AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i094_mgen_mpneu_100.orf -o094_mgen_mpneu_100.ace -a/home/amcguire/genomes/ORF_mgen.txt -z/home/amcguire/genomes/mgen.fna -g0.36 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.36 maxlen = 30 weight = 0.8 exclude = 1 Purged sequences: RMP00575 16 Mycoplasma_pneumoniae Input sequences: #1 RMG00209 112 Mycoplasma #2 RMG02317 20 Mycoplasma #3 RMG02322 24 Mycoplasma #4 RMG02325 35 Mycoplasma #5 RMG02326 90 Mycoplasma #6 RMP00579 300 Mycoplasma_pneumoniae Motif number 1 CAAAACAATGCAAAGTATTTTGGAATAACCAG 1 37 1 CAAAGTTTTG 0.715365 -76 TCTTTTGAGGAAAAGCAGTTATTATCTGGTTA 1 62 0 AAAAGCTTAT 0.731175 -51 TTTTTCACTGTAACTTCTTTTGAGGAAAAGCA 1 77 0 TAACTTTTTG 0.741184 -36 CAACTTAAAATCATTTTTTCACTGTAAC 1 95 0 AAAATCTTTT 0.598917 -18 AATTAATGGAAAAAGTTGCCTTCAAA 4 20 1 AAAAGTCCTT 0.932043 -16 GTGGCAATTTAAAAGTTACTTTAAACACCACC 5 25 1 AAAAGTCTTT 0.941495 -66 GAGCTATGTAAAAATCGGTCTTTTCAAAACAC 5 59 0 AAAATCTCTT 0.836539 -32 tgtaactcaaaaacgtaatttggattgcggta 6 46 0 aaacgttttg 0.747334 -255 ccgaatactgaaactttgttttgatggtcgac 6 174 1 aaactttttt 0.959396 -127 acgttctaaaaaacgtgtttttcgaccgcgtt 6 220 0 aaacgttttt 0.94708 -81 caacttgtgtaaaattcactttaaagaatcca 6 256 1 aaaattcttt 0.927789 -45 caatttattccaaattctcttgttc 6 286 1 caaattcttg 0.886005 -15 ****** **** Masking position 3 Map Score: 10.3423 Number of sites scoring better than the average of aligned sites = 462 Number in coding regions = 418 Number in noncoding regions = 44 Number of orfs with sites within 600 bp upstream = 34 Fraction of orfs with sites within 600 bp upstream = 0.00546097 Motif number 2 CAAAGTATTTTGGAATAACCAGATAATAAC 1 47 1 TGGAATAACC 0.984116 -66 TATTAGGCATTGGAATAACT 3 15 1 TGGAATAACT 0.982875 -10 GTTTAATTAATGGAAAAAGTTGCCTTCAAA 4 16 1 TGGAAAAAGT 0.875852 -20 cacgacatagcgcaataacacaagcacttt 6 17 1 cgcaataaca 0.843265 -284 tcgtcaaccatcgaacaaaaagacacacta 6 132 1 tcgaacaaaa 0.821221 -169 tttcagtattcggaacaaccgttgttagtg 6 157 0 cggaacaacc 0.966771 -144 cttaacgcggtcgaaaaacacgttttttag 6 217 1 tcgaaaaaca 0.915867 -84 caagagaatttggaataaattggattcttt 6 278 0 tggaataaat 0.939979 -23 ********** Masking position 5 Map Score: 6.01372 Number of sites scoring better than the average of aligned sites = 59 Number in coding regions = 53 Number in noncoding regions = 6 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 3 GTGGTGTTTAATTAATGGAA 4 1 1 GTGGTGTTTA 0.9921 -35 AAAACACCAGGTGGTGTTTAAAGTAACTTT 5 36 0 GTGGTGTTTA 0.9921 -55 TAAACACCACCTGGTGTTTTGAAAAGACCG 5 46 1 CTGGTGTTTT 0.9921 -45 cggtaaagtgcttgtgttattgcgctatgt 6 21 0 cttgtgttat 0.925981 -280 ********** Masking position 5 Map Score: 3.43124 Number of sites scoring better than the average of aligned sites = 21 Number in coding regions = 19 Number in noncoding regions = 2 Number of orfs with sites within 600 bp upstream = 1 Fraction of orfs with sites within 600 bp upstream = 0.000160617 Motif number 4 CTTTGGGATAGTGCTTCAGTCAT 1 3 1 TTGGGATGTG 0.986438 -110 TCCAAAATACTTTGCATTGTTTTGCTAATGA 1 30 0 TTTGCATGTT 0.943859 -83 AATTGCCACATTGTCATAGTCTT 5 3 0 TTGTCATGTC 0.949269 -88 aaaaacgtaatttggattgcggtaaagtgct 6 39 0 tttggatgcg 0.96977 -262 tacgtaaccttttggttcgtcaaccatcgaa 6 116 1 tttggttgtc 0.969257 -185 tgaaactttgttttgatggtcgaccgagatc 6 182 1 ttttgatgtc 0.977367 -119 ******* *** Masking position 7 Map Score: 2.92759 Number of sites scoring better than the average of aligned sites = 36 Number in coding regions = 35 Number in noncoding regions = 1 Number of orfs with sites within 600 bp upstream = 1 Fraction of orfs with sites within 600 bp upstream = 0.000160617 Motif number 5 ********** No masking Map Score: 7.69818e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: 7.69818e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 7 ********** No masking Map Score: 7.69818e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0