Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.12239
Description:CGI-10 protein
Best-Of-UniGene (BOU) SequenceNM_015940
Genomic Coordiantes Displayed:Bases 626000 to 654000 of contig Hs14_10297_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.12239.jpeg
Best sense EST/protein match:BE315197 matched ref|XP_007436.1| (XM_007436) similar to Unknown (protein for MGC:23201) (H. sapiens) (E = e-127)
Best antisense EST/protein match:AA004882 matched ref|XP_058668.1| (XM_058668) hypothetical protein XP_058668 [Homo sapiens] (E = 5e-67)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW028387 cDNA clone IMAGE:2541239 stomach 3' read 1.6 kbProtein similarity
  R38921 cDNA clone IMAGE:24825 brain 3' read 1.8 kbProtein similarity
  AA004882 cDNA clone IMAGE:428569 pool 3' read 0.7 kbProtein similarityPolyadenylation signal
  AA677920 cDNA clone IMAGE:431001 pool 3' read Protein similarityPolyadenylation signal
  W88950 cDNA clone IMAGE:417420 pool 3' read Protein similarityPolyadenylation signal
  AA677748 cDNA clone IMAGE:430588 pool 3' read Protein similarityPolyadenylation signal
  AV689110 cDNA clone GKCDKA05 hepatocellular carcinoma 5' read Protein similarity
  AA005088 cDNA clone IMAGE:428569 pool 5' read 0.7 kbProtein similarity
  AA359171 cDNA clone ATCC:163274 lung 5' read Protein similarity
  AI754441 cDNA clone HBMSC_cr25d04 bone 3' read
  AW338878 cDNA clone IMAGE:2877932 pancreas 3' read Polyadenylation signal

SENSE ESTs

  BI759166 cDNA clone IMAGE:5182965 pooled colon, kidney, stomach 5' read Protein similarity
  BG437553 cDNA clone IMAGE:4621678 large cell carcinoma 5' read Protein similarity
  BG743635 cDNA clone IMAGE:4779094 skin 5' read Protein similarity
  AU099694 cDNA clone KAT00481 Protein similarity
  BI464126 cDNA clone IMAGE:5273138 testis, cell line 5' read Protein similarity
  BE513071 cDNA clone IMAGE:3545022 adenocarcinoma cell line 5' read Protein similarity
  BG824123 cDNA clone IMAGE:4878422 adenocarcinoma cell line 5' read Protein similarity
  BI821826 cDNA clone IMAGE:5176803 pooled brain, lung, testis 5' read Protein similarity
  BG823032 cDNA clone IMAGE:4867716 adenocarcinoma cell line 5' read Protein similarity
  BI766254 cDNA clone IMAGE:5202316 pooled lung and spleen 5' read Protein similarity
  BI755666 cDNA clone IMAGE:5197565 brain 5' read Protein similarity
  BI915213 cDNA clone IMAGE:5248474 brain 5' read Protein similarity
  BI769694 cDNA clone IMAGE:5205354 pooled lung and spleen 5' read Protein similarity
  BG391872 cDNA clone IMAGE:4537318 embryonal carcinoma, cell line 5' read Protein similarity
  R35543 cDNA clone IMAGE:37613 brain 5' read 1.7 kbProtein similarity
  BG745200 cDNA clone IMAGE:4850047 spleen 5' read Protein similarity
  Z46081 cDNA clone c2df02 brain Protein similarity