AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i207_holliday_junction_mpneu_reg_300.orf -o207_mpneu_300.ace -a/home/amcguire/alignace/lib/ORF_mpneu.txt -z/skink1/amcguire/genomes/mpneu.fna -g0.40 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.4 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RMP00309 300 Mycoplasma_pneumoniae Motif number 1 taactgtcaataatctcttttttactcctg 1 8 0 taatcctttt 0.981262 -293 tattgacagttactcacgattttgaagtatata 1 29 1 tactccattt 0.981053 -272 cttctctatttcatctcttattatggatgtcga 1 80 0 tcatccttta 0.959985 -221 ttaatgggggtactcccttctctatttcatctc 1 96 0 tactccttct 0.987867 -205 ttaaccacattcattactattctggaattagta 1 125 1 tcattcatct 0.913924 -176 ctaacttcgatcatgttttttctgttactaatt 1 150 0 tcatgtttct 0.852382 -151 acttacacattcctcttaaatttcgcggtttga 1 252 0 tcctctattt 0.968049 -49 agtaaatctatcctcgcttatttacacat 1 282 1 tcctcctttt 0.995316 -19 ***** * * *** Masking position 4 Map Score: 7.42103 Number of sites scoring better than the average of aligned sites = 101 Number in coding regions = 79 Number in noncoding regions = 22 Number of orfs with sites within 600 bp upstream = 19 Fraction of orfs with sites within 600 bp upstream = 0.00305172 Motif number 2 caggagtaaaaaagagattattg 1 4 1 gagtaaaaaa 0.962219 -297 cacgattttgaagtatataacaccgacgac 1 43 1 aagtatataa 0.866068 -258 tactattctggaattagtaacagaaaaaac 1 139 1 gaattagtaa 0.888586 -162 aacatgatcgaagttagtagtaataattgc 1 166 1 aagttagtag 0.805709 -135 ggacacaaaaaacttaataaatggacaagt 1 196 1 aacttaataa 0.80532 -105 aactgatgcaaagaaaaaaacttgtccatt 1 215 0 aagaaaaaaa 0.80378 -86 ttcgcggtttgaaaaaataaactgatgcaa 1 234 0 gaaaaaataa 0.853483 -67 taagaggaatgtgtaagtaaatctatcctc 1 267 1 gtgtaagtaa 0.954559 -34 atgtgtaaataagcgaggatag 1 289 0 gtgtaaataa 0.962192 -12 ********** Masking position 9 Map Score: 4.94301 Number of sites scoring better than the average of aligned sites = 343 Number in coding regions = 280 Number in noncoding regions = 63 Number of orfs with sites within 600 bp upstream = 50 Fraction of orfs with sites within 600 bp upstream = 0.00803084 Motif number 3 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 4 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 5 ********** No masking Map Score: 7.91135e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0