; f_R: 0.331747; f_P_all: 0.598142; n_PR: 1475.89; n_PX: 3706.82; n_P: 26441.3; n_XR: 3706.82; n_R: 17539.6; n_R_all: 22722.3; rho_R: 1.13314e+07; n_PR_rand: 2.10354; n_PR_tot: 1478; lambda_R: 1.21186e+07; n_PR_completely_random: 2.35979; PCCF: 626.326; *** PCCF simulation results *** PCCF: 626.326 simulation:coefficient_of_variation: 0.0246834 simulation:estimated_pdf: 1.77269e-07 7.06715e-07 2.2267e-06 5.65058e-06 1.18124e-05 2.11341e-05 3.38347e-05 5.02855e-05 7.02974e-05 9.31575e-05 0.00012042 0.000159243 0.000219622 0.000306407 0.000415532 0.000540853 0.000683999 0.000853518 0.00105653 0.00129777 0.00158923 0.00195331 0.0024078 0.00294453 0.00352486 0.00410394 0.0046746 0.00528476 0.0059998 0.00684826 0.00781594 0.0088884 0.010073 0.0113741 0.0127515 0.0141101 0.015337 0.0163643 0.0172198 0.0180242 0.0189236 0.0199995 0.0212242 0.0224847 0.0236347 0.024538 0.0251097 0.0253509 0.025349 0.0252203 0.0250306 0.0247655 0.024386 0.0238955 0.0233336 0.0227115 0.0219774 0.0210601 0.0199409 0.0186781 0.0173662 0.016081 0.0148561 0.0136889 0.0125543 0.0114222 0.0102795 0.0091452 0.00806244 0.0070739 0.00620075 0.00544154 0.00478025 0.00419304 0.00365615 0.00315652 0.00269368 0.00226872 0.00187793 0.00152096 0.0012072 0.00094925 0.000754078 0.000620913 0.000540341 0.000490963 0.000446114 0.000388769 0.000318908 0.000247538 0.000185885 0.000138532 0.000102735 7.32181e-05 4.74731e-05 2.66847e-05 1.25518e-05 4.83926e-06 1.51448e-06 3.72619e-07 simulation:estimated_pdf_x: 564.622 565.875 567.129 568.382 569.636 570.889 572.143 573.396 574.65 575.903 577.157 578.41 579.664 580.917 582.171 583.424 584.678 585.931 587.185 588.438 589.692 590.945 592.199 593.452 594.705 595.959 597.212 598.466 599.719 600.973 602.226 603.48 604.733 605.987 607.24 608.494 609.747 611.001 612.254 613.508 614.761 616.015 617.268 618.522 619.775 621.029 622.282 623.536 624.789 626.043 627.296 628.55 629.803 631.057 632.31 633.564 634.817 636.071 637.324 638.578 639.831 641.085 642.338 643.592 644.845 646.099 647.352 648.605 649.859 651.112 652.366 653.619 654.873 656.126 657.38 658.633 659.887 661.14 662.394 663.647 664.901 666.154 667.408 668.661 669.915 671.168 672.422 673.675 674.929 676.182 677.436 678.689 679.943 681.196 682.45 683.703 684.957 686.21 687.464 688.717 simulation:max: 680.9 simulation:mean: 626.276 simulation:min: 572.228 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 591.178 601.021 606.543 610.114 612.981 615.793 618.171 620.342 622.241 624.167 626.155 628.149 630.095 632.209 634.495 636.595 639.17 642.275 646.097 651.868 662.508 simulation:std: 15.4586 simulation:var_n_PR: 1481.94 simulation:var_n_PR_rand: 2.12764 *** PCCF calculations for the following parameters *** protein (and probe) species: 31548 number protein polonies each protein: 15849 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 31548; n_protein_polonies: 15849; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 2.52857e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 4.05499e-12; P_minus_free: 1.05659e-12; P_tot_free: 5.11158e-12; PR_complex_conc: 2.05687e-13; PX_complex_conc: 1.02228e-12; XR_complex_conc: 1.02228e-12; f_PR_unnorm: 1.15699e-13; f_PX_unnorm: 5.75034e-13; f_P_unnorm: 4.06393e-12; f_XR_unnorm: 5.75034e-13; f_R_unnorm: 2.60757e-12; f_PR: 0.0145766; f_PX: 0.0724474; f_P: 0.512006; f_XR: 0.0724474; f_R: 0.328523; f_P_all: 0.59903; n_PR: 385.666; n_PX: 1916.8; n_P: 13546.5; n_XR: 1916.8; n_R: 8691.99; n_R_all: 10994.4; rho_R: 5.65802e+06; n_PR_rand: 0.538733; n_PR_tot: 386.204; lambda_R: 5.86371e+06; n_PR_completely_random: 0.572242; PCCF: 674.896; *** PCCF simulation results *** PCCF: 674.896 simulation:coefficient_of_variation: 0.0496135 simulation:estimated_pdf: 8.82839e-08 5.29709e-07 2.01111e-06 4.87001e-06 7.66589e-06 8.38244e-06 7.81284e-06 8.12584e-06 8.75112e-06 8.3254e-06 7.96547e-06 8.37452e-06 7.88739e-06 6.44287e-06 7.59938e-06 1.52175e-05 3.07018e-05 5.39127e-05 8.50074e-05 0.000124259 0.000173794 0.000240789 0.000332423 0.000448855 0.000586628 0.000744772 0.000925422 0.00113922 0.00141357 0.00176869 0.00218016 0.00260089 0.00302442 0.00348628 0.00402144 0.00466412 0.00544818 0.00634307 0.00723416 0.00802242 0.00869805 0.00930014 0.00986439 0.0103945 0.0108395 0.0111324 0.0112689 0.0113094 0.0113062 0.0112706 0.011204 0.0111097 0.0109538 0.010664 0.0101989 0.00959309 0.00891396 0.00820124 0.00746266 0.00670859 0.00597224 0.00529552 0.00468536 0.00409681 0.00349032 0.00289228 0.00236907 0.00195468 0.00162715 0.00134907 0.00110579 0.000898834 0.000731206 0.000603413 0.000505564 0.000419105 0.000334051 0.00025465 0.000186839 0.000131558 8.92176e-05 6.12157e-05 4.67345e-05 4.15687e-05 3.9906e-05 3.68755e-05 3.0346e-05 2.11241e-05 1.19495e-05 5.41498e-06 2.38802e-06 2.29235e-06 4.04691e-06 6.11495e-06 6.73586e-06 5.33178e-06 3.02722e-06 1.23275e-06 3.60002e-07 7.53818e-08 simulation:estimated_pdf_x: 510.847 514.237 517.628 521.018 524.409 527.8 531.19 534.581 537.971 541.362 544.752 548.143 551.534 554.924 558.315 561.705 565.096 568.486 571.877 575.268 578.658 582.049 585.439 588.83 592.22 595.611 599.002 602.392 605.783 609.173 612.564 615.954 619.345 622.736 626.126 629.517 632.907 636.298 639.689 643.079 646.47 649.86 653.251 656.641 660.032 663.423 666.813 670.204 673.594 676.985 680.375 683.766 687.157 690.547 693.938 697.328 700.719 704.109 707.5 710.891 714.281 717.672 721.062 724.453 727.843 731.234 734.625 738.015 741.406 744.796 748.187 751.578 754.968 758.359 761.749 765.14 768.53 771.921 775.312 778.702 782.093 785.483 788.874 792.264 795.655 799.046 802.436 805.827 809.217 812.608 815.998 819.389 822.78 826.17 829.561 832.951 836.342 839.732 843.123 846.514 simulation:max: 828.87 simulation:mean: 675.009 simulation:min: 526.029 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 598.399 620.155 632.936 640.466 646.568 651.951 656.931 661.477 665.884 670.329 674.782 679.106 683.621 688.178 692.579 697.59 703.113 709.388 717.907 729.494 754.866 simulation:std: 33.4896 simulation:var_n_PR: 388.948 simulation:var_n_PR_rand: 0.541398 *** PCCF calculations for the following parameters *** protein (and probe) species: 62946 number protein polonies each protein: 7943 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 62946; n_protein_polonies: 7943; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.40952e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.07428e-12; P_minus_free: 5.29554e-13; P_tot_free: 2.60383e-12; PR_complex_conc: 5.27336e-14; PX_complex_conc: 5.20759e-13; XR_complex_conc: 5.20759e-13; f_PR_unnorm: 2.96626e-14; f_PX_unnorm: 2.92927e-13; f_P_unnorm: 2.06041e-12; f_XR_unnorm: 2.92927e-13; f_R_unnorm: 1.29903e-12; f_PR: 0.0074624; f_PX: 0.0736932; f_P: 0.518348; f_XR: 0.0736932; f_R: 0.326804; f_P_all: 0.599503; n_PR: 98.8715; n_PX: 976.383; n_P: 6867.74; n_XR: 976.383; n_R: 4329.92; n_R_all: 5405.17; rho_R: 2.83003e+06; n_PR_rand: 0.136691; n_PR_tot: 99.0082; lambda_R: 2.88276e+06; n_PR_completely_random: 0.140993; PCCF: 702.219; *** PCCF simulation results *** PCCF: 702.219 simulation:coefficient_of_variation: 0.0990255 simulation:estimated_pdf: 6.45448e-08 4.91724e-07 2.28919e-06 6.78653e-06 1.36884e-05 2.06332e-05 2.61042e-05 3.16562e-05 4.26528e-05 6.52088e-05 9.78654e-05 0.000130664 0.000155336 0.000173851 0.000199665 0.000249348 0.000328377 0.000426728 0.000534752 0.000661715 0.000826139 0.00102877 0.00125174 0.00148551 0.00173967 0.00202523 0.00234202 0.00268125 0.0030229 0.00334152 0.00363289 0.00391662 0.0042031 0.00448195 0.00474574 0.00499817 0.00523193 0.00541759 0.00552955 0.00557355 0.00557759 0.00556522 0.00553997 0.00548324 0.00536875 0.00518734 0.00495614 0.0047018 0.00444049 0.00417456 0.00389983 0.00361171 0.00330815 0.00299396 0.00268089 0.00237955 0.00209462 0.00183143 0.00160022 0.00140629 0.0012403 0.00108626 0.000937314 0.000798612 0.000676892 0.000572227 0.000479289 0.00039407 0.000317512 0.00025228 0.000197957 0.000151613 0.000111983 8.09218e-05 6.03258e-05 4.87549e-05 4.19207e-05 3.60845e-05 3.00575e-05 2.43053e-05 1.9296e-05 1.50179e-05 1.13459e-05 8.35879e-06 6.2146e-06 4.79843e-06 3.70769e-06 2.64217e-06 1.70889e-06 1.22865e-06 1.38892e-06 2.05593e-06 2.77927e-06 3.0389e-06 2.62335e-06 1.78142e-06 9.50263e-07 3.98109e-07 1.30955e-07 3.38107e-08 simulation:estimated_pdf_x: 438.827 445.275 451.723 458.171 464.619 471.068 477.516 483.964 490.412 496.86 503.308 509.756 516.205 522.653 529.101 535.549 541.997 548.445 554.893 561.342 567.79 574.238 580.686 587.134 593.582 600.03 606.479 612.927 619.375 625.823 632.271 638.719 645.167 651.616 658.064 664.512 670.96 677.408 683.856 690.305 696.753 703.201 709.649 716.097 722.545 728.993 735.442 741.89 748.338 754.786 761.234 767.682 774.13 780.579 787.027 793.475 799.923 806.371 812.819 819.267 825.716 832.164 838.612 845.06 851.508 857.956 864.404 870.853 877.301 883.749 890.197 896.645 903.093 909.541 915.99 922.438 928.886 935.334 941.782 948.23 954.678 961.127 967.575 974.023 980.471 986.919 993.367 999.815 1006.26 1012.71 1019.16 1025.61 1032.06 1038.5 1044.95 1051.4 1057.85 1064.3 1070.74 1077.19 simulation:max: 1037.83 simulation:mean: 702.899 simulation:min: 465.702 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 547.892 591.297 615.102 630.099 643.874 654.882 665.206 674.887 683.644 692.463 701.296 710.449 719.302 728.548 738.353 749.264 761.01 774.858 793.166 821.243 867.857 simulation:std: 69.605 simulation:var_n_PR: 98.96 simulation:var_n_PR_rand: 0.140965 *** PCCF calculations for the following parameters *** protein (and probe) species: 125594 number protein polonies each protein: 3981 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 125594; n_protein_polonies: 3981; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 7.48537e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.05047e-12; P_minus_free: 2.65405e-13; P_tot_free: 1.31588e-12; PR_complex_conc: 1.33845e-14; PX_complex_conc: 2.63173e-13; XR_complex_conc: 2.63173e-13; f_PR_unnorm: 7.52879e-15; f_PX_unnorm: 1.48035e-13; f_P_unnorm: 1.03876e-12; f_XR_unnorm: 1.48035e-13; f_R_unnorm: 6.49017e-13; f_PR: 0.0037807; f_PX: 0.0743379; f_P: 0.52163; f_XR: 0.0743379; f_R: 0.325914; f_P_all: 0.599748; n_PR: 25.0955; n_PX: 493.439; n_P: 3462.47; n_XR: 493.439; n_R: 2163.34; n_R_all: 2681.88; rho_R: 1.41695e+06; n_PR_rand: 0.0345149; n_PR_tot: 25.13; lambda_R: 1.43034e+06; n_PR_completely_random: 0.035062; PCCF: 716.73; *** PCCF simulation results *** PCCF: 716.73 simulation:coefficient_of_variation: 0.198996 simulation:estimated_pdf: 4.68634e-08 8.90474e-07 3.34354e-06 3.32972e-06 3.37074e-06 6.74335e-06 8.80685e-06 1.75522e-05 3.60168e-05 5.4388e-05 7.4907e-05 0.000105151 0.000139858 0.000171438 0.000203569 0.000243916 0.000299343 0.000364471 0.000430815 0.00050092 0.000584189 0.000687349 0.000803438 0.000923999 0.00105757 0.00121761 0.00139862 0.00157696 0.00173518 0.00187693 0.00201698 0.00216406 0.00231373 0.00245047 0.00255653 0.00262564 0.00266686 0.00269182 0.00270345 0.00269927 0.00267887 0.00264565 0.00260434 0.00255714 0.00250193 0.00243446 0.00235292 0.00225947 0.00215774 0.00204873 0.00193038 0.00180092 0.00166174 0.00151662 0.00136992 0.00122659 0.0010928 0.000974122 0.000872267 0.000783578 0.000701466 0.000620631 0.000539757 0.000461102 0.00038849 0.000325379 0.000273594 0.000232914 0.000201419 0.000176413 0.000155222 0.000135652 0.000116089 9.58665e-05 7.55849e-05 5.68439e-05 4.13249e-05 2.98764e-05 2.22262e-05 1.73999e-05 1.42738e-05 1.19575e-05 9.91993e-06 7.95396e-06 6.09347e-06 4.47296e-06 3.24467e-06 2.50615e-06 2.2537e-06 2.35482e-06 