Plasmids                                        

 

p415-TOR-HIS3                                           map    seq

 

p415-TOR-link-HIS3HA                              map    seq

 

pUG-spHIS5-FPR1HA                                map    seq

 

pRS306-Rpn10-FPR1                                 map    seq

 

PROTet.e133-TOR-link-HIS3HA               map    seq

 

 

All maps are derived from pDRAW32

 

 

Maps and sequences are not provided for TORS1972R mutant reporters. Please simply refer to the TOR maps. Plasmid pUG-spHIS5-FPR1HA was used to create strain Pre10-FPR1 by double homologous recombination of a linear amplified PCR product. Plasmid pRS306-Rpn10-FPR1 was cut with KasI and used to create strain Rpn10-FPR1 by two-step integration.

 

 

Primers

 

All necessary primers required to construct the above plasmids are included here. Construction of the TORS1972R vectors require the same primers as TOR vectors.