Generate PCR primers from sequence. Separate sequences with line returns. Complementarity will follow the IUPAC convention.


          

IUPAC Degeneracies

BaseNameBases RepresentedComplementary Base
AAdenineAT
TThymidineTA
UUridine(RNA only)U A
GGuanidineGC
CCytidineCG
YpYrimidineC TR
RpuRineA GY
SStrong(3Hbonds)G CW
WWeak(2Hbonds)A TS
KKetoT/U GM
MaMinoA CK
Bnot AC G TV
Dnot CA G TH
Hnot GA C TD
Vnot T/UA C GB
NUnknownA C G TN
Source: http://hornet.bio.uci.edu/~hjm/projects/tacg/