Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.170453
Description:tropomodulin
Best-Of-UniGene (BOU) SequenceNM_003275
Genomic Coordiantes Displayed:Bases 3124000 to 3306000 of contig Hs9_8633_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.170453.jpeg
Best sense EST/protein match:BE393232 matched gb|AAH02660.1|AAH02660 (BC002660) tropomodulin [Homo sapiens] (E = e-105)
Best antisense EST/protein match:BE784717 matched dbj|BAB71188.1| (AK056453) unnamed protein product [Homo sapiens] (E = 1e-89)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI567185 cDNA clone IMAGE:2191244 uterus 3' read 1.0 kbPolyadenylation signal
  AI951046 cDNA clone IMAGE:2548282 four pooled high-grade tumors, including two primary tumors 3' read Polyadenylation signal
  AA558423 cDNA clone IMAGE:1015778 germ cell 3' read Polyadenylation signal
  R38987 cDNA clone IMAGE:25081 brain 3' read 1.8 kbPolyadenylation signal
  AI570310 cDNA clone IMAGE:2184323 stomach 3' read 0.7 kbPolyadenylation signal
  AW058343 cDNA clone IMAGE:2544112 stomach 3' read Polyadenylation signal
  BI789270 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  AL044512 cDNA clone DKFZp434J182 testis 3' read Polyadenylation signal
  AI860385 cDNA clone IMAGE:2423749 uterus 3' read Polyadenylation signal
  AI583393 cDNA clone IMAGE:2228053 pancreas 3' read Polyadenylation signal
  AA001931 cDNA clone IMAGE:428146 pool 3' read Polyadenylation signal
  AI828284 cDNA clone IMAGE:2421944 pancreas 3' read Polyadenylation signal
  AA700429 cDNA clone IMAGE:460604 pool 3' read Polyadenylation signal
  AI799180 cDNA clone IMAGE:2265758 uterus 3' read 2.3 kbPolyadenylation signal
  AW768402 cDNA clone IMAGE:3002418 lymph 3' read Polyadenylation signal
  F09707 cDNA clone c35c12 brain 3' read Polyadenylation signal
  AW591000 cDNA clone IMAGE:2703250 uterus 3' read Polyadenylation signal
  AI684242 cDNA clone IMAGE:2275887 uterus 3' read Polyadenylation signal
  BI494941 cDNA clone IMAGE:2539624 ear 3' read Polyadenylation signal
  AA001731 cDNA clone IMAGE:428146 pool 5' read
  BF969167 cDNA clone IMAGE:4357959 adrenal cortex carcinoma, cell line 5' read
  AA330142 cDNA clone ATCC:131845 whole embryo 5' read
  AL044511 cDNA clone DKFZp434J182 testis 5' read
  BI962789 cDNA clone (no-name) islets of langerhans 5' read
  N86735 cDNA clone L0048 heart 5' read
  BI962601 cDNA clone (no-name) islets of langerhans 5' read
  T66336 cDNA clone IMAGE:21877 brain 5' read 1.7 kb
  BG391984 cDNA clone IMAGE:4538671 embryonal carcinoma, cell line 5' read
  F12066 cDNA clone c35c12 brain
  AV692564 cDNA clone GKCCNB01 hepatocellular carcinoma 5' read
  AV686037 cDNA clone GKCALH10 hepatocellular carcinoma 5' read
  BE299365 cDNA clone IMAGE:3029584 rhabdomyosarcoma 5' read
  BF673393 cDNA clone IMAGE:4272519 prostate 5' read
  BG766505 cDNA clone IMAGE:4869112 melanotic melanoma, high mdr (cell line) 5' read
  AA337198 cDNA clone ATCC:138927 uterus 5' read
  R57574 cDNA clone F3597 heart 5' read
  BG818819 cDNA clone IMAGE:4914762 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BE784717 cDNA clone IMAGE:3876675 large cell carcinoma 5' read Protein similarity
  AW149071 cDNA clone IMAGE:2617582 kidney 3' read
  BG282137 cDNA clone IMAGE:4545571 melanotic melanoma 5' read
  BG107185 cDNA clone IMAGE:4385905 lymphoma, cell line 5' read

SENSE ESTs

  AI879136 cDNA clone IMAGE:2518652 frontal lobe 5' read Protein similarity
