Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.250493
Description:zinc finger protein 219
Best-Of-UniGene (BOU) SequenceNM_016423
Genomic Coordiantes Displayed:Bases 5317000 to 5338000 of contig Hs14_19739_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.250493.jpeg
Best sense EST/protein match:NM_016423 matched ref|NP_057507.1| (NM_016423) zinc finger protein 219 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:AI805536 matched ref|XP_033519.2| (XM_033519) hypothetical protein XP_033519 [Homo sapiens] (E = 2e-61)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI805351 cDNA clone IMAGE:2276364 uterus 3' read 1.9 kbProtein similarity
  AI561252 cDNA clone IMAGE:2210068 uterus 3' read 1.9 kbProtein similarity
  AI805536 cDNA clone IMAGE:2276460 uterus 3' read 1.2 kbProtein similarity
  AW511447 cDNA clone IMAGE:2805999 uterus 3' read Polyadenylation signal
  AW471304 cDNA clone IMAGE:2832118 pancreas 3' read Polyadenylation signal
  AA705153 cDNA clone IMAGE:461613 pool 3' read Polyadenylation signal
  AA699723 cDNA clone IMAGE:433308 pool 3' read Polyadenylation signal
  T72160 cDNA clone IMAGE:85888 liver 3' read Polyadenylation signal