2.5695e-06 2.6385e-06 2.41315e-06 1.92298e-06 1.32215e-06 7.8183e-07 3.9681e-07 1.72831e-07 6.45112e-08 2.06211e-08 simulation:estimated_pdf_x: 221.399 233.648 245.897 258.146 270.394 282.643 294.892 307.141 319.39 331.639 343.888 356.137 368.386 380.635 392.884 405.133 417.382 429.631 441.88 454.129 466.378 478.627 490.876 503.125 515.374 527.623 539.872 552.121 564.37 576.619 588.868 601.117 613.366 625.615 637.864 650.113 662.362 674.611 686.86 699.109 711.358 723.607 735.856 748.105 760.354 772.603 784.852 797.101 809.35 821.599 833.848 846.097 858.346 870.595 882.844 895.093 907.342 919.591 931.84 944.089 956.338 968.587 980.835 993.084 1005.33 1017.58 1029.83 1042.08 1054.33 1066.58 1078.83 1091.08 1103.33 1115.57 1127.82 1140.07 1152.32 1164.57 1176.82 1189.07 1201.32 1213.57 1225.82 1238.06 1250.31 1262.56 1274.81 1287.06 1299.31 1311.56 1323.81 1336.06 1348.3 1360.55 1372.8 1385.05 1397.3 1409.55 1421.8 1434.05 simulation:max: 1340.94 simulation:mean: 716.492 simulation:min: 251.465 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 399.095 489.671 538.634 568.706 595.824 618.129 637.958 655.26 675.337 692.736 711.772 729.656 749.693 769.086 789.556 812.648 835.569 863.924 900.056 957.343 1067.98 simulation:std: 142.579 simulation:var_n_PR: 25.263 simulation:var_n_PR_rand: 0.0327944 *** PCCF calculations for the following parameters *** protein (and probe) species: 250594 number protein polonies each protein: 1995 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 250594; n_protein_polonies: 1995; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.86707e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 5.29253e-13; P_minus_free: 1.33017e-13; P_tot_free: 6.6227e-13; PR_complex_conc: 3.37972e-15; PX_complex_conc: 1.32454e-13; XR_complex_conc: 1.32454e-13; f_PR_unnorm: 1.90109e-15; f_PX_unnorm: 7.45051e-14; f_P_unnorm: 5.22172e-13; f_XR_unnorm: 7.45051e-14; f_R_unnorm: 3.24758e-13; f_PR: 0.00190521; f_PX: 0.0746664; f_P: 0.523302; f_XR: 0.0746664; f_R: 0.32546; f_P_all: 0.599873; n_PR: 6.33615; n_PX: 248.318; n_P: 1740.35; n_XR: 248.318; n_R: 1082.38; n_R_all: 1337.04; rho_R: 709708; n_PR_rand: 0.00869055; n_PR_tot: 6.34484; lambda_R: 713088; n_PR_completely_random: 0.00875975; PCCF: 724.318; *** PCCF simulation results *** PCCF: 724.318 simulation:coefficient_of_variation: 0.395601 simulation:estimated_pdf: 0.000102632 1.17055e-76 1.32683e-25 2.86115e-10 0.000131561 0.00031698 4.23477e-06 3.8269e-06 0.000183721 0.000695907 0.000427342 9.51631e-05 0.000257341 0.000814234 0.00103689 0.000668032 0.000483739 0.000826448 0.00123448 0.00123198 0.00101843 0.00104382 0.00130822 0.00147925 0.00142667 0.00133249 0.00135827 0.00144423 0.0014567 0.00137829 0.00129003 0.00124418 0.00122085 0.00118428 0.00113101 0.00107812 0.00103246 0.000985483 0.000928491 0.000862454 0.000794061 0.000728519 0.000667715 0.000612137 0.000561812 0.000515761 0.000471823 0.000427857 0.0003831 0.000338387 0.000295301 0.000255217 0.000218934 0.000186781 0.000158826 0.000134985 0.000114981 9.83567e-05 8.45659e-05 7.30553e-05 6.33393e-05 5.50054e-05 4.77304e-05 4.12812e-05 3.55205e-05 3.03937e-05 2.58899e-05 2.20148e-05 1.87491e-05 1.60473e-05 1.3833e-05 1.20094e-05 1.04716e-05 9.12345e-06 7.88869e-06 6.71825e-06 5.59423e-06 4.52875e-06 3.5487e-06 2.68652e-06 1.9659e-06 1.40035e-06 9.87061e-07 7.13165e-07 5.54868e-07 4.84059e-07 4.67772e-07 4.74974e-07 4.7884e-07 4.62166e-07 4.19114e-07 3.53485e-07 2.76294e-07 1.99545e-07 1.3294e-07 8.17555e-08 4.63313e-08 2.41808e-08 1.16148e-08 5.1305e-09 simulation:estimated_pdf_x: -0.234181 24.5862 49.4066 74.227 99.0474 123.868 148.688 173.509 198.329 223.149 247.97 272.79 297.61 322.431 347.251 372.072 396.892 421.712 446.533 471.353 496.174 520.994 545.814 570.635 595.455 620.275 645.096 669.916 694.737 719.557 744.377 769.198 794.018 818.839 843.659 868.479 893.3 918.12 942.94 967.761 992.581 1017.4 1042.22 1067.04 1091.86 1116.68 1141.5 1166.32 1191.14 1215.96 1240.79 1265.61 1290.43 1315.25 1340.07 1364.89 1389.71 1414.53 1439.35 1464.17 1488.99 1513.81 1538.63 1563.45 1588.27 1613.09 1637.91 1662.73 1687.55 1712.37 1737.19 1762.01 1786.83 1811.65 1836.47 1861.29 1886.12 1910.94 1935.76 1960.58 1985.4 2010.22 2035.04 2059.86 2084.68 2109.5 2134.32 2159.14 2183.96 2208.78 2233.6 2258.42 2283.24 2308.06 2332.88 2357.7 2382.52 2407.34 2432.16 2456.98 simulation:max: 2195.35 simulation:mean: 724.87 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 116.887 321.775 350.19 446.404 465.819 543.365 567.187 584.62 647.213 676.931 695.588 729.26 787.247 812.565 867.792 911.435 956.343 1023.51 1110.85 1219.62 1474.03 simulation:std: 286.76 simulation:var_n_PR: 6.33815 simulation:var_n_PR_rand: 0.00941069 *** PCCF calculations for the following parameters *** protein (and probe) species: 500000 number protein polonies each protein: 1000 K_d of targeting probes (M): 1e-09 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500000; n_protein_polonies: 1000; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-09; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.9685e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.65957e-13; P_minus_free: 6.66667e-14; P_tot_free: 3.32624e-13; PR_complex_conc: 8.51197e-16; PX_complex_conc: 6.65247e-14; XR_complex_conc: 6.65247e-14; f_PR_unnorm: 4.78798e-16; f_PX_unnorm: 3.74201e-14; f_P_unnorm: 2.62101e-13; f_XR_unnorm: 3.74201e-14; f_R_unnorm: 1.62633e-13; f_PR: 0.000957495; f_PX: 0.0748323; f_P: 0.524146; f_XR: 0.0748323; f_R: 0.325231; f_P_all: 0.599936; n_PR: 1.59599; n_PX: 124.734; n_P: 873.67; n_XR: 124.734; n_R: 542.11; n_R_all: 668.44; rho_R: 355650; n_PR_rand: 0.00218643; n_PR_tot: 1.59818; lambda_R: 356501; n_PR_completely_random: 0.00219516; PCCF: 728.046; *** PCCF simulation results *** PCCF: 728.046 simulation:coefficient_of_variation: 0.790972 simulation:estimated_pdf: 0.00783571 1.03784e-37 7.53865e-21 5.47714e-14 1.69707e-10 2.68012e-06 6.44814e-05 0.000402586 0.00107836 0.00160532 0.00156605 0.00112007 0.000645319 0.000350383 0.000255624 0.000305144 0.000430553 0.000568451 0.000669325 0.000707013 0.000681593 0.000612535 0.000526626 0.000446842 0.000386355 0.000348029 0.00032744 0.000316966 0.00030923 0.000299092 0.000284231 0.000264701 0.000242022 0.000218224 0.000195137 0.000174019 0.000155461 0.000139491 0.000125784 0.000113873 0.000103305 9.37316e-05 8.49276e-05 7.67769e-05 6.92361e-05 6.22962e-05 5.59546e-05 5.01961e-05 4.49899e-05 4.02927e-05 3.60537e-05 3.22227e-05 2.87502e-05 2.55989e-05 2.27366e-05 2.01402e-05 1.77925e-05 1.56792e-05 1.37869e-05 1.21011e-05 1.06061e-05 9.28672e-06 8.12484e-06 7.10382e-06 6.20778e-06 5.42008e-06 4.72726e-06 4.11639e-06 3.57732e-06 3.10045e-06 2.67883e-06 2.30582e-06 1.9768e-06 1.68686e-06 1.43231e-06 1.21022e-06 1.0171e-06 8.50399e-07 7.07355e-07 5.85072e-07 4.81246e-07 3.93553e-07 3.19639e-07 2.57823e-07 2.06373e-07 1.64041e-07 1.29129e-07 1.00517e-07 7.75263e-08 5.91168e-08 4.44774e-08 3.29336e-08 2.39852e-08 1.71404e-08 1.20262e-08 8.3111e-09 5.65532e-09 3.76424e-09 2.38667e-09 1.44715e-09 simulation:estimated_pdf_x: -0.454183 46.6517 93.7576 140.864 187.969 235.075 282.181 329.287 376.393 423.499 470.605 517.711 564.817 611.922 659.028 706.134 753.24 800.346 847.452 894.558 941.664 988.77 1035.88 1082.98 1130.09 1177.19 1224.3 1271.41 1318.51 1365.62 1412.72 1459.83 1506.93 1554.04 1601.15 1648.25 1695.36 1742.46 1789.57 1836.68 1883.78 1930.89 1977.99 2025.1 2072.21 2119.31 2166.42 2213.52 2260.63 2307.73 2354.84 2401.95 2449.05 2496.16 2543.26 2590.37 2637.48 2684.58 2731.69 2778.79 2825.9 2873.01 2920.11 2967.22 3014.32 3061.43 3108.54 3155.64 3202.75 3249.85 3296.96 3344.06 3391.17 3438.28 3485.38 3532.49 3579.59 3626.7 3673.81 3720.91 3768.02 3815.12 3862.23 3909.34 3956.44 4003.55 4050.65 4097.76 4144.86 4191.97 4239.08 4286.18 4333.29 4380.39 4427.5 4474.61 4521.71 4568.82 4615.92 4663.03 simulation:max: 3700.19 simulation:mean: 722.801 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 432.38 443.8 452.572 461.845 472.568 488.176 852.569 885.416 908.414 931.616 961.024 1277.89 1355.57 1426.56 1805.98 2343.3 simulation:std: 571.715 simulation:var_n_PR: 1.5649 simulation:var_n_PR_rand: 0.0020958 *** PCCF calculations for the following parameters *** protein (and probe) species: 500 number protein polonies each protein: 1e+06 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500; n_protein_polonies: 1e+06; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.24224e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 9.63855e-12; P_minus_free: 6.66667e-11; P_tot_free: 7.63052e-11; PR_complex_conc: 3.08464e-10; PX_complex_conc: 1.52305e-11; XR_complex_conc: 1.52305e-11; f_PR_unnorm: 1.73511e-10; f_PX_unnorm: 8.56717e-12; f_P_unnorm: 1.17922e-10; f_XR_unnorm: 8.56717e-12; f_R_unnorm: 2.10693e-10; f_PR: 0.334151; f_PX: 0.0164988; f_P: 0.227096; f_XR: 0.0164988; f_R: 0.405756; f_P_all: 0.577745; n_PR: 578370; n_PX: 28557.2; n_P: 393072; n_XR: 28557.2; n_R: 702309; n_R_all: 1.30924e+06; rho_R: 3.89795e+08; n_PR_rand: 1011.98; n_PR_tot: 579382; lambda_R: 6.9826e+08; n_PR_completely_random: 4299.55; PCCF: 134.754; *** PCCF simulation results *** PCCF: 134.754 simulation:coefficient_of_variation: 0.000914708 simulation:estimated_pdf: 2.16727e-05 9.07023e-05 0.000289102 0.000703216 0.00131261 0.0019148 0.00230784 0.00263324 0.00341546 0.00516861 0.00809258 0.0122075 0.0175944 0.0243419 0.0322881 0.0408384 0.0491919 0.0573874 0.0674872 0.0832893 0.108217 0.142812 0.183585 0.225269 0.266275 0.311163 0.366248 0.434321 0.514403 0.60417 0.701107 0.803376 0.912264 1.03357 1.1726 1.32455 1.47573 1.61906 1.76356 1.92236 2.09501 2.26578 2.41916 2.55482 2.68772 2.8326 2.98609 3.12395 3.21893 3.26349 3.27096 3.2554 3.21932 3.16131 3.08406 2.991 2.88462 2.76727 2.63758 2.48754 2.31008 2.10962 1.90268 1.70776 1.53449 1.38189 1.24378 1.11454 0.992358 0.879244 0.777396 0.685132 0.598114 0.514094 0.435147 0.364996 0.304712 0.251647 0.202744 0.158198 0.120945 0.093253 0.0747606 0.0630804 0.0549329 0.0473258 0.0387465 0.0294922 0.0207278 0.0134672 0.00836337 0.0056233 0.0046628 0.00426905 0.003503 0.00230984 0.00118032 0.000462593 0.000138757 3.17915e-05 simulation:estimated_pdf_x: 134.215 134.226 134.236 134.247 134.258 134.268 134.279 134.29 134.3 134.311 134.322 134.332 134.343 134.354 134.365 134.375 134.386 134.397 134.407 134.418 134.429 134.439 134.45 134.461 134.471 134.482 134.493 134.504 134.514 134.525 134.536 134.546 134.557 134.568 134.578 134.589 134.6 134.61 134.621 134.632 134.642 134.653 134.664 134.675 134.685 134.696 134.707 134.717 134.728 134.739 134.749 134.76 134.771 134.781 134.792 134.803 134.813 134.824 134.835 134.846 134.856 134.867 134.878 134.888 134.899 134.91 134.92 134.931 134.942 134.952 134.963 134.974 134.984 134.995 135.006 135.017 135.027 135.038 135.049 135.059 135.07 135.081 135.091 135.102 135.113 135.123 135.134 135.145 135.155 135.166 135.177 135.188 135.198 135.209 135.22 135.23 135.241 135.252 135.262 135.273 simulation:max: 135.212 simulation:mean: 134.754 simulation:min: 134.276 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 134.463 134.549 134.596 134.626 134.651 134.671 134.691 134.708 134.724 134.738 134.754 134.769 134.785 134.8 134.818 134.836 134.855 134.878 134.911 134.958 135.039 simulation:std: 0.12326 simulation:var_n_PR: 581012 simulation:var_n_PR_rand: 1015.85 *** PCCF calculations for the following parameters *** protein (and probe) species: 998 number protein polonies each protein: 501002 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 998; n_protein_polonies: 