  BI544545 cDNA clone IMAGE:5284435 hippocampus, cell line 5' read Protein similarity
  BG700995 cDNA clone IMAGE:4814610 hippocampus, cell line 5' read Protein similarity
  BI918024 cDNA clone IMAGE:5245841 brain 5' read Protein similarity
  AU100413 cDNA clone adSU01679 Protein similarity
  BG753672 cDNA clone IMAGE:4876360 normal pigmented retinal epithelium 5' read Protein similarity
  BE379271 cDNA clone IMAGE:3610014 endometrium, adenocarcinoma cell line 5' read Protein similarity
  BI768308 cDNA clone IMAGE:5203321 pooled lung and spleen 5' read Protein similarity
  AU076873 cDNA clone kaia2086
  BI522500 cDNA clone IMAGE:5239649 brain 5' read Protein similarity
  BI915726 cDNA clone IMAGE:5248373 brain 5' read Protein similarity
  BE393232 cDNA clone IMAGE:3626601 endometrium, adenocarcinoma cell line 5' read Protein similarity
  AL134249 cDNA clone DKFZp547E044 brain 5' read Protein similarity
  BG926672 cDNA clone (no-name) cartilage Protein similarity
  N85104 cDNA clone J2320 heart 5' read Protein similarity
  BG703244 cDNA clone IMAGE:4817860 hippocampus, cell line 5' read Protein similarity
  BF347015 cDNA clone IMAGE:4157185 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BF526685 cDNA clone IMAGE:4213348 glioblastoma with egfr amplification 5' read Protein similarity
  BG700839 cDNA clone IMAGE:4814122 hippocampus, cell line 5' read Protein similarity
  BE612669 cDNA clone IMAGE:3856370 adenocarcinoma 5' read Protein similarity
  AA682365 cDNA clone IMAGE:462923 pool 3' read Polyadenylation signal
  H16448 cDNA clone IMAGE:47848 brain 5' read 1.9 kb
  AI248570 cDNA clone IMAGE:1849651 pool 3' read 0.7 kb
  AA776437 cDNA clone IMAGE:453751 pool 3' read Polyadenylation signal
  R58304 cDNA clone G1681 heart 5' read
  AA703565 cDNA clone IMAGE:450256 pool 3' read
  AI537366 cDNA clone IMAGE:2187130 stomach 3' read 1.6 kbPolyadenylation signal
  AI052647 cDNA clone IMAGE:1675661 pool 3' read 0.7 kb
  AA676336 cDNA clone IMAGE:430517 pool 3' read Polyadenylation signal
  AW102830 cDNA clone IMAGE:2596096 ovary 3' read Polyadenylation signal
  R51646 cDNA clone IMAGE:39671 brain 3' read 1.4 kbPolyadenylation signal
  AW591890 cDNA clone IMAGE:2839058 uterus 3' read Polyadenylation signal
  AW583061 cDNA clone (no-name) islets of langerhans 5' read
  BF340371 cDNA clone IMAGE:4184874 glioblastoma with egfr amplification 5' read
  BG776282 cDNA clone IMAGE:4808412 mucoepidermoid carcinoma 5' read
  BF525704 cDNA clone IMAGE:4212825 glioblastoma with egfr amplification 5' read
  R25568 cDNA clone IMAGE:36435 brain 5' read 1.1 kb
  AI432035 cDNA clone IMAGE:2120176 pancreas 3' read Polyadenylation signal
  BE613276 cDNA clone IMAGE:3856370 adenocarcinoma 3' read
  AA902904 cDNA clone IMAGE:1516686 smooth muscle 3' read 0.8 kbPolyadenylation signal
  BE394733 cDNA clone IMAGE:3658785 endometrium, adenocarcinoma cell line 5' read
  H16249 cDNA clone IMAGE:47848 brain 3' read 1.9 kbPolyadenylation signal
  AI446392 cDNA clone IMAGE:2141134 stomach 3' read 0.7 kbPolyadenylation signal
  AI689560 cDNA clone IMAGE:2276274 uterus 3' read 1.7 kbPolyadenylation signal
  F19371 cDNA clone NOTAVAIL03973
  F26389 cDNA clone s4000053F01 muscle
  C05065 cDNA clone 3NHC4566 heart
  F36802 cDNA clone sH5000020-0/D05 muscle
  F21166 cDNA clone 043X4-30 muscle
  R58343 cDNA clone G2467 heart 5' read
  BE046883 cDNA clone IMAGE:3124381 squamous cell carcinoma 3' read