Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.167473
Description:phosphoglucomutase 5
Best-Of-UniGene (BOU) SequenceNM_021965
Genomic Coordiantes Displayed:Bases 201000 to 229000 of contig Hs9_19655_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.167473.jpeg
Best sense EST/protein match:NM_021965 matched ref|NP_068800.1| (NM_021965) phosphoglucomutase 5 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  R45181 cDNA clone IMAGE:34845 brain 3' read 1.3 kbProtein similarityPolyadenylation signal
  H12280 cDNA clone IMAGE:48182 brain 3' read 1.5 kbProtein similarity
  F03250 cDNA clone c1ra04 brain 3' read Protein similarity
  F04861 cDNA clone c1hc01 brain 3' read Protein similarity
  AI685066 cDNA clone IMAGE:2323659 pancreas 3' read 1.6 kbProtein similarity
  R19762 cDNA clone IMAGE:34845 brain 5' read 1.3 kb
  Z46017 cDNA clone c1hc01 brain 5' read
  F06976 cDNA clone c1ra04 brain 5' read