501002; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.2346e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 9.30362e-12; P_minus_free: 3.34001e-11; P_tot_free: 4.27038e-11; PR_complex_conc: 1.49165e-10; PX_complex_conc: 8.53219e-12; XR_complex_conc: 8.53219e-12; f_PR_unnorm: 8.39052e-11; f_PX_unnorm: 4.79936e-12; f_P_unnorm: 6.1596e-11; f_XR_unnorm: 4.79936e-12; f_R_unnorm: 1.04718e-10; f_PR: 0.322938; f_PX: 0.018472; f_P: 0.237073; f_XR: 0.018472; f_R: 0.403045; f_P_all: 0.578483; n_PR: 279684; n_PX: 15997.9; n_P: 205320; n_XR: 15997.9; n_R: 349062; n_R_all: 644744; rho_R: 1.94698e+08; n_PR_rand: 265.329; n_PR_tot: 279949; lambda_R: 3.43863e+08; n_PR_completely_random: 1060.79; PCCF: 263.905; *** PCCF simulation results *** PCCF: 263.905 simulation:coefficient_of_variation: 0.00132948 simulation:estimated_pdf: 8.90639e-06 3.81392e-05 0.000130602 0.000360916 0.00081625 0.00153207 0.00242587 0.00330546 0.00398686 0.00444551 0.00485524 0.00548475 0.00657368 0.00832181 0.0109467 0.0147375 0.0200955 0.0274949 0.0372613 0.0493149 0.0633023 0.0790779 0.0967987 0.11635 0.137429 0.160824 0.188853 0.223135 0.26218 0.302493 0.3421 0.381916 0.423863 0.468684 0.515799 0.564535 0.615517 0.6706 0.730121 0.790016 0.843776 0.888836 0.929188 0.970275 1.01246 1.05062 1.07914 1.09632 1.10432 1.10625 1.10391 1.09752 1.08583 1.06669 1.03856 1.0016 0.957479 0.908403 0.855586 0.798212 0.735059 0.667886 0.602186 0.543523 0.493452 0.449278 0.407078 0.364726 0.322651 0.282203 0.243677 0.206326 0.170375 0.137965 0.11131 0.0904783 0.0736407 0.0591294 0.0466413 0.0367146 0.029566 0.0246113 0.0209129 0.0178217 0.0151163 0.0127466 0.0106497 0.0087845 0.00718392 0.00586167 0.00471658 0.00363016 0.00259958 0.00171424 0.00103385 0.000557512 0.00025913 9.97079e-05 3.09668e-05 7.4668e-06 simulation:estimated_pdf_x: 262.451 262.481 262.511 262.541 262.571 262.6 262.63 262.66 262.69 262.72 262.75 262.779 262.809 262.839 262.869 262.899 262.928 262.958 262.988 263.018 263.048 263.077 263.107 263.137 263.167 263.197 263.227 263.256 263.286 263.316 263.346 263.376 263.405 263.435 263.465 263.495 263.525 263.554 263.584 263.614 263.644 263.674 263.703 263.733 263.763 263.793 263.823 263.853 263.882 263.912 263.942 263.972 264.002 264.031 264.061 264.091 264.121 264.151 264.18 264.21 264.24 264.27 264.3 264.33 264.359 264.389 264.419 264.449 264.479 264.508 264.538 264.568 264.598 264.628 264.657 264.687 264.717 264.747 264.777 264.806 264.836 264.866 264.896 264.926 264.956 264.985 265.015 265.045 265.075 265.105 265.134 265.164 265.194 265.224 265.254 265.283 265.313 265.343 265.373 265.403 simulation:max: 265.225 simulation:mean: 263.907 simulation:min: 262.629 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 263.1 263.323 263.45 263.537 263.612 263.668 263.725 263.773 263.82 263.864 263.909 263.955 264.001 264.046 264.093 264.145 264.204 264.267 264.358 264.478 264.708 simulation:std: 0.350859 simulation:var_n_PR: 276242 simulation:var_n_PR_rand: 262.956 *** PCCF calculations for the following parameters *** protein (and probe) species: 1991 number protein polonies each protein: 251130 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 1991; n_protein_polonies: 251130; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.21965e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 8.70076e-12; P_minus_free: 1.6742e-11; P_tot_free: 2.54428e-11; PR_complex_conc: 6.99233e-11; PX_complex_conc: 5.086e-12; XR_complex_conc: 5.086e-12; f_PR_unnorm: 3.93318e-11; f_PX_unnorm: 2.86087e-12; f_P_unnorm: 3.31463e-11; f_XR_unnorm: 2.86087e-12; f_R_unnorm: 5.17338e-11; f_PR: 0.302707; f_PX: 0.022018; f_P: 0.255102; f_XR: 0.022018; f_R: 0.398155; f_P_all: 0.579827; n_PR: 131106; n_PX: 9536.24; n_P: 110488; n_XR: 9536.24; n_R: 172446; n_R_all: 313088; rho_R: 9.70571e+07; n_PR_rand: 71.73; n_PR_tot: 131178; lambda_R: 1.6698e+08; n_PR_completely_random: 258.208; PCCF: 508.031; *** PCCF simulation results *** PCCF: 508.031 simulation:coefficient_of_variation: 0.00194646 simulation:estimated_pdf: 2.72539e-06 1.23042e-05 4.14248e-05 0.000105078 0.000204944 0.00031901 0.000418926 0.000489762 0.000517495 0.000481318 0.000392396 0.000333671 0.000440147 0.00082055 0.00146188 0.00219889 0.00284319 0.00339055 0.00406254 0.00512133 0.00668343 0.00867336 0.0109022 0.0132836 0.0161095 0.0200414 0.0256046 0.0325141 0.0397622 0.0468437 0.0546758 0.0646645 0.076999 0.0905441 0.104521 0.119733 0.137662 0.158572 0.181105 0.203716 0.225835 0.247445 0.268304 0.288226 0.307794 0.328263 0.350209 0.372087 0.390538 0.402663 0.407944 0.408011 0.404839 0.399317 0.39129 0.380571 0.367687 0.353586 0.33878 0.323058 0.305969 0.286996 0.265469 0.241006 0.214432 0.187932 0.163931 0.143731 0.127296 0.113864 0.102216 0.0909885 0.0795899 0.0685751 0.0586011 0.0495293 0.0409489 0.0329514 0.0259815 0.0203644 0.0161738 0.0132253 0.0110736 0.00923996 0.00748292 0.00583332 0.00444756 0.0034486 0.00282028 0.00241099 0.00204993 0.00165465 0.00124263 0.000863714 0.000545421 0.000300057 0.000136844 4.95422e-05 1.38233e-05 2.9396e-06 simulation:estimated_pdf_x: 503.369 503.459 503.549 503.639 503.729 503.819 503.909 503.999 504.089 504.18 504.27 504.36 504.45 504.54 504.63 504.72 504.81 504.9 504.991 505.081 505.171 505.261 505.351 505.441 505.531 505.621 505.712 505.802 505.892 505.982 506.072 506.162 506.252 506.342 506.432 506.523 506.613 506.703 506.793 506.883 506.973 507.063 507.153 507.244 507.334 507.424 507.514 507.604 507.694 507.784 507.874 507.964 508.055 508.145 508.235 508.325 508.415 508.505 508.595 508.685 508.775 508.866 508.956 509.046 509.136 509.226 509.316 509.406 509.496 509.587 509.677 509.767 509.857 509.947 510.037 510.127 510.217 510.307 510.398 510.488 510.578 510.668 510.758 510.848 510.938 511.028 511.118 511.209 511.299 511.389 511.479 511.569 511.659 511.749 511.839 511.93 512.02 512.11 512.2 512.29 simulation:max: 511.798 simulation:mean: 508.045 simulation:min: 503.86 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 505.767 506.424 506.798 507.034 507.222 507.385 507.54 507.672 507.792 507.912 508.033 508.159 508.288 508.412 508.551 508.702 508.865 509.052 509.297 509.716 510.371 simulation:std: 0.988889 simulation:var_n_PR: 128297 simulation:var_n_PR_rand: 72.2936 *** PCCF calculations for the following parameters *** protein (and probe) species: 3972 number protein polonies each protein: 125881 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 3972; n_protein_polonies: 125881; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.19087e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 7.70475e-12; P_minus_free: 8.39208e-12; P_tot_free: 1.60968e-11; PR_complex_conc: 3.10371e-11; PX_complex_conc: 3.21856e-12; XR_complex_conc: 3.21856e-12; f_PR_unnorm: 1.74584e-11; f_PX_unnorm: 1.81044e-12; f_P_unnorm: 1.84956e-11; f_XR_unnorm: 1.81044e-12; f_R_unnorm: 2.53051e-11; f_PR: 0.269087; f_PX: 0.0279044; f_P: 0.285074; f_XR: 0.0279044; f_R: 0.39003; f_P_all: 0.582066; n_PR: 58194.4; n_PX: 6034.78; n_P: 61651.8; n_XR: 6034.78; n_R: 84350.2; n_R_all: 148579; rho_R: 4.82053e+07; n_PR_rand: 20.0911; n_PR_tot: 58214.5; lambda_R: 7.92424e+07; n_PR_completely_random: 61.422; PCCF: 947.781; *** PCCF simulation results *** PCCF: 947.781 simulation:coefficient_of_variation: 0.00296824 simulation:estimated_pdf: 9.33144e-07 4.32803e-06 1.45954e-05 3.57927e-05 6.38764e-05 8.32807e-05 8.09829e-05 6.5145e-05 6.0737e-05 8.8641e-05 0.00015893 0.000282261 0.000472059 0.000728214 0.00103847 0.00139046 0.00175201 0.00206204 0.0023028 0.00258346 0.00309545 0.00395058 0.00511213 0.0065493 0.00839556 0.0108109 0.0137432 0.0170098 0.0205306 0.0243327 0.028507 0.0332677 0.0388804 0.045425 0.0527091 0.0604168 0.0683309 0.0764245 0.0847942 0.0933727 0.101712 0.109275 0.115882 0.121641 0.126575 0.130684 0.134176 0.137104 0.138883 0.138865 0.137219 0.134663 0.131566 0.12775 0.122901 0.116976 0.110308 0.103376 0.0964663 0.0895285 0.0823298 0.0747395 0.0668263 0.0587932 0.0509829 0.0438013 0.0374806 0.0319907 0.0272233 0.0231261 0.0196297 0.0165619 0.0137284 0.0110616 0.00865168 0.00663637 0.00507246 0.00390814 0.00304588 0.00239797 0.00188639 0.00145379 0.00107872 0.000760499 0.000504299 0.0003174 0.000195837 0.00011847 6.54687e-05 3.19456e-05 1.99247e-05 2.93627e-05 5.39979e-05 7.80137e-05 8.26094e-05 6.36859e-05 3.5712e-05 1.4566e-05 4.32141e-06 9.32536e-07 simulation:estimated_pdf_x: 934.742 935.01 935.278 935.546 935.814 936.082 936.35 936.618 936.887 937.155 937.423 937.691 937.959 938.227 938.495 938.764 939.032 939.3 939.568 939.836 940.104 940.372 940.64 940.909 941.177 941.445 941.713 941.981 942.249 942.517 942.786 943.054 943.322 943.59 943.858 944.126 944.394 944.662 944.931 945.199 945.467 945.735 946.003 946.271 946.539 946.808 947.076 947.344 947.612 947.88 948.148 948.416 948.684 948.953 949.221 949.489 949.757 950.025 950.293 950.561 950.83 951.098 951.366 951.634 951.902 952.17 952.438 952.706 952.975 953.243 953.511 953.779 954.047 954.315 954.583 954.852 955.12 955.388 955.656 955.924 956.192 956.46 956.728 956.997 957.265 957.533 957.801 958.069 958.337 958.605 958.874 959.142 959.41 959.678 959.946 960.214 960.482 960.75 961.019 961.287 simulation:max: 959.861 simulation:mean: 947.834 simulation:min: 936.166 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 941.324 943.233 944.26 944.942 945.466 945.927 946.337 946.719 947.103 947.471 947.81 948.171 948.549 948.938 949.316 949.743 950.215 950.774 951.436 952.459 954.305 simulation:std: 2.8134 simulation:var_n_PR: 58372.1 simulation:var_n_PR_rand: 20.7256 *** PCCF calculations for the following parameters *** protein (and probe) species: 7924 number protein polonies each protein: 63099 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 7924; n_protein_polonies: 63099; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.13735e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 6.27235e-12; P_minus_free: 4.20663e-12; P_tot_free: 1.0479e-11; PR_complex_conc: 1.26653e-11; PX_complex_conc: 2.09553e-12; XR_complex_conc: 2.09553e-12; f_PR_unnorm: 7.12422e-12; f_PX_unnorm: 1.17874e-12; f_P_unnorm: 1.06269e-11; f_XR_unnorm: 1.17874e-12; f_R_unnorm: 1.22326e-11; f_PR: 0.220283; f_PX: 0.036447; f_P: 0.328587; f_XR: 0.036447; f_R: 0.378236; f_P_all: 0.585318; n_PR: 23747.2; n_PX: 3929.09; n_P: 35422.7; n_XR: 3929.09; n_R: 40774.9; n_R_all: 68451.2; rho_R: 2.38421e+07; n_PR_rand: 5.77718; n_PR_tot: 23753; lambda_R: 3.65073e+07; n_PR_completely_random: 14.1843; PCCF: 1674.6; *** PCCF simulation results *** PCCF: 1674.6 simulation:coefficient_of_variation: 0.00481426 simulation:estimated_pdf: 3.33012e-07 1.52871e-06 5.1739e-06 1.30109e-05 2.50683e-05 3.98798e-05 5.95768e-05 9.24045e-05 0.000143972 0.00020871 0.00027619 0.000342221 0.000411219 0.0004951 0.000618063 0.00081261 0.00109432 0.00144364 0.001822 0.00220904 0.00263303 0.00318876 0.00400648 0.00514073 0.00649189 0.00789918 0.0093046 0.0107769 0.012406 0.0142319 0.0162739 0.0185671 0.021091 0.0236767 0.0261252 0.0284297 0.030775 0.0333268 0.0361265 0.039122 0.042134 0.0448305 0.0469402 0.0484503 0.0494512 0.0499063 0.0497335 0.049018 0.0480007 0.0468793 0.0456716 0.0442612 0.0425324 0.0404315 0.0379716 0.035235 0.0323319 0.0293522 0.026401 0.0236282 0.0211247 0.0188239 0.0166092 0.0145053 0.0126561 0.0110913 0.00965944 0.00823075 0.00684438 0.00561838 0.00459839 0.00374777 0.00302723 0.0024302 0.00195362 0.0015699 0.00124276 0.000964364 0.00075349 0.00061682 0.000526766 0.000442566 0.000342944 0.000237759 0.000153561 0.000105086 8.3717e-05 7.19806e-05 5.93104e-05 