Supplemental Figure 1. Dendrogram of metazoan miRNAs based on sequence similarity scores. A set of 305 published miRNA sequences, including redundant miRNAs published in multiple articles (Lagos-Quintana, 2001; Lau, 2001; Lee, 2001; Lagos-Quintana, 2002; Mourelatos, 2002; Lagos-Quintana, 2003; Dostie, 2003), were aligned against each other by a Smith-Waterman algorithm (EMBOSS v2.3.1; Rice, 2000). From the scores of the pair-wise comparisons, a dissimilarity matrix was constructed and used in performing complete hierarchical clustering (see Methods). The dendrogram was generated from the resulting clustering. The green boxes designate the clusters represented in Figure 4 of the main text. For additional sequence representations of clusters, see Supplemental Table II.

Dendrogram Abbreviations and References:

H.s. = Homo sapiens
M.m. = Mus musculus
D.m. = Drosophila melanogaster
C.e. = Caenorhabditis elegans

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