AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i266_mgen_mpneu_100.orf -o266_mgen_mpneu_100.ace -a/home/amcguire/genomes/ORF_mgen.txt -z/home/amcguire/genomes/mgen.fna -g0.36 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.36 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RMP00504 194 Mycoplasma_pneumoniae Motif number 1 actagaatccgtggaacttcaggacttaac 1 18 0 gtggaacttc 0.994894 -177 tactggtaaaatgggaatttttcccattaa 1 51 0 atgggaattt 0.829404 -144 aagatcgattgtgaatattctttacttaat 1 89 0 gtgaatattc 0.936153 -106 aatattcacaatcgatcttctcctgctgcc 1 100 1 atcgatcttc 0.946375 -95 aataaaacgaatggaacgtgtcgtttgtga 1 153 1 atggaacgtg 0.963521 -42 cgtgtcgtttgtgaggcgtcgttttggacg 1 169 1 gtgaggcgtc 0.978712 -26 gtgggtcgtccaaaacgacg 1 185 0 gtgggtcgtc 0.996075 -10 ********** Masking position 2 Map Score: 6.81685 Number of sites scoring better than the average of aligned sites = 81 Number in coding regions = 70 Number in noncoding regions = 11 Number of orfs with sites within 600 bp upstream = 5 Fraction of orfs with sites within 600 bp upstream = 0.000803084 Motif number 2 atcttctcctgctgccgttttctcacgaca 1 114 1 gctgccgttt 0.992586 -81 attcattcaggatgtcgtgagaaaacggca 1 126 0 gatgtcgtga 0.937183 -69 acgacacgttccattcgttttattcattca 1 147 0 ccattcgttt 0.939298 -48 acgaatggaacgtgtcgtttgtgaggcgtc 1 159 1 cgtgtcgttt 0.995123 -36 tcgtttgtgaggcgtcgttttggacgaccc 1 173 1 ggcgtcgttt 0.994297 -22 ********** Masking position 8 Map Score: 3.52293 Number of sites scoring better than the average of aligned sites = 6 Number in coding regions = 6 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 3 aaaaatggttaagtcctgaagtt 1 4 1 aatggttaag 0.983887 -191 ctagtctattaatgggaaaaattcccattt 1 43 1 aatgggaaaa 0.988701 -152 tcaaattactactggtaaaatgggaatttt 1 60 0 actggtaaaa 0.983887 -135 cgacatcctgaatgaataaaacgaatggaa 1 139 1 aatgaataaa 0.936945 -56 ********** Masking position 3 Map Score: 1.86314 Number of sites scoring better than the average of aligned sites = 92 Number in coding regions = 84 Number in noncoding regions = 8 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 4 ********** No masking Map Score: 3.15071e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 5 ********** No masking Map Score: 3.15071e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: 3.15071e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0