4.39852e-05 2.9883e-05 2.27542e-05 2.44241e-05 2.93967e-05 2.95175e-05 2.23332e-05 1.24353e-05 5.07759e-06 1.51762e-06 3.32025e-07 simulation:estimated_pdf_x: 1640.14 1640.88 1641.63 1642.37 1643.12 1643.86 1644.61 1645.35 1646.1 1646.85 1647.59 1648.34 1649.08 1649.83 1650.57 1651.32 1652.06 1652.81 1653.56 1654.3 1655.05 1655.79 1656.54 1657.28 1658.03 1658.77 1659.52 1660.27 1661.01 1661.76 1662.5 1663.25 1663.99 1664.74 1665.48 1666.23 1666.98 1667.72 1668.47 1669.21 1669.96 1670.7 1671.45 1672.19 1672.94 1673.69 1674.43 1675.18 1675.92 1676.67 1677.41 1678.16 1678.91 1679.65 1680.4 1681.14 1681.89 1682.63 1683.38 1684.12 1684.87 1685.62 1686.36 1687.11 1687.85 1688.6 1689.34 1690.09 1690.83 1691.58 1692.33 1693.07 1693.82 1694.56 1695.31 1696.05 1696.8 1697.54 1698.29 1699.04 1699.78 1700.53 1701.27 1702.02 1702.76 1703.51 1704.25 1705 1705.75 1706.49 1707.24 1707.98 1708.73 1709.47 1710.22 1710.96 1711.71 1712.46 1713.2 1713.95 simulation:max: 1709.94 simulation:mean: 1674.55 simulation:min: 1644.13 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 1655.63 1661.22 1664.3 1666.17 1667.81 1669.21 1670.4 1671.44 1672.49 1673.5 1674.47 1675.47 1676.52 1677.62 1678.72 1679.93 1681.24 1682.78 1684.87 1687.92 1693.46 simulation:std: 8.0617 simulation:var_n_PR: 23951.8 simulation:var_n_PR_rand: 5.66414 *** PCCF calculations for the following parameters *** protein (and probe) species: 15811 number protein polonies each protein: 31624 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 15811; n_protein_polonies: 31624; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.04372e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 4.57493e-12; P_minus_free: 2.10824e-12; P_tot_free: 6.68317e-12; PR_complex_conc: 4.6297e-12; PX_complex_conc: 1.33655e-12; XR_complex_conc: 1.33655e-12; f_PR_unnorm: 2.60421e-12; f_PX_unnorm: 7.51809e-13; f_P_unnorm: 6.13105e-12; f_XR_unnorm: 7.51809e-13; f_R_unnorm: 5.86221e-12; f_PR: 0.161741; f_PX: 0.0466931; f_P: 0.380785; f_XR: 0.0466931; f_R: 0.364088; f_P_all: 0.589219; n_PR: 8680.82; n_PX: 2506.07; n_P: 20437.1; n_XR: 2506.07; n_R: 19541; n_R_all: 30727.8; rho_R: 1.17584e+07; n_PR_rand: 1.66115; n_PR_tot: 8682.48; lambda_R: 1.63882e+07; n_PR_completely_random: 3.1912; PCCF: 2720.76; *** PCCF simulation results *** PCCF: 2720.76 simulation:coefficient_of_variation: 0.00823787 simulation:estimated_pdf: 1.16793e-07 4.75857e-07 1.49318e-06 3.61485e-06 6.78478e-06 1.00223e-05 1.2165e-05 1.35576e-05 1.66965e-05 2.50753e-05 4.19296e-05 6.96827e-05 0.000109265 0.000159295 0.000216692 0.00027822 0.000341349 0.000404672 0.000469189 0.000538933 0.000617968 0.000708481 0.000815544 0.000953598 0.00114379 0.00140909 0.00177417 0.00225817 0.00285254 0.00351017 0.00417356 0.00482371 0.00549677 0.00624545 0.00708098 0.00796008 0.00882973 0.00967701 0.010528 0.0114155 0.0123527 0.0133212 0.014259 0.0150705 0.0156789 0.0160833 0.0163555 0.016568 0.0167374 0.0168508 0.0169198 0.0169637 0.0169501 0.0168011 0.0164647 0.0159644 0.0153806 0.0147865 0.0141832 0.0134977 0.0126586 0.011677 0.010637 0.00960933 0.0086021 0.00760618 0.00665619 0.0058234 0.00515399 0.00462755 0.00416965 0.00370461 0.00320265 0.00268678 0.00220426 0.00179232 0.0014617 0.00120059 0.000989223 0.000813681 0.000670844 0.00056156 0.000479654 0.000409113 0.00033461 0.000254611 0.000181249 0.000125863 8.88071e-05 6.31347e-05 4.35092e-05 2.90477e-05 2.00219e-05 1.47909e-05 1.08128e-05 6.92861e-06 3.61312e-06 1.47958e-06 4.7117e-07 1.16285e-07 simulation:estimated_pdf_x: 2623.68 2625.62 2627.57 2629.51 2631.45 2633.39 2635.34 2637.28 2639.22 2641.16 2643.11 2645.05 2646.99 2648.93 2650.88 2652.82 2654.76 2656.7 2658.65 2660.59 2662.53 2664.47 2666.42 2668.36 2670.3 2672.24 2674.19 2676.13 2678.07 2680.01 2681.96 2683.9 2685.84 2687.79 2689.73 2691.67 2693.61 2695.56 2697.5 2699.44 2701.38 2703.33 2705.27 2707.21 2709.15 2711.1 2713.04 2714.98 2716.92 2718.87 2720.81 2722.75 2724.69 2726.64 2728.58 2730.52 2732.46 2734.41 2736.35 2738.29 2740.23 2742.18 2744.12 2746.06 2748 2749.95 2751.89 2753.83 2755.77 2757.72 2759.66 2761.6 2763.54 2765.49 2767.43 2769.37 2771.31 2773.26 2775.2 2777.14 2779.08 2781.03 2782.97 2784.91 2786.85 2788.8 2790.74 2792.68 2794.62 2796.57 2798.51 2800.45 2802.39 2804.34 2806.28 2808.22 2810.16 2812.11 2814.05 2815.99 simulation:max: 2804.56 simulation:mean: 2720.94 simulation:min: 2635.07 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 2668.67 2684.5 2692.25 2697.68 2701.94 2705.72 2708.89 2711.93 2715 2717.89 2720.84 2723.92 2726.65 2729.63 2732.83 2736.25 2739.85 2744.15 2749.5 2758.35 2773.29 simulation:std: 22.4147 simulation:var_n_PR: 8901.77 simulation:var_n_PR_rand: 1.66773 *** PCCF calculations for the following parameters *** protein (and probe) species: 31548 number protein polonies each protein: 15849 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 31548; n_protein_polonies: 15849; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 8.96475e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.9708e-12; P_minus_free: 1.05659e-12; P_tot_free: 4.02739e-12; PR_complex_conc: 1.50671e-12; PX_complex_conc: 8.05452e-13; XR_complex_conc: 8.05452e-13; f_PR_unnorm: 8.47522e-13; f_PX_unnorm: 4.53067e-13; f_P_unnorm: 3.45407e-12; f_XR_unnorm: 4.53067e-13; f_R_unnorm: 2.81086e-12; f_PR: 0.105695; f_PX: 0.0565021; f_P: 0.430758; f_XR: 0.0565021; f_R: 0.350543; f_P_all: 0.592955; n_PR: 2825.1; n_PX: 1510.24; n_P: 11513.7; n_XR: 1510.24; n_R: 9369.61; n_R_all: 13704.9; rho_R: 5.80258e+06; n_PR_rand: 0.465338; n_PR_tot: 2825.56; lambda_R: 7.3093e+06; n_PR_completely_random: 0.713319; PCCF: 3961.15; *** PCCF simulation results *** PCCF: 3961.15 simulation:coefficient_of_variation: 0.0155491 simulation:estimated_pdf: 4.34789e-08 1.92011e-07 6.34113e-07 1.57616e-06 2.98954e-06 4.44788e-06 5.44167e-06 5.81398e-06 5.71318e-06 5.50204e-06 6.05354e-06 8.84458e-06 1.54422e-05 2.66765e-05 4.13132e-05 5.51911e-05 6.37559e-05 6.6985e-05 7.04082e-05 8.05799e-05 0.000101138 0.000134305 0.000182789 0.000246696 0.00032016 0.000396706 0.00047846 0.000576108 0.000698373 0.000844957 0.00101494 0.00121771 0.00146329 0.00174007 0.00201831 0.00228224 0.00254535 0.00282707 0.00312738 0.00343788 0.00376642 0.00413293 0.00453607 0.00493462 0.00527009 0.0055071 0.0056542 0.00575608 0.00586842 0.00602335 0.00620505 0.0063593 0.00643439 0.00641761 0.00633346 0.00620741 0.00603727 0.00580336 0.00549951 0.00514627 0.00477717 0.00441675 0.00406609 0.00370956 0.00334145 0.00298237 0.00266156 0.00238664 0.00213783 0.00188987 0.00163489 0.00138452 0.00115665 0.000963869 0.000810287 0.000691072 0.000593378 0.000503455 0.000416 0.000334924 0.000265693 0.000210575 0.000169213 0.000138871 0.000114315 9.07463e-05 6.73804e-05 4.67807e-05 3.12152e-05 2.07558e-05 1.43375e-05 1.10107e-05 9.66026e-06 8.6738e-06 6.86481e-06 4.38508e-06 2.1776e-06 8.29431e-07 2.40353e-07 5.29712e-08 simulation:estimated_pdf_x: 3676.19 3681.74 3687.29 3692.84 3698.4 3703.95 3709.5 3715.06 3720.61 3726.16 3731.71 3737.27 3742.82 3748.37 3753.92 3759.48 3765.03 3770.58 3776.13 3781.69 3787.24 3792.79 3798.34 3803.9 3809.45 3815 3820.55 3826.11 3831.66 3837.21 3842.76 3848.32 3853.87 3859.42 3864.97 3870.53 3876.08 3881.63 3887.18 3892.74 3898.29 3903.84 3909.39 3914.95 3920.5 3926.05 3931.6 3937.16 3942.71 3948.26 3953.81 3959.37 3964.92 3970.47 3976.02 3981.58 3987.13 3992.68 3998.23 4003.79 4009.34 4014.89 4020.44 4026 4031.55 4037.1 4042.65 4048.21 4053.76 4059.31 4064.86 4070.42 4075.97 4081.52 4087.07 4092.63 4098.18 4103.73 4109.28 4114.84 4120.39 4125.94 4131.5 4137.05 4142.6 4148.15 4153.71 4159.26 4164.81 4170.36 4175.92 4181.47 4187.02 4192.57 4198.13 4203.68 4209.23 4214.78 4220.34 4225.89 simulation:max: 4194.75 simulation:mean: 3961.65 simulation:min: 3706.88 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 3818.87 3861.4 3882.48 3897.25 3909.96 3919.75 3928.92 3937.51 3946.25 3954.63 3962.12 3969.73 3977.53 3985.71 3993.76 4002.71 4012.79 4024.85 4040.67 4062.93 4106.24 simulation:std: 61.6 simulation:var_n_PR: 2859.67 simulation:var_n_PR_rand: 0.472947 *** PCCF calculations for the following parameters *** protein (and probe) species: 62946 number protein polonies each protein: 7943 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 62946; n_protein_polonies: 7943; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 6.99565e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.74796e-12; P_minus_free: 5.29554e-13; P_tot_free: 2.27752e-12; PR_complex_conc: 4.44315e-13; PX_complex_conc: 4.55496e-13; XR_complex_conc: 4.55496e-13; f_PR_unnorm: 2.49927e-13; f_PX_unnorm: 2.56216e-13; f_P_unnorm: 1.87685e-12; f_XR_unnorm: 2.56216e-13; f_R_unnorm: 1.36021e-12; f_PR: 0.0624908; f_PX: 0.0640634; f_P: 0.469281; f_XR: 0.0640634; f_R: 0.340102; f_P_all: 0.595835; n_PR: 833.057; n_PX: 854.021; n_P: 6255.92; n_XR: 854.021; n_R: 4533.86; n_R_all: 6220.94; rho_R: 2.87354e+06; n_PR_rand: 0.125802; n_PR_tot: 833.183; lambda_R: 3.31783e+06; n_PR_completely_random: 0.162273; PCCF: 5134.47; *** PCCF simulation results *** PCCF: 5134.47 simulation:coefficient_of_variation: 0.0308165 simulation:estimated_pdf: 3.47403e-08 1.64673e-07 5.69265e-07 1.44601e-06 2.73227e-06 3.92935e-06 4.50339e-06 4.56942e-06 4.94622e-06 6.43585e-06 9.04084e-06 1.19559e-05 1.45285e-05 1.72094e-05 2.12951e-05 2.78441e-05 3.70481e-05 4.84828e-05 6.20176e-05 7.86083e-05 9.97802e-05 0.000126229 0.000157447 0.0001929 0.000232887 0.000278491 0.000331683 0.000395179 0.000470863 0.000558996 0.000659478 0.000771633 0.00089286 0.00101969 0.00114849 0.00127469 0.00139487 0.00151061 0.00162913 0.00175705 0.0018911 0.00201903 0.00213289 0.00223541 0.00233025 0.00241329 0.00247602 0.00251176 0.00251757 0.00249535 0.00245304 0.00240076 0.00234127 0.00226682 0.00217005 0.00205572 0.00193537 0.00181339 0.00168485 0.00154391 0.00139079 0.00123406 0.00108731 0.000960388 0.0008535 0.000760247 0.000673289 0.000587678 0.000503832 0.000427159 0.000361822 0.000306068 0.000255803 0.000209888 0.000169767 0.000136276 0.000108956 8.69219e-05 6.9128e-05 5.46044e-05 4.27889e-05 3.31139e-05 2.46486e-05 1.67636e-05 9.84801e-06 4.8443e-06 2.18061e-06 1.51348e-06 2.1825e-06 3.50251e-06 4.81357e-06 5.61176e-06 5.62965e-06 4.83016e-06 3.459e-06 2.01094e-06 9.28412e-07 3.35195e-07 9.34004e-08 2.00564e-08 simulation:estimated_pdf_x: 4440.57 4454.99 4469.4 4483.82 4498.24 4512.66 4527.07 4541.49 4555.91 4570.33 4584.74 4599.16 4613.58 4628 4642.42 4656.83 4671.25 4685.67 4700.09 4714.5 4728.92 4743.34 4757.76 4772.17 4786.59 4801.01 4815.43 4829.85 4844.26 4858.68 4873.1 4887.52 4901.93 4916.35 4930.77 4945.19 4959.6 4974.02 4988.44 5002.86 5017.28 5031.69 5046.11 5060.53 5074.95 5089.36 5103.78 5118.2 5132.62 5147.03 5161.45 5175.87 5190.29 5204.71 5219.12 5233.54 5247.96 5262.38 5276.79 5291.21 5305.63 5320.05 5334.47 5348.88 5363.3 5377.72 5392.14 5406.55 5420.97 5435.39 5449.81 5464.22 5478.64 5493.06 5507.48 5521.9 5536.31 5550.73 5565.15 5579.57 5593.98 5608.4 5622.82 5637.24 5651.65 5666.07 5680.49 5694.91 5709.33 5723.74 5738.16 5752.58 5767 5781.41 5795.83 5810.25 5824.67 5839.08 5853.5 5867.92 simulation:max: 5787.47 simulation:mean: 5133.68 simulation:min: 4516.91 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 4765.42 4875.89 4931.56 4969.63 5000.75 5027.4 5050.73 5073.62 5094.12 5114.04 5133.25 5153.2 5173.22 5194.92 5216.35 5240.14 5266.22 5295.74 5335.11 5397.42 5505.16 simulation:std: 158.202 simulation:var_n_PR: 828.587 simulation:var_n_PR_rand: 0.126588 *** PCCF calculations for the following parameters *** protein (and probe) species: 125594 number protein polonies each protein: 3981 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 125594; n_protein_polonies: 3981; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 4.86396e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 9.59734e-13; P_minus_free: 2.65405e-13; P_tot_free: 1.22514e-12; PR_complex_conc: 1.22267e-13; PX_complex_conc: 2.45026e-13; XR_complex_conc: 2.45026e-13; f_PR_unnorm: 6.87752e-14; f_PX_unnorm: 1.37827e-13; f_P_unnorm: 9.87722e-13; f_XR_unnorm: 1.37827e-13; f_R_unnorm: 6.6603e-13; f_PR: 0.0344189; f_PX: 0.0689763; f_P: 0.494311; f_XR: 0.0689763; f_R: 0.333318; f_P_all: 0.597706; n_PR: 229.246; n_PX: 459.414; n_P: 3292.34; n_XR: 459.414; n_R: 2220.05; n_R_all: 2908.71; rho_R: 1.42905e+06; n_PR_rand: 0.0330132; n_PR_tot: 229.279; lambda_R: 1.55131e+06; n_PR_completely_random: 0.0380275; PCCF: 6029.29; *** PCCF simulation results *** PCCF: 6029.29 simulation:coefficient_of_variation: 0.0617481 simulation:estimated_pdf: 9.83628e-09 5.50432e-08 2.16629e-07 6.07788e-07 1.23206e-06 1.82566e-06 1.99712e-06 1.64661e-06 1.15727e-06 1.13037e-06 2.00334e-06 3.74653e-06 5.65838e-06 6.69638e-06 6.51329e-06 6.11838e-06 7.12801e-06 1.03834e-05 1.54732e-05 2.1482e-05 2.81466e-05 3.62418e-05 4.65938e-05 5.88312e-05 7.1988e-05 8.65665e-05 0.000104683 0.000127599 0.000154856 0.000186585 0.000224489 0.000269051 0.000317408 0.000365467 0.00041113 0.000454754 0.000498135 0.000544204 0.000595869 0.000653319 0.000712994 0.00077012 0.000821642 0.000866688 0.000906372 0.000943346 0.000978844 0.00101033 0.0010347 0.00105224 0.001065 0.0010721 0.00106876 0.00104981 0.00101454 0.000968553 0.000920849 0.000877529 0.00083747 0.000793942 0.000740792 0.000677698 0.000609855 0.000543477 0.000482593 0.000429068 0.000382819 0.000341445 0.000301687 0.000262388 0.000225148 0.000191879 0.000162911 0.000137542 0.000115315 9.61992e-05 8.02374e-05 6.74284e-05 5.75072e-05 4.96642e-05 4.27767e-05 3.6092e-05 2.95681e-05 2.35746e-05 1.83968e-05 1.4038e-05 1.04126e-05 7.55817e-06 5.54646e-06 4.26723e-06 3.43576e-06 2.80637e-06 2.26878e-06 1.76213e-06 1.24318e-06 7.47724e-07 3.66004e-07 1.41944e-07 4.27615e-08 1.012e-08 simulation:estimated_pdf_x: 4373.86 4406.91 4439.95 4473 4506.05 4539.1 4572.14 4605.19 4638.24 4671.29 4704.33 4737.38 4770.43 4803.48 4836.52 4869.57 4902.62 4935.67 4968.72 5001.76 5034.81 5067.86 5100.91 5133.95 5167 5200.05 5233.1 5266.14 5299.19 5332.24 5365.29 5398.33 5431.38 5464.43 5497.48 5530.52 5563.57 5596.62 5629.67 5662.71 5695.76 5728.81 5761.86 5794.9 5827.95 5861 5894.05 5927.09 5960.14 5993.19 6026.24 6059.28 6092.33 6125.38 6158.43 6191.47 6224.52 6257.57 6290.62 6323.66 6356.71 6389.76 6422.81 6455.85 6488.9 6521.95 6555 6588.04 6621.09 6654.14 6687.19 6720.23 6753.28 6786.33 6819.38 6852.42 6885.47 6918.52 6951.57 6984.62 7017.66 7050.71 7083.76 7116.81 7149.85 7182.9 7215.95 7249 7282.04 7315.09 7348.14 7381.19 7414.23 7447.28 7480.33 7513.38 7546.42 7579.47 7612.52 7645.57 simulation:max: 7451.21 simulation:mean: 6031.19 simulation:min: 4536.83 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 5175.07 5426.52 5550.45 5644.41 5718.45 5779.97 5834.1 5889.67 5937.82 5985.49 6032.4 6078.15 6124.28 6171.1 6223.61 6281.6 6340.02 6409.78 6503.6 6643.99 6929.86 simulation:std: 372.414 simulation:var_n_PR: 228.354 simulation:var_n_PR_rand: 0.034061 *** PCCF calculations for the following parameters *** protein (and probe) species: 250594 number protein polonies each protein: 1995 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 250594; n_protein_polonies: 1995; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.02486e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 5.0519e-13; P_minus_free: 1.33017e-13; P_tot_free: 6.38207e-13; PR_complex_conc: 3.2256e-14; PX_complex_conc: 1.27641e-13; XR_complex_conc: 1.27641e-13; f_PR_unnorm: 1.8144e-14; f_PX_unnorm: 7.1798e-14; f_P_unnorm: 5.08636e-13; f_XR_unnorm: 7.1798e-14; f_R_unnorm: 3.2927e-13; f_PR: 0.0181504; f_PX: 0.0718235; f_P: 0.508816; f_XR: 0.0718235; f_R: 0.329386; f_P_all: 0.59879; n_PR: 60.4721; n_PX: 239.296; n_P: 1695.23; n_XR: 239.296; n_R: 1097.42; n_R_all: 1397.19; rho_R: 712916; n_PR_rand: 0.00849219; n_PR_tot: 60.4806; lambda_R: 745168; n_PR_completely_random: 0.00915384; PCCF: 6607.14; *** PCCF simulation results *** PCCF: 6607.14 simulation:coefficient_of_variation: 0.121806 simulation:estimated_pdf: 5.32784e-09 4.46649e-08 1.8481e-07 3.91123e-07 4.41021e-07 3.00637e-07 2.35527e-07 3.48037e-07 4.41926e-07 4.23363e-07 5.59095e-07 1.14654e-06 2.13472e-06 3.1449e-06 3.84562e-06 4.34268e-06 5.01107e-06 6.09536e-06 7.83262e-06 1.0581e-05 1.46618e-05 2.03508e-05 2.75217e-05 3.53194e-05 4.29948e-05 5.08112e-05 5.9641e-05 7.01774e-05 8.29069e-05 9.85223e-05 0.00011762 0.000139803 0.000163999 0.000189698 0.000216719 0.000244183 0.000270589 0.000294547 0.000315707 0.000335443 0.000356343 0.00038026 0.000406459 0.000431952 0.00045425 0.000473243 0.00048929 0.000500472 0.000503544 0.000497465 0.000484759 0.000469648 0.000455199 0.00044175 0.000427902 0.000412942 0.000397457 0.000381561 0.000363881 0.000343074 0.000319426 0.00029443 0.000269533 0.00024574 0.000223654 0.000203185 0.000183539 0.000163932 0.000144354 0.000125544 0.00010837 9.32421e-05 8.00445e-05 6.85005e-05 5.85061e-05 5.00576e-05 4.29448e-05 3.66969e-05 3.08688e-05 2.52952e-05 2.01138e-05 1.56084e-05 1.2027e-05 9.42127e-06 7.60675e-06 6.2823e-06 5.20603e-06 4.26712e-06 3.44332e-06 2.73026e-06 2.10928e-06 1.56419e-06 1.09359e-06 7.07731e-07 4.15124e-07 2.16073e-07 9.76923e-08 3.73921e-08 1.20465e-08 3.13496e-09 simulation:estimated_pdf_x: 3148.72 3218.3 3287.88 3357.46 3427.04 3496.62 3566.2 3635.78 3705.36 3774.94 3844.52 3914.1 3983.68 4053.26 4122.84 4192.42 4262 4331.58 4401.16 4470.74 4540.32 4609.9 4679.48 4749.06 4818.64 4888.22 4957.8 5027.38 5096.97 5166.55 5236.13 5305.71 5375.29 5444.87 5514.45 5584.03 5653.61 5723.19 5792.77 5862.35 5931.93 6001.51 6071.09 6140.67 6210.25 6279.83 6349.41 6418.99 6488.57 6558.15 6627.73 6697.31 6766.89 6836.47 6906.05 6975.63 7045.21 7114.79 7184.37 7253.95 7323.53 7393.11 7462.69 7532.27 7601.85 7671.43 7741.01 7810.59 7880.17 7949.75 8019.33 8088.91 8158.49 8228.07 8297.65 8367.23 8436.81 8506.39 8575.97 8645.55 8715.13 8784.71 8854.29 8923.87 8993.45 9063.03 9132.61 9202.2 9271.78 9341.36 9410.94 9480.52 9550.1 9619.68 9689.26 9758.84 9828.42 9898 9967.58 10037.2 simulation:max: 9583.2 simulation:mean: 6596.92 simulation:min: 3406.27 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 4790.73 5318.07 5582.72 5758.25 5910.81 6050.67 6164.94 6272.24 6380.33 6470.6 6570.43 6668.46 6780.92 6894.86 7010.76 7141.14 7270.17 7439.22 7648.95 7939.09 8514.36 simulation:std: 803.547 simulation:var_n_PR: 58.5427 simulation:var_n_PR_rand: 0.00980689 *** PCCF calculations for the following parameters *** protein (and probe) species: 500000 number protein polonies each protein: 1000 K_d of targeting probes (M): 1e-10 K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500000; n_protein_polonies: 1000; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: 1e-10; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.72414e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.5974e-13; P_minus_free: 6.66667e-14; P_tot_free: 3.26407e-13; PR_complex_conc: 8.31182e-15; PX_complex_conc: 6.52813e-14; XR_complex_conc: 6.52813e-14; f_PR_unnorm: 4.6754e-15; f_PX_unnorm: 3.67207e-14; f_P_unnorm: 2.58604e-13; f_XR_unnorm: 3.67207e-14; f_R_unnorm: 1.63799e-13; f_PR: 0.00934109; f_PX: 0.0733652; f_P: 0.516671; f_XR: 0.0733652; f_R: 0.327257; f_P_all: 0.599377; n_PR: 15.5847; n_PX: 122.402; n_P: 862.013; n_XR: 122.402; n_R: 545.996; n_R_all: 683.983; rho_R: 356479; n_PR_rand: 0.00216082; n_PR_tot: 15.5868; lambda_R: 364791; n_PR_completely_random: 0.00224621; PCCF: 6939.17; *** PCCF simulation results *** PCCF: 6939.17 simulation:coefficient_of_variation: 0.249167 simulation:estimated_pdf: 6.29036e-09 3.59074e-07 1.1003e-06 4.92921e-07 1.5046e-07 1.06293e-06 2.78384e-06 4.65646e-06 7.67236e-06 9.20567e-06 1.16874e-05 1.73953e-05 2.18135e-05 2.48331e-05 3.01124e-05 3.84677e-05 4.93481e-05 6.06399e-05 6.92663e-05 7.70419e-05 8.77945e-05 0.000100709 0.000112989 0.000124408 0.000136276 0.00014898 0.000161947 0.000174277 0.000185296 0.000195123 0.000204185 0.00021239 0.000219213 0.000224081 0.000226568 0.000226597 0.000224522 0.000220957 0.000216589 0.000211991 0.000207382 0.000202453 0.000196583 0.000189281 0.000180408 0.000170027 0.000158267 0.000145469 0.000132281 0.000119452 0.000107552 9.68886e-05 8.7564e-05 7.94917e-05 7.23447e-05 6.56132e-05 5.8824e-05 5.17951e-05 4.47375e-05 3.81087e-05 3.23315e-05 2.7572e-05 2.36986e-05 2.04238e-05 1.74975e-05 1.483e-05 1.24724e-05 1.04997e-05 8.91443e-06 7.63122e-06 6.53579e-06 5.54799e-06 4.63221e-06 3.7793e-06 2.98761e-06 2.26508e-06 1.63344e-06 1.12201e-06 7.48092e-07 5.04987e-07 3.65224e-07 2.94445e-07 2.63975e-07 2.54181e-07 2.51538e-07 2.46004e-07 2.31955e-07 2.09818e-07 1.84387e-07 1.60613e-07 1.39689e-07 1.1928e-07 9.68779e-08 7.2537e-08 4.89939e-08 2.94502e-08 1.56373e-08 7.27734e-09 2.98808e-09 1.07381e-09 simulation:estimated_pdf_x: 1431.93 1582.15 1732.38 1882.61 2032.84 2183.07 2333.29 2483.52 2633.75 2783.98 2934.21 3084.43 3234.66 3384.89 3535.12 3685.34 3835.57 3985.8 4136.03 4286.26 4436.48 4586.71 4736.94 4887.17 5037.4 5187.62 5337.85 5488.08 5638.31 5788.54 5938.76 6088.99 6239.22 6389.45 6539.67 6689.9 6840.13 6990.36 7140.59 7290.81 7441.04 7591.27 7741.5 7891.73 8041.95 8192.18 8342.41 8492.64 8642.87 8793.09 8943.32 9093.55 9243.78 9394.01 9544.23 9694.46 9844.69 9994.92 10145.1 10295.4 10445.6 10595.8 10746.1 10896.3 11046.5 11196.7 11347 11497.2 11647.4 11797.7 11947.9 12098.1 12248.3 12398.6 12548.8 12699 12849.2 12999.5 13149.7 13299.9 13450.2 13600.4 13750.6 13900.8 14051.1 14201.3 14351.5 14501.8 14652 14802.2 14952.4 15102.7 15252.9 15403.1 15553.3 15703.6 15853.8 16004 16154.3 16304.5 simulation:max: 15060.7 simulation:mean: 6940.24 simulation:min: 1675.57 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 3235.33 4169.88 4744.82 5143.23 5461.03 5729.54 5983.26 6210.97 6435.72 6653.1 6869.09 7091.24 7324.79 7574.61 7818.49 8080.87 8360.56 8718.13 9191.78 9894.12 11213.4 simulation:std: 1729.28 simulation:var_n_PR: 15.7063 simulation:var_n_PR_rand: 0.0029913 *** PCCF calculations for the following parameters *** protein (and probe) species: 500 number protein polonies each protein: 1e+06 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500; n_protein_polonies: 1e+06; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 2e-08; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.66667e-10; P_minus_free: 6.66667e-11; P_tot_free: 3.33333e-10; PR_complex_conc: 1.33333e-13; PX_complex_conc: 6.65333e-11; XR_complex_conc: 6.65333e-11; f_PR_unnorm: 7.5e-14; f_PX_unnorm: 3.7425e-11; f_P_unnorm: 2.625e-10; f_XR_unnorm: 3.7425e-11; f_R_unnorm: 1.625e-10; f_PR: 0.000150023; f_PX: 0.0748612; f_P: 0.525079; f_XR: 0.0748612; f_R: 0.325049; f_P_all: 0.60009; n_PR: 250; n_PX: 124750; n_P: 875000; n_XR: 124750; n_R: 541667; n_R_all: 666667; rho_R: 3.55422e+08; n_PR_rand: 2187.97; n_PR_tot: 2437.97; lambda_R: 3.55556e+08; n_PR_completely_random: 2189.34; PCCF: 1.11356; *** PCCF simulation results *** PCCF: 1.11356 simulation:coefficient_of_variation: 0.0201718 simulation:estimated_pdf: 0.000118627 0.000462952 0.00147262 0.00379773 0.00815164 0.0149499 0.0242052 0.0355665 0.0480678 0.059935 0.0695982 0.0781872 0.0906781 0.113644 0.151229 0.203503 0.267874 0.340534 