AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i193_dna_polymerase_mpneu_reg_300.orf -o193_mpneu_300.ace -a/home/amcguire/alignace/lib/ORF_mpneu.txt -z/skink1/amcguire/genomes/mpneu.fna -g0.40 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.4 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RMP00460 300 Mycoplasma_pneumoniae Motif number 1 tctttgatcttctcaacttttttcttaacc 1 195 1 tctcaacttt 0.996639 -106 tcaacttttttcttaaccttttgtggaaac 1 207 1 tcttaacctt 0.996639 -94 cggtgcatggtcttaactttaatttgcact 1 238 1 tcttaacttt 0.99481 -63 cactttagtttctcaaccttgcatttgatt 1 264 1 tctcaacctt 0.997824 -37 ********** Masking position 5 Map Score: 9.76688 Number of sites scoring better than the average of aligned sites = 1 Number in coding regions = 1 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 2 ctaaacgcgtcaagagtctctaaattatctt 1 8 0 caagagttaa 0.935367 -293 agactcttgacgcgtttagaaataattttaatcg 1 23 1 cgcgtttaaa 0.954303 -278 gttgtgtcagcgagattatgtacagtttcctatt 1 63 1 cgagatttaa 0.98981 -238 ccaagttcgacaagttggaataaaaaaaatagga 1 90 0 caagttgtaa 0.986441 -211 tcgaatcaggcgagtttttccagatgaccaagtt 1 117 0 cgagtttcaa 0.994627 -184 tacagtagatcaagttgtagtaaattttgaaccc 1 159 0 caagttgtaa 0.986441 -142 gaccatgcaccgagtttccacaaaaggttaagaa 1 216 0 cgagtttcaa 0.994623 -85 ******* ** * Masking position 12 Map Score: 7.73662 Number of sites scoring better than the average of aligned sites = 35 Number in coding regions = 30 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 3 gacacaacttcagtcgattaaaattatttct 1 40 0 cagcgattaa 0.981584 -261 cgagtttttccagatgaccaagttcgacaag 1 110 0 cagtgaccaa 0.988235 -191 tttgaacccacaggagatcgaatcaggcgag 1 137 0 cagagatcga 0.995339 -164 caaagaactacagtagatcaagttgtagtaa 1 170 0 cagagatcaa 0.997895 -131 gaaaaaagttgagaagatcaaagaactacag 1 188 0 gagagatcaa 0.992625 -113 *** ******* Masking position 7 Map Score: 7.3893 Number of sites scoring better than the average of aligned sites = 7 Number in coding regions = 6 Number in noncoding regions = 1 Number of orfs with sites within 600 bp upstream = 1 Fraction of orfs with sites within 600 bp upstream = 0.000160617 Motif number 4 attttaatcgactgaagttgtgtcagcgag 1 47 1 actgaagttg 0.979537 -254 aacttgatctactgtagttctttgatcttc 1 177 1 actgtagttc 0.991799 -124 catggtcttaactttaatttgcactttagt 1 243 1 actttaattt 0.958663 -58 tttaatttgcactttagtttctcaaccttg 1 255 1 actttagttt 0.989313 -46 ********** Masking position 6 Map Score: 2.74331 Number of sites scoring better than the average of aligned sites = 26 Number in coding regions = 24 Number in noncoding regions = 2 Number of orfs with sites within 600 bp upstream = 1 Fraction of orfs with sites within 600 bp upstream = 0.000160617 Motif number 5 aattatttctaaacgcgtcaagagtctcta 1 20 0 aaacgcgtca 0.919785 -281 aactgtacataatctcgctgacacaacttc 1 60 0 aatctcgctg 0.972603 -241 ttttttattccaacttgtcgaacttggtca 1 97 1 caacttgtcg 0.933764 -204 tcatctggaaaaactcgcctgattcgatct 1 124 1 aaactcgcct 0.984377 -177 ccttttgtggaaactcggtgcatggtctta 1 223 1 aaactcggtg 0.972574 -78 ********** Masking position 2 Map Score: 1.2128 Number of sites scoring better than the average of aligned sites = 49 Number in coding regions = 42 Number in noncoding regions = 7 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 6 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 7 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 8 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0