AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i136_Transmembrane_Transport_10_mpneu_reg_300.orf -o136_mpneu_300.ace -a/home/amcguire/alignace/lib/ORF_mpneu.txt -z/skink1/amcguire/genomes/mpneu.fna -g0.40 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.4 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RMP00137 300 Mycoplasma_pneumoniae Motif number 1 gaaattaccacctctacttcccagagtttggg 1 54 1 cctctatccc 0.996846 -247 aaacgtgagtcctccaactcccaaactctggg 1 73 0 cctccatccc 0.998932 -228 gtggttattacccctacatcgagttgactatc 1 114 0 cccctatcga 0.9533 -187 gtaataaccaccttcatttacctgaacttggt 1 135 1 ccttcatacc 0.990724 -166 gactattcccactccagctaccaagttcaggt 1 154 0 actccatacc 0.979961 -147 ctaaaacggaccttcatatcgagtcaaccaat 1 196 0 ccttcatcga 0.982391 -105 ****** **** Masking position 6 Map Score: 7.24678 Number of sites scoring better than the average of aligned sites = 38 Number in coding regions = 35 Number in noncoding regions = 3 Number of orfs with sites within 600 bp upstream = 3 Fraction of orfs with sites within 600 bp upstream = 0.00048185 Motif number 2 gagttgactatctcgtggactaaacgtgag 1 96 0 tctcgtggac 0.994149 -205 tacccctacatcgagttgactatctcgtgg 1 108 0 tcgagttgac 0.993789 -193 gaccttcatatcgagtcaaccaatctcgtg 1 190 0 tcgagtcaac 0.983523 -111 tattaatacttcgcttggacatgcttctaa 1 224 0 tcgcttggac 0.994149 -77 ********** Masking position 6 Map Score: 3.7103 Number of sites scoring better than the average of aligned sites = 9 Number in coding regions = 7 Number in noncoding regions = 2 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 3 atattctaattcaatccgcaaa 1 1 0 tcaatccaaa 0.993064 -300 aatatactgatcaatctgcaaacctagaaatt 1 28 1 tcaatccaaa 0.993064 -273 acgtgagtcctccaactcccaaactctgggaa 1 71 0 tccaacccaa 0.982395 -230 ctattcccactccagctaccaagttcaggtaa 1 152 0 tccagcccaa 0.988053 -149 taaatcggtttcgatcaaaaattcgccagaat 1 258 1 tcgatcaaat 0.894207 -43 aatattaatttcgttcatcaaaaa 1 287 1 tcgttccaaa 0.982024 -14 ****** **** Masking position 11 Map Score: 4.67857 Number of sites scoring better than the average of aligned sites = 59 Number in coding regions = 54 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 2 Fraction of orfs with sites within 600 bp upstream = 0.000321234 Motif number 4 gggaagtagaggtggtaatttctaggtttg 1 46 0 ggtggtaatt 0.976259 -255 aactcgatgtaggggtaataaccaccttca 1 121 1 aggggtaata 0.990468 -180 tggtagctggagtgggaatagtccccacac 1 163 1 agtgggaata 0.990467 -138 gtccaagcgaagtattaatataattaaatc 1 234 1 agtattaata 0.91818 -67 ********** Masking position 7 Map Score: 1.0189 Number of sites scoring better than the average of aligned sites = 71 Number in coding regions = 66 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 5 atattctaattcaatccgcaaa 1 3 0 tcaatccgca 0.996794 -298 aatatactgatcaatctgcaaacctagaaa 1 28 1 tcaatctgca 0.995083 -273 ********** Masking position 5 Map Score: 0.50812 Number of sites scoring better than the average of aligned sites = 4 Number in coding regions = 4 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 7 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 8 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0