Tm calculator using the Nearest-Neighbor method (NN paramters are based on SantaLucia J Jr. (1998) PNAS, 95:1460-5)

Tm is adjusted for MgCl2 and DMSO concentration based on von Ahsen et al. Clinical Chemistry 47: 1956-61 (2001)

Oligonucleotide Sequence:

Primer concentration (nM):

Sodium/Potasium concentration (mM):

Magnesium concentration (mM):

dNTP concentration (mM):

Percentage of DMSO (%)

Percentage of annealed temlate(%)