0.417669 0.499998 0.598475 0.729692 0.902377 1.11191 1.35104 1.62208 1.93625 2.303 2.72763 3.21928 3.79037 4.43802 5.13342 5.84174 6.54887 7.26845 8.03483 8.89142 9.85714 10.887 11.8877 12.7878 13.5738 14.2538 14.8252 15.2983 15.7234 16.1584 16.6175 17.0651 17.4443 17.6934 17.7424 17.5305 17.0582 16.4132 15.7238 15.0748 14.4683 13.8483 13.1567 12.3825 11.5665 10.7531 9.94265 9.10558 8.23137 7.34458 6.48457 5.68754 4.97603 4.34625 3.77239 3.23609 2.74456 2.3144 1.9509 1.6475 1.39251 1.16959 0.963079 0.7688 0.594965 0.451962 0.344762 0.272535 0.229837 0.206539 0.189314 0.167569 0.13909 0.108104 0.079203 0.0541012 0.0332531 0.0175907 0.00771574 0.00273398 0.000771334 0.000168501 simulation:estimated_pdf_x: 1.01666 1.01855 1.02045 1.02234 1.02424 1.02613 1.02803 1.02992 1.03182 1.03371 1.03561 1.0375 1.0394 1.04129 1.04319 1.04508 1.04698 1.04887 1.05077 1.05266 1.05456 1.05645 1.05835 1.06024 1.06214 1.06403 1.06593 1.06782 1.06972 1.07161 1.07351 1.0754 1.0773 1.07919 1.08109 1.08298 1.08488 1.08677 1.08867 1.09056 1.09246 1.09436 1.09625 1.09815 1.10004 1.10194 1.10383 1.10573 1.10762 1.10952 1.11141 1.11331 1.1152 1.1171 1.11899 1.12089 1.12278 1.12468 1.12657 1.12847 1.13036 1.13226 1.13415 1.13605 1.13794 1.13984 1.14173 1.14363 1.14552 1.14742 1.14931 1.15121 1.1531 1.155 1.15689 1.15879 1.16068 1.16258 1.16447 1.16637 1.16826 1.17016 1.17205 1.17395 1.17584 1.17774 1.17963 1.18153 1.18342 1.18532 1.18721 1.18911 1.191 1.1929 1.19479 1.19669 1.19858 1.20048 1.20237 1.20427 simulation:max: 1.19321 simulation:mean: 1.11326 simulation:min: 1.02839 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 1.06104 1.07659 1.08451 1.09014 1.09418 1.09801 1.10122 1.10451 1.10762 1.11047 1.11341 1.11613 1.11877 1.12177 1.12509 1.12859 1.13231 1.13674 1.142 1.15031 1.16577 simulation:std: 0.0224565 simulation:var_n_PR: 250.51 simulation:var_n_PR_rand: 2184.09 *** PCCF calculations for the following parameters *** protein (and probe) species: 998 number protein polonies each protein: 501002 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 998; n_protein_polonies: 501002; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.002e-08; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.33601e-10; P_minus_free: 3.34001e-11; P_tot_free: 1.67001e-10; PR_complex_conc: 3.34671e-14; PX_complex_conc: 3.33667e-11; XR_complex_conc: 3.33667e-11; f_PR_unnorm: 1.88252e-14; f_PX_unnorm: 1.87687e-11; f_P_unnorm: 1.31513e-10; f_XR_unnorm: 1.87687e-11; f_R_unnorm: 8.14128e-11; f_PR: 7.51559e-05; f_PX: 0.0749305; f_P: 0.525039; f_XR: 0.0749305; f_R: 0.325024; f_P_all: 0.600045; n_PR: 62.7508; n_PX: 62562.5; n_P: 438377; n_XR: 62562.5; n_R: 271376; n_R_all: 334001; rho_R: 1.78101e+08; n_PR_rand: 549.359; n_PR_tot: 612.11; lambda_R: 1.78134e+08; n_PR_completely_random: 549.531; PCCF: 1.11388; *** PCCF simulation results *** PCCF: 1.11388 simulation:coefficient_of_variation: 0.0405256 simulation:estimated_pdf: 0.000112557 0.000441304 0.00141005 0.00367294 0.00789848 0.014332 0.0226994 0.0326646 0.0442153 0.0570356 0.0701469 0.0830812 0.0975201 0.116758 0.143554 0.179367 0.225154 0.281244 0.346423 0.418837 0.49925 0.592347 0.703438 0.834289 0.982675 1.14557 1.3227 1.51891 1.74442 2.01062 2.32291 2.6754 3.05472 3.45033 3.86125 4.29188 4.74103 5.1939 5.62324 6.00133 6.32001 6.6007 6.88085 7.18778 7.52269 7.86362 8.18246 8.46054 8.69139 8.87213 8.99223 9.02642 8.94186 8.72561 8.40804 8.04877 7.69346 7.34641 6.98588 6.60117 6.2119 5.8498 5.52269 5.20008 4.83643 4.41006 3.9401 3.46833 3.02945 2.63697 2.28824 1.97732 1.70476 1.4743 1.28115 1.10996 0.948225 0.797959 0.670843 0.573946 0.501793 0.440293 0.377575 0.311848 0.250373 0.201265 0.166913 0.143928 0.125939 0.106585 0.083124 0.0584228 0.0377442 0.0234474 0.0142054 0.00796305 0.00382142 0.0014834 0.000450676 0.00010693 simulation:estimated_pdf_x: 0.93075 0.934414 0.938079 0.941744 0.945408 0.949073 0.952738 0.956402 0.960067 0.963732 0.967396 0.971061 0.974726 0.97839 0.982055 0.98572 0.989384 0.993049 0.996714 1.00038 1.00404 1.00771 1.01137 1.01504 1.0187 1.02237 1.02603 1.0297 1.03336 1.03703 1.04069 1.04435 1.04802 1.05168 1.05535 1.05901 1.06268 1.06634 1.07001 1.07367 1.07734 1.081 1.08467 1.08833 1.092 1.09566 1.09932 1.10299 1.10665 1.11032 1.11398 1.11765 1.12131 1.12498 1.12864 1.13231 1.13597 1.13964 1.1433 1.14697 1.15063 1.15429 1.15796 1.16162 1.16529 1.16895 1.17262 1.17628 1.17995 1.18361 1.18728 1.19094 1.19461 1.19827 1.20194 1.2056 1.20926 1.21293 1.21659 1.22026 1.22392 1.22759 1.23125 1.23492 1.23858 1.24225 1.24591 1.24958 1.25324 1.25691 1.26057 1.26423 1.2679 1.27156 1.27523 1.27889 1.28256 1.28622 1.28989 1.29355 simulation:max: 1.2721 simulation:mean: 1.11405 simulation:min: 0.954102 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 1.00715 1.04027 1.05667 1.06751 1.07578 1.08359 1.09061 1.09708 1.10297 1.10877 1.11415 1.11985 1.12493 1.13104 1.1374 1.14418 1.15196 1.16112 1.17122 1.188 1.22154 simulation:std: 0.0451476 simulation:var_n_PR: 63.0379 simulation:var_n_PR_rand: 555.526 *** PCCF calculations for the following parameters *** protein (and probe) species: 1991 number protein polonies each protein: 251130 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 1991; n_protein_polonies: 251130; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 5.0226e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 6.6968e-11; P_minus_free: 1.6742e-11; P_tot_free: 8.371e-11; PR_complex_conc: 8.40884e-15; PX_complex_conc: 1.67336e-11; XR_complex_conc: 1.67336e-11; f_PR_unnorm: 4.72997e-15; f_PX_unnorm: 9.41265e-12; f_P_unnorm: 6.59216e-11; f_XR_unnorm: 9.41265e-12; f_R_unnorm: 4.08086e-11; f_PR: 3.76709e-05; f_PX: 0.0749652; f_P: 0.52502; f_XR: 0.0749652; f_R: 0.325012; f_P_all: 0.600023; n_PR: 15.7666; n_PX: 31375.5; n_P: 219739; n_XR: 31375.5; n_R: 136029; n_R_all: 167420; rho_R: 8.92823e+07; n_PR_rand: 138.052; n_PR_tot: 153.818; lambda_R: 8.92907e+07; n_PR_completely_random: 138.074; PCCF: 1.11403; *** PCCF simulation results *** PCCF: 1.11403 simulation:coefficient_of_variation: 0.0809557 simulation:estimated_pdf: 3.35906e-05 0.000159783 0.000567516 0.00151796 0.00310328 0.0050118 0.00687194 0.00912711 0.0132165 0.020509 0.0312991 0.04531 0.0625974 0.0829379 0.104843 0.127534 0.153999 0.190818 0.245759 0.326412 0.436554 0.569631 0.70863 0.837734 0.956587 1.08262 1.23752 1.42977 1.65261 1.89477 2.1447 2.38637 2.60529 2.80239 2.99295 3.1904 3.39664 3.60461 3.80561 3.99226 4.15386 4.27739 4.36192 4.42083 4.45436 4.43425 4.33411 4.16355 3.95718 3.74213 3.52772 3.31714 3.11473 2.91815 2.71211 2.48261 2.23591 1.99587 1.7821 1.59373 1.41657 1.24221 1.07458 0.920369 0.781474 0.655208 0.538153 0.431987 0.343707 0.276239 0.224106 0.181539 0.148045 0.123522 0.104437 0.0870297 0.069766 0.052645 0.0371367 0.0253167 0.0172673 0.0112803 0.00657888 0.00381138 0.00326669 0.00403695 0.00476743 0.00458886 0.00347939 0.0020609 0.00107058 0.000906796 0.00152375 0.00242288 0.00282369 0.00233117 0.00135594 0.00055406 0.00015864 3.1745e-05 simulation:estimated_pdf_x: 0.751225 0.759561 0.767897 0.776234 0.78457 0.792907 0.801243 0.80958 0.817916 0.826253 0.834589 0.842926 0.851262 0.859599 0.867935 0.876272 0.884608 0.892944 0.901281 0.909617 0.917954 0.92629 0.934627 0.942963 0.9513 0.959636 0.967973 0.976309 0.984646 0.992982 1.00132 1.00965 1.01799 1.02633 1.03466 1.043 1.05134 1.05967 1.06801 1.07635 1.08468 1.09302 1.10136 1.10969 1.11803 1.12637 1.1347 1.14304 1.15137 1.15971 1.16805 1.17638 1.18472 1.19306 1.20139 1.20973 1.21807 1.2264 1.23474 1.24308 1.25141 1.25975 1.26809 1.27642 1.28476 1.29309 1.30143 1.30977 1.3181 1.32644 1.33478 1.34311 1.35145 1.35979 1.36812 1.37646 1.3848 1.39313 1.40147 1.40981 1.41814 1.42648 1.43481 1.44315 1.45149 1.45982 1.46816 1.4765 1.48483 1.49317 1.50151 1.50984 1.51818 1.52652 1.53485 1.54319 1.55153 1.55986 1.5682 1.57653 simulation:max: 1.5344 simulation:mean: 1.11348 simulation:min: 0.796317 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0.912507 0.965807 0.997766 1.01925 1.03677 1.0521 1.066 1.07898 1.09053 1.10188 1.11352 1.12434 1.13484 1.14697 1.15956 1.17376 1.1896 1.20713 1.23024 1.26385 1.32622 simulation:std: 0.0901426 simulation:var_n_PR: 15.2494 simulation:var_n_PR_rand: 140.199 *** PCCF calculations for the following parameters *** protein (and probe) species: 3972 number protein polonies each protein: 125881 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 3972; n_protein_polonies: 125881; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 2.51762e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 3.35683e-11; P_minus_free: 8.39208e-12; P_tot_free: 4.19604e-11; PR_complex_conc: 2.11281e-15; PX_complex_conc: 8.38997e-12; XR_complex_conc: 8.38997e-12; f_PR_unnorm: 1.18846e-15; f_PX_unnorm: 4.71936e-12; f_P_unnorm: 3.30438e-11; f_XR_unnorm: 4.71936e-12; f_R_unnorm: 2.04557e-11; f_PR: 1.88825e-05; f_PX: 0.0749825; f_P: 0.52501; f_XR: 0.0749825; f_R: 0.325006; f_P_all: 0.600011; n_PR: 3.96151; n_PX: 15731.2; n_P: 110146; n_XR: 15731.2; n_R: 68185.5; n_R_all: 83920.7; rho_R: 4.47556e+07; n_PR_rand: 34.6896; n_PR_tot: 38.6511; lambda_R: 4.47577e+07; n_PR_completely_random: 34.6924; PCCF: 1.11411; *** PCCF simulation results *** PCCF: 1.11411 simulation:coefficient_of_variation: 0.161431 simulation:estimated_pdf: 1.67989e-05 8.0889e-05 0.000276614 0.000678496 0.00121617 0.00166079 0.00190715 0.00217768 0.00279765 0.00388846 0.00542979 0.00742231 0.0101 0.0140464 0.0201421 0.0292012 0.0415972 0.0576997 0.0782568 0.104273 0.135672 0.171532 0.210727 0.252722 0.297593 0.346817 0.404063 0.473713 0.557912 0.653298 0.7554 0.864292 0.984213 1.1133 1.24028 1.35338 1.45262 1.54926 1.65144 1.75613 1.8547 1.94387 2.02273 2.08822 2.13556 2.16376 2.17421 2.16669 2.14019 2.09772 2.04888 2.00006 1.94908 1.88893 1.81914 1.74386 1.66498 1.57799 1.47895 1.37053 1.25912 1.14689 1.03084 0.910837 0.792554 0.684117 0.590146 0.511719 0.447266 0.394847 0.351554 0.31283 0.272848 0.228737 0.183807 0.144918 0.116428 0.0971792 0.0831781 0.0714735 0.0614364 0.0529569 0.0452602 0.0375367 0.0299158 0.0231125 0.0177408 0.0139909 0.0116349 0.0103377 0.00986999 0.00987881 0.00962574 0.00840858 0.00620199 0.00372332 0.00177387 0.000658295 0.000187682 4.0454e-05 simulation:estimated_pdf_x: 0.380785 0.396187 0.411589 0.426991 0.442393 0.457795 0.473196 0.488598 0.504 0.519402 0.534804 0.550206 0.565608 0.58101 0.596412 0.611814 0.627216 0.642618 0.65802 0.673422 0.688823 0.704225 0.719627 0.735029 0.750431 0.765833 0.781235 0.796637 0.812039 0.827441 0.842843 0.858245 0.873647 0.889048 0.90445 0.919852 0.935254 0.950656 0.966058 0.98146 0.996862 1.01226 1.02767 1.04307 1.05847 1.07387 1.08927 1.10468 1.12008 1.13548 1.15088 1.16628 1.18169 1.19709 1.21249 1.22789 1.24329 1.25869 1.2741 1.2895 1.3049 1.3203 1.3357 1.35111 1.36651 1.38191 1.39731 1.41271 1.42812 1.44352 1.45892 1.47432 1.48972 1.50513 1.52053 1.53593 1.55133 1.56673 1.58214 1.59754 1.61294 1.62834 1.64374 1.65914 1.67455 1.68995 1.70535 1.72075 1.73615 1.75156 1.76696 1.78236 1.79776 1.81316 1.82857 1.84397 1.85937 1.87477 1.89017 1.90558 simulation:max: 1.81922 simulation:mean: 1.11165 simulation:min: 0.461102 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0.718577 0.831969 0.890522 0.923322 0.954376 0.983066 1.01082 1.03804 1.06457 1.09005 1.1022 1.12676 1.15335 1.18096 1.20793 1.236 1.26566 1.29737 1.33856 1.41211 1.55223 simulation:std: 0.179454 simulation:var_n_PR: 3.8901 simulation:var_n_PR_rand: 34.9881 *** PCCF calculations for the following parameters *** protein (and probe) species: 7924 