AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i263_mgen_mpneu_300.orf -o263_mgen_mpneu_300.ace -a/home/amcguire/genomes/ORF_mgen.txt -z/home/amcguire/genomes/mgen.fna -g0.36 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.36 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RMG04679 52 Mycoplasma #2 RMP00501 243 Mycoplasma_pneumoniae Motif number 1 ggacctggacagcacaaatggctgt 2 4 1 ctggcagcac 0.981454 -240 tagaattaaacaatttaagctctttgagaaaa 2 57 1 cattaagctc 0.984365 -187 tggaggggatcaaatgcaccaccaaagatcac 2 95 0 caatcaccac 0.980051 -149 gctttaatggcttgtacagcacgcttagcaat 2 143 0 ctgtcagcac 0.994219 -101 gctgtacaagccattaaagcacaaaagcttta 2 155 1 cattaagcac 0.996713 -89 tttaaatcaccgattaaagcatctaatcaagc 2 212 1 cattaagcat 0.975209 -32 attaaagcatctaatcaagcacgtttagca 2 224 1 caataagcac 0.995297 -20 * *** ****** Masking position 8 Map Score: 11.5298 Number of sites scoring better than the average of aligned sites = 29 Number in coding regions = 27 Number in noncoding regions = 2 Number of orfs with sites within 600 bp upstream = 1 Fraction of orfs with sites within 600 bp upstream = 0.000160617 Motif number 2 CTTGTGTCACAATTATTTTAGTTAGAATTA 1 15 1 AATTATTTTA 0.929376 -38 AGTTAGAATTAAATGTTTTAAGATCTTTT 1 34 1 AAATGTTTTA 0.982619 -19 ggacagcacaaatggctgtagctatcagga 2 17 1 aatggctgta 0.942234 -227 tagctatcaggattattttagttagaatta 2 35 1 gattatttta 0.777555 -209 aaagagcttaaattgtttaattctaactaa 2 52 0 aattgtttaa 0.911595 -192 attaaagcacaaaagctttactttgtacca 2 167 1 aaaagcttta 0.925685 -77 gtgatttaaaaaaggctttagcagttggta 2 192 0 aaaggcttta 0.982674 -52 gtgcttgattagatgctttaatcggtgatt 2 216 0 agatgcttta 0.960328 -28 ********** Masking position 10 Map Score: 8.44347 Number of sites scoring better than the average of aligned sites = 474 Number in coding regions = 428 Number in noncoding regions = 46 Number of orfs with sites within 600 bp upstream = 30 Fraction of orfs with sites within 600 bp upstream = 0.0048185 Motif number 3 TGCTCTTGTGTCACAATTATTTT 1 2 1 GCCTTGTTCA 0.972632 -51 gatagctacagccatttgtgctgtccaggtcc 2 11 0 gcatttggct 0.911554 -233 aacaatttaagctctttgagaaaaaaaatagt 2 65 1 gcctttggaa 0.990236 -179 atgtagatatgcgcttggtggaggggatcaaa 2 113 0 gccttgggga 0.988172 -131 ggcttgtacagcacgcttagcaatgtagatat 2 135 0 gccgcttgca 0.972415 -109 aaaggctttagcagttggtacaaagtaaagct 2 180 0 gcgttggaca 0.957968 -64 aactgctaaagccttttttaaatcaccgatta 2 196 1 gctttttaaa 0.7296 -48 atctaatcaagcacgtttagca 2 232 1 gccgtttgca 0.992522 -12 ** ***** *** Masking position 2 Map Score: 4.91157 Number of sites scoring better than the average of aligned sites = 92 Number in coding regions = 86 Number in noncoding regions = 6 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 4 AATTCTAACTAAAATAATTGTGACACAAGAGCA 1 11 0 AAAAAATTGA 0.995206 -42 aattctaactaaaataatcctgatagctacagc 2 31 0 aaaaaattga 0.995206 -213 ctctttgagaaaaaaaatagtgatctttggtgg 2 76 1 aaaaaattga 0.995206 -168 **** *** *** Masking position 7 Map Score: 1.90245 Number of sites scoring better than the average of aligned sites = 31 Number in coding regions = 26 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 5 Fraction of orfs with sites within 600 bp upstream = 0.000803084 Motif number 5 cttgtacagcacgcttagcaatgtagatat 2 135 0 acgcttagca 0.996965 -109 ctaatcaagcacgtttagca 2 234 1 acgtttagca 0.995505 -10 ********** Masking position 5 Map Score: 0.609702 Number of sites scoring better than the average of aligned sites = 3 Number in coding regions = 3 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: 7.34864e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 7 ********** No masking Map Score: 7.34864e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 8 ********** No masking Map Score: 7.34864e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0