1x Alkylation Buffer

Final Formulation:   

300mM Tris (pH 8.0)

50mM KCL   

(1) Mix the following:

167.4 mL of       1M Tris Acid

132.6 mL of        1M Tris Base

   25   mL of        2M KCl

(2) Fill to 1 liter with dH20

(3) Mix by shaking or with stir-bar.