number protein polonies each protein: 63099 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 7924; n_protein_polonies: 63099; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.26199e-09; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.68265e-11; P_minus_free: 4.20663e-12; P_tot_free: 2.10331e-11; PR_complex_conc: 5.30872e-16; PX_complex_conc: 4.2061e-12; XR_complex_conc: 4.2061e-12; f_PR_unnorm: 2.98615e-16; f_PX_unnorm: 2.36593e-12; f_P_unnorm: 1.65636e-11; f_XR_unnorm: 2.36593e-12; f_R_unnorm: 1.02537e-11; f_PR: 9.46501e-06; f_PX: 0.0749912; f_P: 0.525005; f_XR: 0.0749912; f_R: 0.325003; f_P_all: 0.600006; n_PR: 0.995378; n_PX: 7886.38; n_P: 55211.6; n_XR: 7886.38; n_R: 34178.6; n_R_all: 42066; rho_R: 2.24347e+07; n_PR_rand: 8.71648; n_PR_tot: 9.71186; lambda_R: 2.24352e+07; n_PR_completely_random: 8.71683; PCCF: 1.11415; *** PCCF simulation results *** PCCF: 1.11415 simulation:coefficient_of_variation: 0.322427 simulation:estimated_pdf: 1.30701e-05 0.00010894 0.000446091 0.000947547 0.00116542 0.00133354 0.00251563 0.00455527 0.00598599 0.00847274 0.0165051 0.0280906 0.0347704 0.0389153 0.0545812 0.0802723 0.0972346 0.107658 0.138299 0.187808 0.22088 0.234504 0.27041 0.336125 0.387075 0.414728 0.469384 0.562131 0.63536 0.670902 0.727354 0.825993 0.901231 0.918294 0.934195 0.989898 1.03876 1.0432 1.04457 1.08051 1.11334 1.10413 1.08102 1.08019 1.07855 1.04318 0.992859 0.961016 0.937711 0.895856 0.841889 0.798983 0.7661 0.727831 0.686894 0.654244 0.623415 0.578761 0.521956 0.467433 0.421022 0.37776 0.337157 0.303901 0.277887 0.253359 0.228342 0.205335 0.184855 0.164024 0.142192 0.122004 0.105216 0.0908105 0.077833 0.0667451 0.0577423 0.0498429 0.0422866 0.035285 0.0291537 0.023715 0.0188706 0.0149253 0.012139 0.0103956 0.00940522 0.00885038 0.00828809 0.00728819 0.00580504 0.00420343 0.00286932 0.00190162 0.00119954 0.000680397 0.000328374 0.000129659 4.14165e-05 1.06161e-05 simulation:estimated_pdf_x: -0.105972 -0.077544 -0.049116 -0.020688 0.00773998 0.036168 0.064596 0.093024 0.121452 0.14988 0.178308 0.206736 0.235164 0.263592 0.29202 0.320448 0.348876 0.377304 0.405732 0.43416 0.462588 0.491016 0.519444 0.547872 0.5763 0.604728 0.633156 0.661584 0.690012 0.71844 0.746868 0.775296 0.803724 0.832152 0.86058 0.889008 0.917436 0.945864 0.974292 1.00272 1.03115 1.05958 1.088 1.11643 1.14486 1.17329 1.20172 1.23014 1.25857 1.287 1.31543 1.34386 1.37228 1.40071 1.42914 1.45757 1.486 1.51442 1.54285 1.57128 1.59971 1.62814 1.65656 1.68499 1.71342 1.74185 1.77028 1.7987 1.82713 1.85556 1.88399 1.91242 1.94084 1.96927 1.9977 2.02613 2.05456 2.08298 2.11141 2.13984 2.16827 2.1967 2.22512 2.25355 2.28198 2.31041 2.33884 2.36726 2.39569 2.42412 2.45255 2.48098 2.5094 2.53783 2.56626 2.59469 2.62312 2.65154 2.67997 2.7084 simulation:max: 2.52872 simulation:mean: 1.11708 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0.345026 0.572101 0.686219 0.793866 0.803296 0.905474 0.916768 0.924124 1.02874 1.03557 1.13566 1.14624 1.15467 1.25857 1.26844 1.37198 1.38645 1.49225 1.60256 1.72821 2.05472 simulation:std: 0.360176 simulation:var_n_PR: 0.996736 simulation:var_n_PR_rand: 8.84928 *** PCCF calculations for the following parameters *** protein (and probe) species: 15811 number protein polonies each protein: 31624 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 15811; n_protein_polonies: 31624; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 6.32471e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 8.43295e-12; P_minus_free: 2.10824e-12; P_tot_free: 1.05412e-11; PR_complex_conc: 1.3334e-16; PX_complex_conc: 2.1081e-12; XR_complex_conc: 2.1081e-12; f_PR_unnorm: 7.50037e-17; f_PX_unnorm: 1.18581e-12; f_P_unnorm: 8.30118e-12; f_XR_unnorm: 1.18581e-12; f_R_unnorm: 5.13883e-12; f_PR: 4.74356e-06; f_PX: 0.0749956; f_P: 0.525002; f_XR: 0.0749956; f_R: 0.325002; f_P_all: 0.600003; n_PR: 0.250016; n_PX: 3952.75; n_P: 27671; n_XR: 3952.75; n_R: 17129.7; n_R_all: 21082.7; rho_R: 1.1244e+07; n_PR_rand: 2.18947; n_PR_tot: 2.43948; lambda_R: 1.12441e+07; n_PR_completely_random: 2.18951; PCCF: 1.11417; *** PCCF simulation results *** PCCF: 1.11417 simulation:coefficient_of_variation: 0.644361 simulation:estimated_pdf: 0.00620792 0.0500016 0.192811 0.393069 0.459803 0.330428 0.155497 0.057782 0.0558737 0.158175 0.377155 0.647954 0.817873 0.77898 0.57638 0.353793 0.230813 0.25278 0.402355 0.612345 0.784009 0.833678 0.745959 0.578391 0.419609 0.336514 0.347796 0.426643 0.520183 0.576195 0.566812 0.498527 0.403899 0.321268 0.275516 0.270022 0.28965 0.311601 0.317033 0.298704 0.261896 0.219373 0.184158 0.163431 0.156588 0.157387 0.157869 0.152255 0.139004 0.120527 0.101218 0.085017 0.073989 0.0676394 0.0639583 0.0606351 0.0561189 0.0500865 0.0432469 0.0367335 0.0314591 0.0277729 0.0254076 0.0237022 0.0220269 0.0200139 0.0176439 0.0151559 0.0128662 0.0109963 0.00959918 0.00857277 0.00772543 0.00689984 0.00602503 0.00512305 0.00427252 0.00355493 0.00301193 0.0026335 0.00236927 0.00215024 0.0019245 0.00166943 0.00139106 0.00111265 0.000859489 0.000648167 0.000484637 0.000361634 0.000269125 0.000197764 0.000141331 9.67084e-05 6.25527e-05 3.77314e-05 2.11657e-05 1.10226e-05 5.32953e-06 2.36797e-06 simulation:estimated_pdf_x: -0.208378 -0.154796 -0.101214 -0.0476317 0.00595059 0.0595329 0.113115 0.166697 0.22028 0.273862 0.327444 0.381026 0.434609 0.488191 0.541773 0.595355 0.648938 0.70252 0.756102 0.809685 0.863267 0.916849 0.970431 1.02401 1.0776 1.13118 1.18476 1.23834 1.29192 1.34551 1.39909 1.45267 1.50625 1.55984 1.61342 1.667 1.72058 1.77417 1.82775 1.88133 1.93491 1.98849 2.04208 2.09566 2.14924 2.20282 2.25641 2.30999 2.36357 2.41715 2.47073 2.52432 2.5779 2.63148 2.68506 2.73865 2.79223 2.84581 2.89939 2.95297 3.00656 3.06014 3.11372 3.1673 3.22089 3.27447 3.32805 3.38163 3.43522 3.4888 3.54238 3.59596 3.64954 3.70313 3.75671 3.81029 3.86387 3.91746 3.97104 4.02462 4.0782 4.13178 4.18537 4.23895 4.29253 4.34611 4.3997 4.45328 4.50686 4.56044 4.61403 4.66761 4.72119 4.77477 4.82835 4.88194 4.93552 4.9891 5.04268 5.09627 simulation:max: 4.52259 simulation:mean: 1.11289 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0.45049 0.454744 0.457096 0.45963 0.467689 0.906238 0.910907 0.914668 0.919004 0.927432 1.3589 1.36719 1.37525 1.38533 1.81455 1.83126 2.24832 2.30101 3.19555 simulation:std: 0.717101 simulation:var_n_PR: 0.25372 simulation:var_n_PR_rand: 2.20322 *** PCCF calculations for the following parameters *** protein (and probe) species: 31548 number protein polonies each protein: 15849 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 31548; n_protein_polonies: 15849; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.16977e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 4.22636e-12; P_minus_free: 1.05659e-12; P_tot_free: 5.28296e-12; PR_complex_conc: 3.34915e-17; PX_complex_conc: 1.05656e-12; XR_complex_conc: 1.05656e-12; f_PR_unnorm: 1.8839e-17; f_PX_unnorm: 5.94314e-13; f_P_unnorm: 4.16033e-12; f_XR_unnorm: 5.94314e-13; f_R_unnorm: 2.57544e-12; f_PR: 2.37734e-06; f_PX: 0.0749978; f_P: 0.525001; f_XR: 0.0749978; f_R: 0.325001; f_P_all: 0.600001; n_PR: 0.0627972; n_PX: 1981.06; n_P: 13867.9; n_XR: 1981.06; n_R: 8584.87; n_R_all: 10566; rho_R: 5.63517e+06; n_PR_rand: 0.549937; n_PR_tot: 0.612734; lambda_R: 5.6352e+06; n_PR_completely_random: 0.549942; PCCF: 1.11418; *** PCCF simulation results *** PCCF: 1.11418 simulation:coefficient_of_variation: 1.28831 simulation:estimated_pdf: 0.0194325 0.173321 0.362455 0.454573 0.453978 0.407162 0.348921 0.295978 0.253455 0.221037 0.196572 0.177732 0.162624 0.149898 0.138676 0.128429 0.118862 0.109827 0.101264 0.0931538 0.0854988 0.078306 0.0715804 0.065321 0.0595206 0.0541663 0.0492401 0.0447207 0.0405841 0.0368053 0.0333589 0.0302196 0.027363 0.0247657 0.0223835 0.0202462 0.0183045 0.0165412 0.0149406 0.0134879 0.0121699 0.0109744 0.00989023 0.00890725 0.00801628 0.00720896 0.00647769 0.00581557 0.00521634 0.00467428 0.00418423 0.00374147 0.0033417 0.00298102 0.00265588 0.00236302 0.00209949 0.0018626 0.00164987 0.00145907 0.00128815 0.00113523 0.000998609 0.000871469 0.000756097 0.00066234 0.00057881 0.000504547 0.000438672 0.000380373 0.000328904 0.000283575 0.000243758 0.000208874 0.000178397 0.000151847 0.000128787 0.000108821 9.15895e-05 7.65735e-05 5.99088e-05 4.97837e-05 4.14545e-05 3.43249e-05 2.82534e-05 2.31107e-05 1.87793e-05 1.51526e-05 1.2135e-05 9.63493e-06 6.88544e-06 4.92487e-06 3.87377e-06 3.01455e-06 2.31529e-06 1.75282e-06 1.30612e-06 8.49644e-07 4.96137e-07 3.6037e-07 simulation:estimated_pdf_x: -0.856414 -0.667396 -0.478378 -0.289359 -0.100341 0.0886768 0.277695 0.466713 0.655731 0.844749 1.03377 1.22279 1.4118 1.60082 1.78984 1.97886 2.16788 2.35689 2.54591 2.73493 2.92395 3.11297 3.30199 3.491 3.68002 3.86904 4.05806 4.24708 4.43609 4.62511 4.81413 5.00315 5.19217 5.38119 5.5702 5.75922 5.94824 6.13726 6.32628 6.51529 6.70431 6.89333 7.08235 7.27137 7.46038 7.6494 7.83842 8.02744 8.21646 8.40548 8.59449 8.78351 8.97253 9.16155 9.35057 9.53958 9.7286 9.91762 10.1066 10.2957 10.4847 10.6737 10.8627 11.0517 11.2407 11.4298 11.6188 11.8078 11.9968 12.1858 12.3749 12.5639 12.7529 12.9419 13.1309 13.3199 13.509 13.698 13.887 14.076 14.265 14.4541 14.6431 14.8321 15.0211 15.2101 15.3991 15.5882 15.7772 15.9662 16.1552 16.3442 16.5333 16.7223 16.9113 17.1003 17.2893 17.4783 17.6674 17.8564 simulation:max: 10.7595 simulation:mean: 1.11232 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 1.77102 1.79688 1.80744 1.81664 1.82577 1.83575 1.85178 3.60615 3.6697 5.49674 simulation:std: 1.43301 simulation:var_n_PR: 0.0638232 simulation:var_n_PR_rand: 0.558971 *** PCCF calculations for the following parameters *** protein (and probe) species: 62946 number protein polonies each protein: 7943 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 62946; n_protein_polonies: 7943; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 1.58866e-10; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.11822e-12; P_minus_free: 5.29554e-13; P_tot_free: 2.64777e-12; PR_complex_conc: 8.41284e-18; PX_complex_conc: 5.29546e-13; XR_complex_conc: 5.29546e-13; f_PR_unnorm: 4.73222e-18; f_PX_unnorm: 2.9787e-13; f_P_unnorm: 2.08512e-12; f_XR_unnorm: 2.9787e-13; f_R_unnorm: 1.29079e-12; f_PR: 1.1915e-06; f_PX: 0.0749989; f_P: 0.525001; f_XR: 0.0749989; f_R: 0.325; f_P_all: 0.600001; n_PR: 0.0157734; n_PX: 992.859; n_P: 6950.12; n_XR: 992.859; n_R: 4302.46; n_R_all: 5295.33; rho_R: 2.82417e+06; n_PR_rand: 0.138128; n_PR_tot: 0.153901; lambda_R: 2.82418e+06; n_PR_completely_random: 0.138128; PCCF: 1.11419; *** PCCF simulation results *** PCCF: 1.11419 simulation:coefficient_of_variation: 2.5341 simulation:estimated_pdf: 0.0355325 0.545997 0.564347 0.389163 0.241133 0.146039 0.0896234 0.0570779 0.0385215 0.0280243 0.0221384 0.0188727 0.0170758 0.0160803 0.0155 0.0151128 0.0147941 0.0144766 0.0141271 0.0137322 0.01329 0.0128047 0.0122837 0.0117355 0.0111687 0.0105915 0.0100112 0.00941601 0.00885253 0.00830048 0.0077638 0.0072455 0.00674779 0.00627223 0.00581983 0.00539113 0.00498629 0.00460518 0.00424742 0.00391243 0.00359949 0.00330777 0.00303636 0.00278431 0.00255062 0.00233429 0.00213432 0.00194972 0.00177951 0.00162277 0.00147858 0.00134608 0.00122443 0.00111286 0.00101061 0.00091698 0.000831316 0.000752998 0.000681447 0.000616124 0.000556527 0.000502191 0.000452683 0.000407604 0.000366585 0.000329284 0.000295389 0.000264609 0.00023668 0.000211358 0.000188418 0.000167655 0.000148882 0.000131925 0.000116628 0.000102846 9.0447e-05 7.93094e-05 6.93228e-05 6.03856e-05 