Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.23856
Description:hypothetical protein MGC5297
Best-Of-UniGene (BOU) SequenceNM_024091
Genomic Coordiantes Displayed:Bases 95089 to 108746 of contig Hs5_6733_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.23856.jpeg
Best sense EST/protein match:NM_024091 matched ref|NP_076996.1| (NM_024091) hypothetical protein MGC5297 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF337032 cDNA clone IMAGE:4182652 glioblastoma with egfr amplification 5' read
  F10134 cDNA clone c3ba03 brain 3' read
  Z38251 cDNA clone c04d10 brain 3' read Protein similarityPolyadenylation signal
  F04106 cDNA clone c2jg10 brain 3' read Polyadenylation signal
  R45293 cDNA clone IMAGE:35627 brain 3' read 1.2 kbProtein similarityPolyadenylation signal
  R37102 cDNA clone IMAGE:25728 brain 3' read 1.2 kbProtein similarityPolyadenylation signal
  R50752 cDNA clone IMAGE:38510 brain 3' read 1.3 kbProtein similarityPolyadenylation signal
  T87208 cDNA clone IMAGE:22482 brain 3' read 1.6 kbPolyadenylation signal

SENSE ESTs

  BG499460 cDNA clone IMAGE:4669244 adenocarcinoma 5' read
  BG529738 cDNA clone IMAGE:4698792 embryonal carcinoma 5' read Protein similarity
  AI805496 cDNA clone IMAGE:2269156 uterus 3' read 1.8 kbProtein similarityPolyadenylation signal
  BG289645 cDNA clone IMAGE:4513637 transitional cell papilloma, cell line 5' read
  BG530364 cDNA clone IMAGE:4697305 embryonal carcinoma 5' read Protein similarity
  AV736217 cDNA clone CBFASA06 cord blood 5' read
  BG615709 cDNA clone IMAGE:4773955 embryonal carcinoma 5' read Protein similarity
  BF571885 cDNA clone IMAGE:4244157 melanotic melanoma, high mdr 5' read
  BI754398 cDNA clone IMAGE:5197386 brain 5' read Protein similarity
  BF541811 cDNA clone IMAGE:4067349 hypernephroma 5' read
  BI963142 cDNA clone (no-name) islets of langerhans 3' read Protein similarityPolyadenylation signal
  BF028571 cDNA clone IMAGE:3995997 carcinoma, cell line 5' read
  BG613096 cDNA clone IMAGE:4771591 embryonal carcinoma 5' read
  BG613049 cDNA clone IMAGE:4771625 embryonal carcinoma 5' read Protein similarity
  BF665182 cDNA clone IMAGE:4275720 primitive neuroectoderm 5' read
  BG167496 cDNA clone IMAGE:4455651 hypernephroma, cell line 5' read
  BG533707 cDNA clone IMAGE:4700295 liver 5' read Protein similarity
  BG504509 cDNA clone IMAGE:4664757 embryonal carcinoma 5' read
  BI964729 cDNA clone (no-name) islets of langerhans 5' read
  AA314900 cDNA clone ATCC:112147 5' read
  BE537873 cDNA clone IMAGE:3453776 placenta 5' read
  BG388682 cDNA clone IMAGE:4521950 embryonal carcinoma, cell line 5' read
  AA316122 cDNA clone ATCC:111803 5' read