Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.200188
Description:deleted in lung and esophageal cancer 1
Best-Of-UniGene (BOU) SequenceNM_007338
Genomic Coordiantes Displayed:Bases 798000 to 812000 of contig Hs3_6179_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.200188.jpeg
Best sense EST/protein match:NM_007338 matched ref|NP_005097.1| (NM_005106) deleted in lung and esophageal cancer 1, isoform (E = 0.0)
Best antisense EST/protein match:AI860341 matched ref|NP_001598.1| (NM_001607) acetyl-Coenzyme A acyltransferase 1; acetyl-Coenzyme A (E = 5e-27)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI860341 cDNA clone IMAGE:2423692 uterus 3' read 1.0 kbProtein similarity
  AI280490 cDNA clone IMAGE:1963674 ovary 3' read 1.8 kb
  AI824547 cDNA clone IMAGE:2275058 uterus 3' read 1.8 kb
  AW072589 cDNA clone IMAGE:2568951 five pooled sarcomas, including myxoid liposarcoma, solitary 3' read
  T62904 cDNA clone IMAGE:86074 liver 3' read 0.6 kb
  W87636 cDNA clone IMAGE:417115 pool 3' read 0.7 kb

SENSE ESTs

  BI560283 cDNA clone IMAGE:5295796 testis, cell line 5' read Protein similarity
  BG721487 cDNA clone IMAGE:4827160 testis, cell line 5' read
  AL039638 cDNA clone DKFZp434G1111 testis 5' read Protein similarity
  BF341391 cDNA clone IMAGE:4149253 glioblastoma with egfr amplification 5' read Protein similarity
  AI560858 cDNA clone IMAGE:2211335 uterus 3' read 7.0 kb