5.24049e-05 4.52954e-05 3.89789e-05 3.27584e-05 2.59108e-05 2.20193e-05 1.87338e-05 1.58173e-05 1.3245e-05 1.09919e-05 9.0331e-06 7.34413e-06 5.90077e-06 4.67942e-06 3.65715e-06 2.81189e-06 2.1225e-06 1.56888e-06 1.05159e-06 5.24823e-07 simulation:estimated_pdf_x: -0.929062 -0.532696 -0.136331 0.260034 0.656399 1.05276 1.44913 1.8455 2.24186 2.63823 3.03459 3.43096 3.82732 4.22369 4.62005 5.01642 5.41278 5.80915 6.20551 6.60188 6.99824 7.39461 7.79097 8.18734 8.58371 8.98007 9.37644 9.7728 10.1692 10.5655 10.9619 11.3583 11.7546 12.151 12.5474 12.9437 13.3401 13.7365 14.1328 14.5292 14.9256 15.3219 15.7183 16.1146 16.511 16.9074 17.3037 17.7001 18.0965 18.4928 18.8892 19.2856 19.6819 20.0783 20.4747 20.871 21.2674 21.6638 22.0601 22.4565 22.8529 23.2492 23.6456 24.042 24.4383 24.8347 25.231 25.6274 26.0238 26.4201 26.8165 27.2129 27.6092 28.0056 28.402 28.7983 29.1947 29.5911 29.9874 30.3838 30.7802 31.1765 31.5729 31.9693 32.3656 32.762 33.1584 33.5547 33.9511 34.3475 34.7438 35.1402 35.5365 35.9329 36.3293 36.7256 37.122 37.5184 37.9147 38.3111 simulation:max: 22.0117 simulation:mean: 1.10889 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7.17403 7.30208 7.5812 simulation:std: 2.81004 simulation:var_n_PR: 0.0159456 simulation:var_n_PR_rand: 0.135662 *** PCCF calculations for the following parameters *** protein (and probe) species: 125594 number protein polonies each protein: 3981 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 125594; n_protein_polonies: 3981; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 7.96216e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 1.06162e-12; P_minus_free: 2.65405e-13; P_tot_free: 1.32703e-12; PR_complex_conc: 2.1132e-18; PX_complex_conc: 2.65403e-13; XR_complex_conc: 2.65403e-13; f_PR_unnorm: 1.18868e-18; f_PX_unnorm: 1.49289e-13; f_P_unnorm: 1.04503e-12; f_XR_unnorm: 1.49289e-13; f_R_unnorm: 6.46926e-13; f_PR: 5.97163e-07; f_PX: 0.0749994; f_P: 0.525; f_XR: 0.0749994; f_R: 0.325; f_P_all: 0.6; n_PR: 0.00396217; n_PX: 497.621; n_P: 3483.37; n_XR: 497.621; n_R: 2156.37; n_R_all: 2654; rho_R: 1.41546e+06; n_PR_rand: 0.0346974; n_PR_tot: 0.0386596; lambda_R: 1.41547e+06; n_PR_completely_random: 0.0346975; PCCF: 1.11419; *** PCCF simulation results *** PCCF: 1.11419 simulation:coefficient_of_variation: 5.01133 simulation:estimated_pdf: 0.0521036 0.397223 0.132194 0.0487186 0.0203439 0.00945412 0.00485575 0.00277557 0.00179134 0.00131564 0.00108717 0.000982472 0.000940329 0.000929212 0.00093245 0.000941096 0.000950354 0.000957716 0.000961958 0.000962587 0.000952667 0.000948063 0.000939557 0.000927674 0.000912895 0.000895652 0.000876338 0.000855302 0.000832858 0.000809287 0.000784836 0.000759725 0.000734147 0.000708275 0.000682258 0.000656227 0.000630297 0.000604567 0.000579123 0.000554039 0.000529379 0.000505195 0.000481534 0.000458432 0.000435921 0.000414026 0.000392765 0.000372155 0.000352205 0.000332924 0.000314314 0.000296378 0.000279113 0.000262516 0.000246582 0.000231303 0.000216671 0.000202676 0.000189308 0.000176553 0.000164399 0.000152834 0.000141843 0.000131412 0.000121526 0.000112171 0.000103329 9.49872e-05 8.71286e-05 7.97378e-05 7.27989e-05 6.62961e-05 6.02137e-05 5.45357e-05 4.92465e-05 4.43303e-05 3.97718e-05 3.55553e-05 3.16657e-05 2.80877e-05 2.48062e-05 2.18066e-05 1.9074e-05 1.65939e-05 1.43522e-05 1.23347e-05 1.05276e-05 8.91724e-06 7.49032e-06 6.23376e-06 5.13477e-06 4.1809e-06 3.36001e-06 2.66034e-06 2.07049e-06 1.57188e-06 8.38504e-07 5.38254e-07 4.00816e-07 2.85214e-07 simulation:estimated_pdf_x: -0.996514 0.122211 1.24094 2.35966 3.47839 4.59711 5.71584 6.83456 7.95329 9.07201 10.1907 11.3095 12.4282 13.5469 14.6656 15.7844 16.9031 18.0218 19.1405 20.2593 21.378 22.4967 23.6154 24.7342 25.8529 26.9716 28.0903 29.2091 30.3278 31.4465 32.5652 33.684 34.8027 35.9214 37.0401 38.1589 39.2776 40.3963 41.515 42.6338 43.7525 44.8712 45.9899 47.1087 48.2274 49.3461 50.4648 51.5836 52.7023 53.821 54.9397 56.0585 57.1772 58.2959 59.4146 60.5334 61.6521 62.7708 63.8895 65.0083 66.127 67.2457 68.3644 69.4832 70.6019 71.7206 72.8393 73.9581 75.0768 76.1955 77.3142 78.433 79.5517 80.6704 81.7891 82.9079 84.0266 85.1453 86.264 87.3828 88.5015 89.6202 90.7389 91.8577 92.9764 94.0951 95.2138 96.3326 97.4513 98.57 99.6887 100.807 101.926 103.045 104.164 105.282 106.401 107.52 108.639 109.757 simulation:max: 59.9571 simulation:mean: 1.13783 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 29.365 simulation:std: 5.70204 simulation:var_n_PR: 0.00328944 simulation:var_n_PR_rand: 0.0356931 *** PCCF calculations for the following parameters *** protein (and probe) species: 250594 number protein polonies each protein: 1995 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 250594; n_protein_polonies: 1995; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 3.99052e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 5.32069e-13; P_minus_free: 1.33017e-13; P_tot_free: 6.65086e-13; PR_complex_conc: 5.30808e-19; PX_complex_conc: 1.33017e-13; XR_complex_conc: 1.33017e-13; f_PR_unnorm: 2.98579e-19; f_PX_unnorm: 7.48219e-14; f_P_unnorm: 5.23756e-13; f_XR_unnorm: 7.48219e-14; f_R_unnorm: 3.2423e-13; f_PR: 2.99289e-07; f_PX: 0.0749997; f_P: 0.525; f_XR: 0.0749997; f_R: 0.325; f_P_all: 0.6; n_PR: 0.000995136; n_PX: 249.374; n_P: 1745.63; n_XR: 249.374; n_R: 1080.62; n_R_all: 1330; rho_R: 709333; n_PR_rand: 0.00871362; n_PR_tot: 0.00970876; lambda_R: 709333; n_PR_completely_random: 0.00871363; PCCF: 1.1142; *** PCCF simulation results *** PCCF: 1.1142 simulation:coefficient_of_variation: 9.95697 simulation:estimated_pdf: 0.0674174 0.118575 0.0233488 0.00682024 0.00250038 0.00106401 0.000504775 0.000261623 0.000147189 9.0271e-05 6.09994e-05 4.5818e-05 3.81667e-05 3.46949e-05 3.36116e-05 3.392e-05 3.16542e-05 3.43105e-05 3.68878e-05 3.93779e-05 4.17746e-05 4.40735e-05 4.62713e-05 4.83659e-05 5.03559e-05 5.22409e-05 5.40208e-05 5.56961e-05 5.72677e-05 5.87367e-05 6.01044e-05 6.13724e-05 6.25424e-05 6.3616e-05 6.45952e-05 6.54817e-05 6.62773e-05 6.69841e-05 6.76037e-05 6.81381e-05 6.85889e-05 6.89581e-05 6.92472e-05 6.9458e-05 6.9592e-05 6.96508e-05 6.9636e-05 6.95489e-05 6.9391e-05 6.91636e-05 6.88681e-05 6.85056e-05 6.80774e-05 6.75846e-05 6.70284e-05 6.64098e-05 6.57299e-05 6.49897e-05 6.41901e-05 6.33323e-05 6.2417e-05 6.14453e-05 6.04181e-05 5.93362e-05 5.82007e-05 5.70125e-05 5.57726e-05 5.44819e-05 5.31415e-05 5.17526e-05 5.03163e-05 4.88337e-05 4.73064e-05 4.57356e-05 4.41231e-05 4.24705e-05 4.07798e-05 3.9053e-05 3.72925e-05 3.5501e-05 3.36813e-05 3.18368e-05 2.9971e-05 2.80882e-05 2.61929e-05 2.42904e-05 2.23866e-05 2.04881e-05 1.86024e-05 1.67377e-05 1.49036e-05 1.31107e-05 1.13706e-05 9.69665e-06 8.10326e-06 6.60633e-06 5.22305e-06 3.97154e-06 2.87031e-06 1.93716e-06 simulation:estimated_pdf_x: -1.02875 1.37236 3.77347 6.17458 8.57568 10.9768 13.3779 15.779 18.1801 20.5812 22.9823 25.3834 27.7846 30.1857 32.5868 34.9879 37.389 39.7901 42.1912 44.5923 46.9934 49.3945 51.7956 54.1967 56.5978 58.999 61.4001 63.8012 66.2023 68.6034 71.0045 73.4056 75.8067 78.2078 80.6089 83.01 85.4111 87.8123 90.2134 92.6145 95.0156 97.4167 99.8178 102.219 104.62 107.021 109.422 111.823 114.224 116.626 119.027 121.428 123.829 126.23 128.631 131.032 133.433 135.834 138.236 140.637 143.038 145.439 147.84 150.241 152.642 155.043 157.444 159.846 162.247 164.648 167.049 169.45 171.851 174.252 176.653 179.054 181.455 183.857 186.258 188.659 191.06 193.461 195.862 198.263 200.664 203.065 205.467 207.868 210.269 212.67 215.071 217.472 219.873 222.274 224.675 227.077 229.478 231.879 234.28 236.681 simulation:max: 125.944 simulation:mean: 1.1506 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 52.3543 simulation:std: 11.4565 simulation:var_n_PR: 0.00089928 simulation:var_n_PR_rand: 0.00901809 *** PCCF calculations for the following parameters *** protein (and probe) species: 500000 number protein polonies each protein: 1000 K_d of targeting probes (M): Inf (non-specific binding only) K_d of non-specific binding (M): 1e-05 number random simulations: 10000 *** PCCF calculation trace *** PCCF P_total: 2e-11; R_total: 2e-10; reaction_volume: 0.0001; n_proteins: 500000; n_protein_polonies: 1000; R0_total_conc: 1e-07; fraction_alpha: 0.8; fraction_tau: 0.8; fraction_beta: 0.75; fraction_gamma: 0.75; U: 1e-05; K_D: Inf; polony_radius: 7e-07; slide_area: 0.001875; traceopt: 1; poisson_simulations: 10000; simulation_prctiles: [1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 99]; K_D_tilde: 2e-11; P_tot_conc: 2e-07; R_tot_conc: 2e-06; P_plus_free: 2.66667e-13; P_minus_free: 6.66667e-14; P_tot_free: 3.33333e-13; PR_complex_conc: 1.33333e-19; PX_complex_conc: 6.66665e-14; XR_complex_conc: 6.66665e-14; f_PR_unnorm: 7.5e-20; f_PX_unnorm: 3.74999e-14; f_P_unnorm: 2.625e-13; f_XR_unnorm: 3.74999e-14; f_R_unnorm: 1.625e-13; f_PR: 1.5e-07; f_PX: 0.0749999; f_P: 0.525; f_XR: 0.0749999; f_R: 0.325; f_P_all: 0.6; n_PR: 0.00025; n_PX: 125; n_P: 875; n_XR: 125; n_R: 541.667; n_R_all: 666.667; rho_R: 355555; n_PR_rand: 0.00218934; n_PR_tot: 0.00243934; lambda_R: 355556; n_PR_completely_random: 0.00218934; PCCF: 1.11419; *** PCCF simulation results *** PCCF: 1.11419 simulation:coefficient_of_variation: 19.2436 simulation:estimated_pdf: 0.107799 0.00589289 0.000762934 0.000192464 6.69176e-05 2.84921e-05 1.41827e-05 8.13303e-06 5.35436e-06 4.01417e-06 2.04807e-06 2.22034e-06 2.37854e-06 2.52367e-06 2.65672e-06 2.77863e-06 2.8903e-06 2.99255e-06 3.08616e-06 3.17181e-06 3.25014e-06 3.32173e-06 3.3871e-06 3.44673e-06 3.50104e-06 3.55042e-06 3.59523e-06 3.6358e-06 3.67241e-06 3.70532e-06 3.73478e-06 3.76101e-06 3.7842e-06 3.80454e-06 3.82218e-06 3.83729e-06 3.84998e-06 3.86039e-06 3.86862e-06 3.87478e-06 3.87896e-06 3.88123e-06 3.88166e-06 3.88033e-06 3.87729e-06 3.87259e-06 3.86627e-06 3.85837e-06 3.84893e-06 3.83796e-06 3.82548e-06 3.81152e-06 3.79607e-06 3.77916e-06 3.76076e-06 3.74089e-06 3.71952e-06 3.69664e-06 3.67224e-06 3.64629e-06 3.61875e-06 3.5896e-06 3.55878e-06 3.52626e-06 3.49198e-06 3.45589e-06 3.41791e-06 3.37798e-06 3.33602e-06 3.29194e-06 3.24564e-06 3.19704e-06 3.14601e-06 3.09244e-06 3.03619e-06 2.97712e-06 2.91509e-06 2.84992e-06 2.78145e-06 2.70948e-06 2.6338e-06 2.5542e-06 2.47045e-06 2.38229e-06 2.28947e-06 2.19172e-06 2.08875e-06 1.98027e-06 1.86601e-06 1.74568e-06 1.61904e-06 1.48592e-06 1.34623e-06 1.20003e-06 1.04765e-06 8.89834e-07 7.27956e-07 5.64452e-07 4.0346e-07 2.51937e-07 simulation:estimated_pdf_x: -1.06406 8.53874 18.1415 27.7443 37.3471 46.9499 56.5527 66.1555 75.7583 85.3611 94.9639 104.567 114.17 123.772 133.375 142.978 152.581 162.184 171.786 181.389 190.992 200.595 210.198 219.8 229.403 239.006 248.609 258.212 267.814 277.417 287.02 296.623 306.226 315.828 325.431 335.034 344.637 354.24 363.842 373.445 383.048 392.651 402.254 411.856 421.459 431.062 440.665 450.268 459.87 469.473 479.076 488.679 498.282 507.884 517.487 527.09 536.693 546.296 555.898 565.501 575.104 584.707 594.31 603.912 613.515 623.118 632.721 642.324 651.926 661.529 671.132 680.735 690.338 699.94 709.543 719.146 728.749 738.352 747.954 757.557 767.16 776.763 786.366 795.968 805.571 815.174 824.777 834.38 843.982 853.585 863.188 872.791 882.394 891.996 901.599 911.202 920.805 930.408 940.01 949.613 simulation:max: 490.288 simulation:mean: 1.23599 simulation:min: 0 simulation:prctile_pcts: 1 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 99 simulation:prctile_values: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 simulation:std: 23.7849 simulation:var_n_PR: 0 simulation:var_n_PR_rand: 0.00269298